Mammalian Protein Methylesterase

  • Kannappan Veeraragavan
  • Claude Gagnon
Part of the Advances in Experimental Medicine and Biology book series (NATO ASI F, volume 231)

Abstract

Posttranslational modification is an important event in protein structure and function. One of these modifications involves the transfer of methyl group from S-adenosyl-L-methionine to the free carboxyl groups of aspartyl and glutamyl side chains in proteins (Fig. 1). The enzyme involved in this reaction is protein-carboxyl methylase (PC.M; S-adenosylmethionine, protein-carboxyl 0-methyltransferase, EC 2.1.1.24). This ubiquitous enzyme esterifies free carboxyl groups of proteins resulting in the neutralization of their negative charges (Liss et al., 1969; Kim and Paik, 1970; Kim and Paik, 1971; Morin and Liss, 1973).

Keywords

Hydrolysis Glycerol Adenosine Carboxyl Tryptophan 

Abbreviations used

PCM

protein-carboxyl methylase

PME

protein methylesterase

DTT

dithiothreitol

SDS

sodium dodecyl sulfate

PCMB

parachloromercuribenzoic acid

PMSF

phenylmethyl sulfonylf1uoride

ACTH

adrenocorticotropichormone

E-64

N-[N-(L-3-trans-carboxyoxi ran-2-carbonyl)-L-leucyl]-agmatine

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Kannappan Veeraragavan
    • 1
  • Claude Gagnon
    • 1
  1. 1.Urology Research Laboratory, Royal Victoria Hospital and Faculty of MedicineMcGill UniversityMontrealCanada

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