Post-Translational Formation of Hypusine: Biogenesis of Translation Initiation Factor eIF-4D

  • Myung Hee Park
  • Alberto Abbruzzese
  • J. E. Folk
Part of the Advances in Experimental Medicine and Biology book series (NATO ASI F, volume 231)


Hypusine is a derivative of lysine which occurs in a single cellular protein, eIF-4D1. This unusual basic amino acid was discovered in 1971 in extracts of bovine brain by Shiba and coworkers, who determined its chemical structure as Nε-[4-amino-2-hydroxybutyl]lysine.2 Subsequently, its occurrence as the free amino acid and as a component of protein in various animal tissues was reported. Ten years later, we observed radiolabeling of a single protein (Mr; ~18,000, pI; ~5.3) in human peripheral lymphocytes following their incubation with [3H]spermidine and identified the radioactive component of this protein as hypusine.5 Based on this finding we commenced systematic studies of the hypusine-containing protein and of the mechanism and regulation of the biosynthesis of hypusine.


Chinese Hamster Ovary Cell Unusual Amino Acid Human Peripheral Lymphocyte Polyamine Spermidine Lymphocyte Protein 


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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Myung Hee Park
    • 1
  • Alberto Abbruzzese
    • 2
  • J. E. Folk
    • 1
  1. 1.National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA
  2. 2.Istituto di Chimica Biologica I Facoltá di MedicinaUniversitá di NapoliNapoliItaly

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