Post-Translational Modifications of the Elongation Factor 2
The elongation cycle in the course of protein biosynthesis is terminated by the translocation, which consists of a ribosome shift in the 5‵-3‵ direction along the mRNA, together with the transfer of the peptidyl-tRNA from the A to the P site and the release of the deacylated tRNA. The translocation is accompanied by the hydrolysis of a GTP molecule and is catalyzed, in the eukaryotic cells by a monomeric protein, the elongation factor 2 (EF-2), the molecular weight of which ranges between 90,000 and 110,000 in the sources so far examined (Bermek, 1978).
KeywordsElongation Factor Protein Biosynthesis Diphtheria Toxin FEBS Letter Beef Liver
Unable to display preview. Download preview PDF.
- Bermek, E., 1978, Mechanisms in polypeptide chain elongation on ribosomes, in: “Progress in Nucleic Acid Research and Molecular Biology,” W.E. Cohn ed., Academic Press, New York.Google Scholar
- Giovane, A., Servillo, L., Quagliuolo, L., and Balestrieri, C., 1987a, Purification of elongation factor 2 from human placenta and evidence of its fragmentation pattern in various eukaryotic sources, Biochem. J., 244:337.Google Scholar
- Giovane, A., Quagliuolo, L., Servillo, L., and Balestrieri, C., 1987b, Studies on the interaction between ribosome and elongation factor 2 by fluorescent labeling of the diphthamide residue, Exp. Biol. Med., in press.Google Scholar
- Kohno, K., Uchida, T., Ohkubo, H., Nakanishi, S., Nakanishi, T., Fukui, T., Ohtsuka, E., Ikehara, M., and Okada, Y., 1986, Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with the GTP-binding proteins, Proc. Natl. Acad. Sci. USA, 83:4978.PubMedCrossRefGoogle Scholar
- Moehring, J.M., and Moehring, T.J., 1984, Diphthamide: in vitro biosynthesis, in: “Methods in Enzymology,” 106:388.Google Scholar
- Uchida, T., 1982, in: “Molecular Action of Toxins and Viruses,” P. Cohen, and S. Van Heyningen, ed., Elsevier, Amsterdam.Google Scholar
- Van Ness, B.G., Howard, J.B., and Bodley, J.W., 1980a, ADP-ribosylation of elongation factor 2 by diphtheria toxin. Isolation and properties of the novel ribosyl-amino acid and its hydrolysis products, J. Biol. Chem., 255:10717.Google Scholar
- Van Ness, B.G., Howard, J.B., and Bodley, J.W., 1980b, ADP-ribosylation of elongation factor 2 by diphtheria toxin. NMR spectra and proposed structures of ribosyl-diphthamide and its hydrolysis products, J. Biol. Chem., 255:10710.Google Scholar