Mammalian Protein Methylesterase
Posttranslational modification is an important event in protein structure and function. One of these modifications involves the transfer of methyl group from S-adenosyl-L-methionine to the free carboxyl groups of aspartyl and glutamyl side chains in proteins (Fig. 1). The enzyme involved in this reaction is protein-carboxyl methylase (PC.M; S-adenosylmethionine, protein-carboxyl 0-methyltransferase, EC 188.8.131.52). This ubiquitous enzyme esterifies free carboxyl groups of proteins resulting in the neutralization of their negative charges (Liss et al., 1969; Kim and Paik, 1970; Kim and Paik, 1971; Morin and Liss, 1973).
KeywordsLuteinizing Hormone Kidney Homogenate Methanol Formation Methyl Accept Chemotaxis Protein Carboxyl Methyl Transferase
sodium dodecyl sulfate
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