p34, A Protein Kinase Involved in Cell Cycle Regulation in Eukaryotic Cells

  • Giulio Draetta
  • Leonardo Brizuela
  • David Beach
Part of the Advances in Experimental Medicine and Biology book series (NATO ASI F, volume 231)


Investigation of the biochemical basis of cellular transformation is a major area of current research directed towards understanding the problem of tumorigenesis and human cancer. Much progress has been made in identifying primary oncogenic agents, both chemical and viral, in addition to the discovery of cellular oncogenes (Bishop, 1985). Biochemical activities have been associated with the products of some viral and cellular oncogenes, most notably those which belong to the protein kinase family (Erikson and Erikson, 1980).


Fission Yeast Cell Cycle Control Cell Division Cycle Yeast Cell Cycle Protein Kinase Family 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Beach, D. and Nurse, P. 1981, High-frequency transformation of the fission yeastSchizosaccharomyces pombe, Nature, 290:140–142.PubMedCrossRefGoogle Scholar
  2. Beach, D., Durkacz, B. and Nurse, P. 1982, Functionally homologous cell cycle control genes in budding and fission yeast. Nature, 300:706–709.PubMedCrossRefGoogle Scholar
  3. Bishop, J. M. 1985, Viral oncogenes, Cell 42:23–38.PubMedCrossRefGoogle Scholar
  4. Booher, R. and Beach D. 1986,. Site-specific mutagenesis of cdc2 +, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe, Molec. Cell. Biol. 6: 3523–3530.PubMedGoogle Scholar
  5. Costello, G., Rodgers, L. and Beach, D. 1986, Fission yeast enters the stationary phase GO state from either mitotic Gl or G2, Current Genetics 11: 119–125.CrossRefGoogle Scholar
  6. Draetta, G., Brizuela, L., Potashkin, J. and Beach, D. 1987, Identification of p34 and p13, human homologs of the cell cycle regulators of fission yeast encoded by cdc2 + and sucl +, Cell, in press.Google Scholar
  7. Ellis, R.W„ DeFeo, D., Shin, T.Y., Gonda, M.A, Young, HA., Tsuchida, N., Lowy, D.R and Scolnick, EM. 1981, The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes, Nature 292: 506–511.PubMedCrossRefGoogle Scholar
  8. Erikson, E. and Erikson, R.L. 1980, Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product. Cell 21:829–836.PubMedCrossRefGoogle Scholar
  9. Fukui, Y. and Kaziro, Y. 1985, Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe, EMBO J. 4: 687–691.PubMedGoogle Scholar
  10. Hartwell, L.H. 1974, Saccharomyces cerevisiae cell cycle, Bact. Rev. 38: 164–198.PubMedGoogle Scholar
  11. Hindley, J. and Phear, G A. 1984, Sequence of the cell division gene cdc2* from Schizosaccharomyces Pombe: patterns of splicing and homology to protein kinases, Gene 31: 129–134.PubMedCrossRefGoogle Scholar
  12. Hindley, J., Phear, G., Stein, M. and Beach, D. 1987, Sucl + encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces Pombe, Molec. Cell. Biol. 7: 504–511.PubMedGoogle Scholar
  13. Lorincz, A.T. and Reed, SX 1984, Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes, Nature 307:183–185.PubMedCrossRefGoogle Scholar
  14. Mao, J., Schaack, J., Sharp, S., Yamada, H., Kohli, J. and Soll, D. In Molecular genetics of yeast (D. Von Wettstein, ed.) 276–289 (1980).Google Scholar
  15. Mitchison, J.M. 1970, Physiological and Cytological Methods for S. pombe. In Methods in Cell Physiology Ed. Prescott, Vol. 4.Google Scholar
  16. Nurse, P. and Fantes, P. 1981, Cell Cycle controls in fission yeast: a genetic analysis, in P.C.L John Ed. The Cell Cycle, Cambridge 85–98.Google Scholar
  17. Nurse, P. and Thuriaux, P. 1980, Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe, Genetics 96: 627–637.PubMedGoogle Scholar
  18. Nurse, P. and Bissett, Y. 1981, gene required in Gl for committment to cell cycle and in G2 for control of mitosis in fission yeast. Nature 292: 558–560.PubMedCrossRefGoogle Scholar
  19. Powers, S., Kataoka, T., Fasano, O., Goldfarb, M., Strathern, J., Broach, J. and Wigler, M. 1984, Genes in Saccharomyces cerevisiae encode proteins with domains homologous to the mammalian ras proteins, Cell 36: 607–612.PubMedCrossRefGoogle Scholar
  20. Pringle, J.R. and Hartwell, L.H. 1981, The Saccharomyces cerevisîae cell cycle, in the Molecular Biology of the Yeast. (Strathern et al. eds.) Cold Spring Harbor, NY.Google Scholar
  21. Reed, S.J., Hadwiger, JA. and Lorincz, A.T. 1985, Protein kinase activity associated with the product of the yeast cell cycle gene CDC28, Proc. Natl. Acad. Sci. 82: 4055–4059.PubMedCrossRefGoogle Scholar
  22. Schechter, Y., Patchornik, A. and Burstein, Y. 1976, Selective chemical cleavage of tryptophenyl peptide bonds by oxidative chlorination with N-chlorosuccinimide, Biochemistry 15: 5071–5075.CrossRefGoogle Scholar
  23. Simanis, V. and Nurse, P. 1986, The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation, Cell 45: 261–268.PubMedCrossRefGoogle Scholar
  24. Studier, F.W. and Moffat, BA. 1986, Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes, J. Mol. Biol. 189: 113–130.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Giulio Draetta
    • 1
  • Leonardo Brizuela
    • 1
  • David Beach
    • 1
  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA

Personalised recommendations