Effect of Enzymatic Methylation on the Import of in Vitro Synthesized Apocytochrome C into Mitochondria

  • Woon Ki Paik
  • Kwang Sook Park
  • Blaise F. Frost
  • Sangduk Kim
Part of the Advances in Experimental Medicine and Biology book series (NATO ASI F, volume 231)


Little more than a dozen protein posttranslational side-chain modification reactions have been identified. One such reaction, protein methylation, occurs ubiquitously in nature, in organisms ranging from prokaryotic to eukaryotic. The reaction involves N-methylation of lysine, arginine, histidine, alanine, proline and glutamine, O-methylesterifi-cation of glutamic and aspartic acid, and S-methylation of cysteine and methionine (1–3).


Methyl Transferase Rabbit Reticulocyte Lysate Yeast Mitochondrion Protein Methylation Enzymatic Methylation 


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  1. 1.
    W. K. Paik and S. Kim, Protein methylation, Science, 172:114 (1971).CrossRefGoogle Scholar
  2. 2.
    W. K. Paik and S. Kim, Protein Methylation. Biochemistry: A Series of Monographs, Vol. 1, John Wiley & Sons, New York (1980).Google Scholar
  3. 3.
    W. K. Paik, and S. Kim, Protein methylation. In: The Enzymology of Post-Translational Modification of Proteins (Eds. R. B. Freedman and H. C. Hawkins), Academic Press, New York, Vol. 2 (1985), pp. 187–228.Google Scholar
  4. 4.
    W. K. Paik and S. Kim, Analysis of methylated amino acids. In: Pro-tein/Peptide Sequence Analysis: Current Methodology (Eds. A. S. Bhown), CRC Press (1987), in press.Google Scholar
  5. 5.
    W. K. Paik, and S. Kim, Protein methylase I. J. Biol. Chem., 243: 2108 (1968).PubMedGoogle Scholar
  6. 6.
    S. Kim and W. K. Paik, Purification and properties of protein methylase II, J. Biol. Chem., 245:1806 (1970).PubMedGoogle Scholar
  7. 7.
    W. K. Paik, and S. Kim, Solubilization and partial purification of protein methylase III from calf thymus nuclei, J. Biol. Chem., 245: 6010 (1970).PubMedGoogle Scholar
  8. 8.
    J. C. Wallwork, D. P. Quick and J. A. Duerre, Properties of soluble rat brain histone lysine methyl transferase, J. Biol. Chem., 252:5977 (1977).PubMedGoogle Scholar
  9. 9.
    E. Durban, S. Nochumson, S. Kim, W. K. Paik and S.-K. Chan, Cytochrome-specific protein-lysine methyl transferase from Neurospora crassa, J. Biol. Chem., 253:1427 (1978).PubMedGoogle Scholar
  10. 10.
    A. Sitaramayya, L. S. Wright and F. L. Siegel, Enzymatic methylation of calmodulin in rat brain cytosol, J. Biol. Chem., 255:8894 (1980).PubMedGoogle Scholar
  11. 11.
    H. W. Lee, S. Kim and W. K. Paik, S-Adenosy1methionine:protein-arginine methyltransferase. Purification and mechanism of the enzyme, Biochemistry, 16:78 (1977).PubMedCrossRefGoogle Scholar
  12. 12.
    G. S. Baldwin and P. R. Carnegie, Specific enzymatic methylation of an arginine in tire experimental allergic encephalomyelitis protein from human myelin, Science, 171:579 (1971).PubMedCrossRefGoogle Scholar
  13. 13.
    J. H. LaBadie, W. A. Dunn and N. N. Aronson, Hepatic synthesis of carnitine from protein-bound trimethyllysine, Biochem. J., 160:85 (1976).PubMedGoogle Scholar
  14. 14.
    S. J. Kleene, M. L. Toews and J. Adler, Isolation of glutamic acid methyl ester from an Escherichia coli, J. Biol. Chem., 252:3214 (1977).PubMedGoogle Scholar
  15. 15.
    P. Van Der Werf and D. E. Koshland, jr., Identification of y-glutamyl methyl ester in bacterial membrane protein involved in Chemotaxis, J. Biol. Chem., 252:2793 (1977).Google Scholar
  16. 16.
    M. Olsson and T. Lindahl, Repair of alkylated DNA in Escherichia coli, J. Biol. Chem., 255:10569 (1981).Google Scholar
  17. 17.
    W. K. Paik, E. Polastro and S. Kim, Cytochrome c methylation: Enzymology and biological significance, In: Current Topics in Cellular Google Scholar
  18. Regulation (Eds. B. L. Horecker and E. R. Stadtman), Academic Press, New York, Vol. 16 (1980), pp. 87–111.Google Scholar
  19. 18.
    W. K. Paik, J. Z. Farooqui and S. Kim, Protein methylation: Cytochrome c methylation as a model system, In: Advances in Enzyme Regulation (Eds. G. Weber), Pergamon Press, New York, Vol. 19 (1981), pp. 471–486.Google Scholar
  20. 19.
    W. K. Paik and S. Kim, Enzymology of protein methylation: Recent development, In: Biological Methylation and Drug Design (Eds. R. T. Borchardt, C. R. Creveling and P. M. Ueland), Humana Press, Clifton (1986), pp. 15–23.CrossRefGoogle Scholar
  21. 20.
    R. E. Dickerson, T. Takano, D. Eisenberg, O. B. Kallai, L. Samson, A. Cooper and E. Margoliash, Ferricytochrome c, J. Biol. Chem., 246: 1511 (1971).PubMedGoogle Scholar
  22. 21.
    T. Takano, O. B. Kallai, R. Swanson and R. E. Dickerson, The structure of ferrocvtochrome c at 2.45 A resolution, J. Biol. Chem., 248: (1973).Google Scholar
  23. 22.
    N. Tanaka, T. Yamane, T. Tsukihara, T. Ashida and M. Kakudo, The crystal structure of boni to (katsuo) ferrocytochrome c at 2.3 A resolution, J. Biochem. (Tokyo), 77:147 (1975).Google Scholar
  24. 23.
    M. O. Dayhoff, Atlas of Protein Sequence and Structure. Vol. 5 (1972), D-13 to D-24. National Biomedical Research Foundation.Google Scholar
  25. 24.
    R. E. Dickerson and R. Timkovich, In: Enzymes (Eds. P. Boyer), 3rd Ed., Vol. 11, Academic Press, New York (1975), pp. 397.Google Scholar
  26. 25.
    S. H. Speck, W. H. Koppenol, N. Osheroff, J. K. Dethmers, C. H. Kang, E. Margoliash and S. Ferguson-Miller, In: Membrane Bioenergetics (Eds. C. P. Lee, G. Schatz and L. Ernster), Addison-Wesley (1979), pp. 31–43.Google Scholar
  27. 26.
    S. Freguson-Miller, D. L. Brautigan and E. Margoliash, In: The Porphyrins (Eds. D. Dorphin), Academic Press (1979), pp. 149–241.CrossRefGoogle Scholar
  28. 27.
    R. J. DeLange, A. N. Glazer and E. L. Smith, Presence and location of an unusual amino acid, ε-N-trimethyllysine in cytochrome c of wheat germ and Neurospora, J. Biol. Chem., 244:1385 (1969).PubMedGoogle Scholar
  29. 28.
    R. J. DeLange, A. N. Glazer and E. L. Smith, Identification and location of e-N-trimethyllysine in yeast cytochrome c, J. Biol. Chem., 245:3325 (1969).Google Scholar
  30. 29.
    P. DiMaria, E. Polastro, R. J. DeLange, S. Kim and W. K. Paik, Studies on cytochrome c methylation in yeast, J. Biol. Chem., 254:4645 (1979).PubMedGoogle Scholar
  31. 30.
    P. DiMaria, S. Kim and W. K. Paik, Cytochrome c specific methylase from wheat germ, Biochemistry, 21:1036 (1982).PubMedCrossRefGoogle Scholar
  32. 31.
    J. Farooqui, S. Kim and W. K. Paik, In vitro studies on yeast cytochrome £ methylation in relation to protein synthesis, J. Biol. Chem. 255:4468 (1980).PubMedGoogle Scholar
  33. 32.
    M. Reporter, Protein synthesis in cultured muscle-cells methylation of nascent proteins, Arch. Biochem. Biophys., 158:577 (1973).CrossRefGoogle Scholar
  34. 33.
    J.-K. Chen and M. Liss, Evidence of carboxymethylation of nascent peptide chains on ribosomes, Biochem. Biophys. Res. Communs., 84: 261 (1978).CrossRefGoogle Scholar
  35. 34.
    S. Matsuura, M. Arpin, C. Hannum, E. Margoliash, D. D. Sabatini and T. Morimoto, In vitro synthesis and posttranslational uptake of cytochrome £ into isolated mitochondria- Role of a specific addressing signal in the apocytochrome £, Proc. Natl. Acad. Sci., USA, 78:4368 (1981).PubMedCrossRefGoogle Scholar
  36. 35.
    K. S. Park, B. F. Frost, m. Tuck, L. L. Ho, S. Kim and W. K. Paik, Enzymatic methylation of in vitro synthesized apo-cytochrome c enhances its transport into mitochondria, Submitted for publication.Google Scholar
  37. 36.
    J. M. Van Noort, B. Kraal, K. M. C. Sinjorgo, N. L. M. Persoon and E. S. D. Johanns, Methylation in vivo of elongation factor EF-Tu at lysine-56 decreases the rate of tRNA-dependent GTP hydrolysis, Eur. J. Biochem., 160:557 (1986).PubMedCrossRefGoogle Scholar
  38. 37.
    E. Polastro, A. G. Schneck, J. Leonis, S. Kim and W. K. Paik, Cytochrome c methylation, Intern. J. Biochem., 9:795 (1978).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Woon Ki Paik
    • 1
  • Kwang Sook Park
    • 1
  • Blaise F. Frost
    • 1
  • Sangduk Kim
    • 1
  1. 1.Fels Research InstituteTemple University School of MedicinePhiladelphiaUSA

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