Identification of a Homeobox Gene and a Keratin Pair Expressed in Amphibian Limb Regeneration

  • J. B. Brockes
  • P. Ferretti
  • P. Savard
Part of the NATO ASI Series book series (NSSA, volume 172)


We have identified a newt homeobox gene called NvHboxl which is the homologue of Xenopus Hboxl and human HHO.C8. The gene is expressed in the limb and limb blastema of the newt Notophthalmus viridescens, as a transcript of 1.8 kb. It is of interest that the transcript is expressed in a related but distinct form in tail tissues, that it is expressed in adult limb of the newt but not of Xenopus, and that its expression is higher in a proximal than a distal blastema.

By using specific monoclonal antibodies, we have detected transient expression of the keratin pair 8 and 18 in mesenchymal cells of the newt blastema. While the majority of blastemal cells express these antigens, they are not detectable in sections of the developing limb bud of newt embryos. Keratin 8 and 18, which are usually expressed in simple epithelia, represent a new marker for regeneration versus development.


Homeobox Gene Blastemal Cell Preimplantation Mouse Embryo Simple Epithelium Tail Tissue 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • J. B. Brockes
    • 1
  • P. Ferretti
    • 1
  • P. Savard
    • 1
  1. 1.Ludwig Institute for Cancer ResearchLondonEngland

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