Post-Translational Formation of Hypusine: Biogenesis of Translation Initiation Factor eIF-4D
Hypusine is a derivative of lysine which occurs in a single cellular protein, eIF-4D1. This unusual basic amino acid was discovered in 1971 in extracts of bovine brain by Shiba and coworkers, who determined its chemical structure as Nε-[4-amino-2-hydroxybutyl]lysine.2 Subsequently, its occurrence as the free amino acid and as a component of protein in various animal tissues was reported. Ten years later, we observed radiolabeling of a single protein (Mr; ~18,000, pI; ~5.3) in human peripheral lymphocytes following their incubation with [3H]spermidine and identified the radioactive component of this protein as hypusine.5 Based on this finding we commenced systematic studies of the hypusine-containing protein and of the mechanism and regulation of the biosynthesis of hypusine.
KeywordsChinese Hamster Ovary Cell Unusual Amino Acid Human Peripheral Lymphocyte Polyamine Spermidine Lymphocyte Protein
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- 10.M.H. Park, S.I. Chung, H.L. Cooper, and J.E. Folk, The mammalian hypusine containing protein, eukaryotic initation factor 4D: Structural homology of this protein from several species, J. Biol. Chem. 259:4562 (1984).Google Scholar
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