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The Nerve Growth Factor Proteins of the Mouse Submaxillary Gland

  • Silvio Varon
  • E. M. Shooter

Abstract

The proteins which elicit nerve growth factor NGF activity have been the object of interest and investigation for more than a decade. The discovery of a factor which stimulates differentiation of certain neurons in embryonic sensory and sympathetic ganglia (Levi-Montalcini, 1966) and its characterization as a protein (Cohen & Levi-Montalcini, 1957) were events of very considerable significance in neurobiology and their consequences are still being exploited. Soon after the protein character of NGF was established it was found that very much more NGF activity could be obtained from the adult male mouse submaxillary gland than from the original tumor material (Cohen, 1960) and the gland became the preferred starting material for further investigation. The original method for isolating NGF from this gland was devised by Cohen (1960). His preparation was optimally active at about 15 ng/ml in the in vitro plasma clot assay and sedimented at 4.3S.

Keywords

Nerve Growth Factor Submaxillary Gland Subunit Type Female Gland Mouse Submaxillary Gland 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. ATTARDI, D. A., SCHLESINGER, M. J., & SCHLESINGER, S. Submaxillary gland of mouse: Properties of a purified protein affecting muscle tissue in vitro. Science, 1967, 156, 1253–1255.PubMedCrossRefGoogle Scholar
  2. BOCCHINI, V., & ANGELETTI, P. U. The nerve growth factor: Purification as a 30,000-molecular-weight protein. Proceedings of the National Academy of Sciences, 1969, 64, 787–794.CrossRefGoogle Scholar
  3. BOEKER, E. A., & SNELL, E. E. Arginine decarboxylase from Escherichia coli. II. Dissociation and reassociation of subunits. Journal of Biological Chemistry, 1968, 243, 1678–1684.PubMedGoogle Scholar
  4. BURGESS, R. R., TRAVERS, A. A., DUNN, J. J., & BAUTZ, E. K. F. Factor stimulating transcription by RNA polymerase. Nature, 1969, 221, 43–36.PubMedCrossRefGoogle Scholar
  5. CALISSANO, P., & ANGELETTI, P. U. Testosterone effect on the synthetic rate of two estero-peptidases in the mouse submaxillary gland. Biochimica et Biophysica Acta, 1968, 156, 51–58.PubMedCrossRefGoogle Scholar
  6. COHEN, S. Purification of a nerve growth promoting protein from the mouse salivary gland and its neurocyte toxic antiserum. Proceedings of the National Academy of Sciences, 1960, 46, 302–310.CrossRefGoogle Scholar
  7. COHEN, S., & LEVI-MONTALCINI, R. Purification and properties of a nerve growth-promoting factor isolated from mouse sarcoma 180. Cancer Research, 1957, 17, 15–20.PubMedGoogle Scholar
  8. CREIGHTON, T. E., & YANOFSKY, C. Association of the α and β2 tryptophan synthetase of Escherichia coli. Journal of Biological Chemistry, 1966, 241, 980–990.PubMedGoogle Scholar
  9. GREENE, L. A., SHOOTER, E. M., & VARON, S. Enzymatic activities of mouse nerve growth factor and its subunits. Proceedings of the National Academy of Sciences, 1968, 60, 1383–1388.CrossRefGoogle Scholar
  10. GREENE, L. A., SHOOTER, E. M., & VARON, S. Subunit interaction and enzymatic activity of mouse 7S nerve growth factor. Biochemistry, 1969, 8, 3735–3741.PubMedCrossRefGoogle Scholar
  11. GROSSMAN, A., LELE, K. P., SHELDON, I., SCHENKEIN, I., & LEVY, M. The effect of esteroproteases from submaxillary gland on the growth of rat hepatoma cells in tissue culture. Experimental Cell Research, 1969, 54, 260–263.PubMedCrossRefGoogle Scholar
  12. GUIDOTTI, G. Studies on the chemistry of hemoglobin. III. The interactions of the αβ subunits of hemoglobin. Journal of Biological Chemistry, 1967, 242, 3694–3703.PubMedGoogle Scholar
  13. GUIDOTTI, G., KONIGSBERG, W., & CRAIG, L. C. On the dissociation of normal adult human hemoglobin. Proceedings of the National Academy of Sciences, 1963, 50, 774–782.CrossRefGoogle Scholar
  14. HOFFMAN, H. Biological significance and action of the “nerve growth” factor. In R. E. Bowman and S. P. Datta (Eds.), Biochemistry of Brain and Behavior. New York: Plenum Press, 1970.Google Scholar
  15. HOFFMAN, H., & MCDOUGALL, J. Some biological properties of proteins of the mouse submaxillary gland as revealed by growth of tissues on electrophoretic acrylamide gels, Experimental Cell Research, 1968, 51, 485–503.PubMedCrossRefGoogle Scholar
  16. HOFFMAN, H., NAUGHTON, M. A., MCDOUGALL, J., & HAMILTON, E. A. Nerve growth factor and a thymus inhibitor, separations by tissue culture on acrylamide gels. Nature, 1967, 214, 703–705.PubMedCrossRefGoogle Scholar
  17. LEVI-MONTALCINI, R. The nerve growth factor, its mode of action on sensory and sympathetic nerve cells. Harvey Lecture Series, 1966, 60, 217–259.Google Scholar
  18. LEVI-MONTALCINI, R., MEYER, H., & HAMBURGER, V. In vitro experiments on the effect of mouse sarcoma 180 and 37 on the spinal and sympathetic ganglia of the chick embryo. Cancer Research, 1954, 14, 49–57.PubMedGoogle Scholar
  19. SHAPIRO, A. L., VINUELA, E., & MAIZEL, J. V. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communication, 1967, 28, 815–820.CrossRefGoogle Scholar
  20. SHOOTER, E. M., & VARON, S. Macromolecular aspects of the nerve growth factor proteins. In A. Lajtha (Ed.), Protein Metabolism of the Nervous System. New York: Plenum Press, 1970.Google Scholar
  21. SHOOTER, E. M., VARON, S., & NOMURA, J. The nerve growth factor protein and its subunits. Chimia, 1968, 22, 144.Google Scholar
  22. SMITH, A. P., VARON, S., & SHOOTER, E. M. Multiple forms of the nerve growth factor protein and its subunits. Biochemistry, 1968, 7, 3259–3268.PubMedCrossRefGoogle Scholar
  23. SMITH, A. P., GREENE, L. A., FISK, H. R., VARON, S., & SHOOTER, E. M. Dissociation equilibria in 7S NGF and its subunits. Biochemistry, 1969, 8, 4918–4926.PubMedCrossRefGoogle Scholar
  24. TAYLOR, J., COHEN, S., & MITCHELL, W. Epidermal growth factor (EGF): Properties of a high molecular weight species. Federation Proceedings, 1970, 29, 670.Google Scholar
  25. VARON, S. The investigation of neural development by experimental in vitro techniques. In D. Pease (Ed.), Cellular Aspects of Neural Growth and Differentiation. UCLA Forum Med. Sci. No. 14, Los Angeles, California: University of California Press, 1970, in press.Google Scholar
  26. VARON, S., NOMURA, J., & SHOOTER, E. M. The isolation of the mouse nerve growth factor protein in a high molecular weight form. Biochemistry, 1967, 6, 2202–2209.PubMedCrossRefGoogle Scholar
  27. VARON, S., NOMURA, J., & SHOOTER, E. M. Reversible dissociation of the mouse nerve growth factor protein into different subunits. Biochemistry, 1968, 7, 1296–1303.PubMedCrossRefGoogle Scholar
  28. ZANINI, A., ANGELETTI, P., & LEVI-MONTALCINI, R. Immunochemical properties of the nerve growth factor. Proceedings of the National Academy of Sciences, 1968, 61, 835–842.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Silvio Varon
    • 1
  • E. M. Shooter
    • 2
  1. 1.Department of BiologyUniversity of California at San DiegoLa JollaUSA
  2. 2.Departments of Genetics and BiochemistryStanford UniversityStanfordUSA

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