Insulin Localization in the Pancreas and in Forelimb Regenerates of the Adult Newt, Notophthalmus Viridescens
Metabolic hormones play an essential role in the regeneration of urodele appendages. Indeed, the involvement of insulin is essential as organ-cultured adult newt forelimb blastemata attain maximum growth and differentiation only when insulin is present in combination with thyroxine, hydrocortisone, and either growth hormone (1) or prolactin (2) . In experimentally induced diabetic adult newts, forelimb blastemata fail to accumulate the critical mass of cells reguired for the continuation of regeneration. Also, cartilage formation as well as growth and differentiation of the tissues are markedly reduced (3, 4). Given the importance of insulin to the process of regeneration, it was of interest to attempt localization of this hormone at its source (presumably the pancreas) and at its site of action in the regenerating forelimb.
KeywordsScanning Electron Micro Insulin Antibody Adult Newt Immunogold Localization Notophthalmus VIRIDESCENS
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