In Vitro Effects of a Mitogenic Factor on Xenopus Laevis Forelimb Regeneration

  • Catherine Tsilfidis
  • Richard A. Liversage
Part of the NATO ASI Series book series (NSSA, volume 172)


The essential role of nerves in the support of amphibian forelimb regeneration is well documented (Singer, 1952; 1954). In vitro experiments (Globus and Vethamany-Globus, 1977; Tomlinson et al., 1981) suggest that the neurotrophic agent is a diffusible proteinaceous nerve growth factor of low molecular weight, which is inactivated by heat and trypsin (Singer, 1976). The current investigation was designed to test the effects of a chick brain growth factor (CBGF) on Xenopus laevis forelimb regeneration; specifically its role as a mitogenic factor for mesenchyme-like cells in the regenerate. CBGF is a low molecular weight (500–2, 000), relatively heat stable peptide (Carlone et al., 1988).


Mitogenic Factor Blastema Cell Regeneration Blastema Glial Growth Factor Neurotrophic Agent 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Catherine Tsilfidis
    • 1
  • Richard A. Liversage
    • 1
  1. 1.Ramsay Wright Zoological LaboratoriesUniversity of TorontoCanada

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