In Vitro Effects of a Mitogenic Factor on Xenopus Laevis Forelimb Regeneration
The essential role of nerves in the support of amphibian forelimb regeneration is well documented (Singer, 1952; 1954). In vitro experiments (Globus and Vethamany-Globus, 1977; Tomlinson et al., 1981) suggest that the neurotrophic agent is a diffusible proteinaceous nerve growth factor of low molecular weight, which is inactivated by heat and trypsin (Singer, 1976). The current investigation was designed to test the effects of a chick brain growth factor (CBGF) on Xenopus laevis forelimb regeneration; specifically its role as a mitogenic factor for mesenchyme-like cells in the regenerate. CBGF is a low molecular weight (500–2, 000), relatively heat stable peptide (Carlone et al., 1988).
KeywordsMitogenic Factor Blastema Cell Regeneration Blastema Glial Growth Factor Neurotrophic Agent
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