The Nerve Growth Factor Proteins of the Mouse Submaxillary Gland
The proteins which elicit nerve growth factor NGF activity have been the object of interest and investigation for more than a decade. The discovery of a factor which stimulates differentiation of certain neurons in embryonic sensory and sympathetic ganglia (Levi-Montalcini, 1966) and its characterization as a protein (Cohen & Levi-Montalcini, 1957) were events of very considerable significance in neurobiology and their consequences are still being exploited. Soon after the protein character of NGF was established it was found that very much more NGF activity could be obtained from the adult male mouse submaxillary gland than from the original tumor material (Cohen, 1960) and the gland became the preferred starting material for further investigation. The original method for isolating NGF from this gland was devised by Cohen (1960). His preparation was optimally active at about 15 ng/ml in the in vitro plasma clot assay and sedimented at 4.3S.
KeywordsNerve Growth Factor Submaxillary Gland Subunit Type Female Gland Mouse Submaxillary Gland
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