The Influence of Age, Proteoglycans and UV-Irradiation on Biochemical and Rheological Parameters of Rat’s Skin

  • Hans Niedermüller
  • Rolf-D. Petersen
Part of the Advances in Experimental Medicine and Biology book series (NATO ASI F, volume 231)


Skin aging is accompanied by biochemical, morphological and Theological changes (Banga, 1966, 1972; Fleischmajer et al., 1973; Hall, 1976; Viidik, 1973, 1982). Many people desire to reduce these senile changes as there are an increase of collagen concentration (Gillman, 1970) with a concomitant decrease of total collagen due to skin thinning (Viidik, 1982), a decrease of soluble collagen (Harris and Sjoerdsma, 1966; Verzár, 1964) by higher crosslinking of collagen, leading to an increasing fibre stiffness (Bailey and Robins, 1973). Moreover, there is a loss of ground substance (proteoglycans (PG), mainly hyaluronate and dermatan sulphate, as first stated by Clausen (1962)), and therefore we observe a decreasing hydration of the matrix by dysregulation of homeostasis. The content of elastin increases (Pearce and Grimmer, 1972), and there are pronounced changes in the network of elastic fibres (Viidik, 1982). All these changes are manifested phenomenologically in the formation of more wrinkles, increasing dryness and, mechanically, in a loss of elasticity. Changes of the matrix result from genetically scheduled enzymatic mechanisms (Hayflick, 1982), but more often from stochastic processes with an endogenous or an exogenous origin.


Elastic Fibre Rheological Parameter Hysteresis Curve Dermatan Sulphate Dorsal Skin 
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  1. Bailey, A.J., and Robins, S.P., 1973, Development and Maturation of the Crosslinks of the Collagen Fibres of the Skin, in: “Front.Matrix Biol., Vol. 1”, L. Robert, P. Robert, ed., Karger, Basel.Google Scholar
  2. Banga, I., 1966, “Structure and Function of Elastin and Collagen”, Akadémiai Kiadó, Budapest.Google Scholar
  3. Banga, I., 1972, Methoden zur Untersuchung der Veränderungen der physikalischen Eigenschaften des Bindegewebes im Altersgang, Z. Alternsforsch., 25:247.PubMedGoogle Scholar
  4. Blix, G., 1948, The determination of hexosamines, Acta chemscand., 2:476.Google Scholar
  5. Clausen, B., 1962, Influence of age on connective tissue, Lab.Invest., 11:229.PubMedGoogle Scholar
  6. Danielsen, L., 1979, “Morphological Changes in Pseudoxanthoma Elasticum and Senile Skin”, FADL Publication Co., Copenhagen.Google Scholar
  7. Fleischmajer, R., Perlish, J.S., and Bashey, R.I., 1973, Aging of Human Dermis, in: “Front.Matrix Biol., Vol.1”, L. Robert, P. Robert, ed., Karger, Basel.Google Scholar
  8. Gillman, T., 1970, “The Dermis”, Blackwell Scientific Publications, London.Google Scholar
  9. Hall, D.A., 1976, “The Aging of Connective Tissue”, Acad.Press, New York.Google Scholar
  10. Harris, E., and Sjoerdsma, A., 1966, Collagen content, solubility and cross-linking in human dermis, J.clin.Invest., 45:1020.Google Scholar
  11. Hayflick, L., 1982, Ageing and Death of Vertebrate Cells, in: “Lectures on Gerontology, Vol.I/A”, Acad.Press, New York.Google Scholar
  12. Heikkinen, E., 1968, Transformation of rat skin collagen, Acta physiol.scand., 74, suppl.317:1.CrossRefGoogle Scholar
  13. Heikkinen, E., 1973, Ageing of Interstitial Collagen, in: “Front.Matrix Biol., Vol.1”, L. Robert, P. Robert, ed., Karger, Basel.Google Scholar
  14. Jarret, A., 1974, “The Physiology and Pathophysiology of Skin, Vol.3”, Acad. Press, London and New York.Google Scholar
  15. Niedermüller, H., Skalicky, M., Hofecker, G., and Kment, A., 1977, Investigations on the kinetics of collagen-metabolism in young and old rats, Exp.Gerontol., 12:159.PubMedCrossRefGoogle Scholar
  16. Pav, E., Kment, A., Hofecker, G., Tielsch, W., and Niedermüller, H., 1975, Dehnungsmessungen an der Rattenaorta I, akt.gerontol., 5:453.Google Scholar
  17. Pav, E., Hofecker, G., Kment, A., and Niedermüller, H., 1977, Dehnungsmessungen an der Rattenaorta II, akt.gerontol., 7:529.Google Scholar
  18. Pav, E., Hofecker, G., Kment, A., Skalicky, M., and Niedermüller, H., Dehnungsmessungen an der Rattenaorta III, akt.gerontol., 8:261.Google Scholar
  19. Pearce, R.H., and Grimmer, B.J., 1972, Age and the chemical constitution of normal human dermis, J.invest.Derm., 58:347.PubMedCrossRefGoogle Scholar
  20. Remington, J.W., 1955, Hysteresis loop behaviour of the aorta and other extensible tissues, Amer.J.Physiol., 180:83.PubMedGoogle Scholar
  21. Robert, B., and Robert, L., 1973, Aging of Connective Tissues. General Considerations, in: “Front.Matrix Biol., Vol.1”, L. Robert, P. Robert, ed., Karger, Basel.Google Scholar
  22. Selmanowitz, V.J., Rizer, R.L., Orentreich, L., 1977, Aging of the Skin and its Appendages, in: “Handbook of the Biology of Aging”, C.E. Finch, L. Hayflick, ed., Van Nostrand Reinhold, New York.Google Scholar
  23. Shuster, S., and Bottoms, E., 1967, Effect of Ultra-violet Radiation on Skin Collagen of Intact Living Mice, Nature, 214:599.CrossRefGoogle Scholar
  24. Stegemann, H., 1958, Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd, Z.Physiol.Chem., 311:41.CrossRefGoogle Scholar
  25. Verzar, F., 1964, Ageing of the collagen fibre, Int.Rev.Conn.Tissue Res., 2:243.Google Scholar
  26. Viidikj, A., 1973, Rheology of Skin with Special Reference to Age-Related Parameters and their Possible Correlation to Structure, in: “Front.Matrix Biol., Vol.l”, L. Robert, P. Robert, ed., Karger, Basel.Google Scholar
  27. Viidik, A., 1980, Mechanical Properties of Parallel-fibred Collagenous Tissues, in: “Biology of Collagen”, A. Viidik, J. Vuust, ed., Acad.Press, London and New York.Google Scholar
  28. Viidik, A., 1982, Age-related Changes in Connective Tissues, in: “Lectures on Gerontology, Vol.1”, A. Viidik, ed., Acad.Press, London and New York.Google Scholar
  29. Vogel, H.G., 1976, Altersabhängige Veränderungen der mechanischen und biochemischen Eigenschaften der Cutis bei Ratten, akt.gerontol., 6:477.Google Scholar
  30. Vogel, H.G., 1978, Age Dependence of Mechanical Parameters in Rat Skin Following Repeated Strain, akt.gerontol., 8:601.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Hans Niedermüller
    • 1
  • Rolf-D. Petersen
    • 2
  1. 1.Dept.of GerontologyUniv.of Veterinary MedicineWienAustria
  2. 2.Chemisches Laboratorium Dr. Kurt Richter GmbHBerlin 41 (West)Germany

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