Macrophage Transglutaminases: Characterization of Molecular Species and Measurement of Enzymatic Modification by Cigarette Smoke Components
Transglutaminases (E.C. 188.8.131.52) are a family of calcium-dependent thiol enzymes which catalyze the covalent cross-linking of peptide-bound glutamine residues to a variety of primary amines, including the epsilonamino group of peptide-bound lysine residues (reviewed by Folk, 1980). This latter reaction represents a unique example of enzyme-catalyzed cross-linking, resulting in the formation of an epsilon-(gamma-glutamyl) lysine bond.
KeywordsCigarette Smoke Alveolar Macrophage Homologous Serum Smoke Exposure Factor Xiii
Unable to display preview. Download preview PDF.
- Birckbichler, P., Upchurch, H., Patterson, M., Orr, G., and Conway, E., 1985, Transglutaminase in the extracellular matrix of human diploid fibroblasts, Fed. Proc., 44:477Google Scholar
- Carp, H., and Janoff, A., 1978, Possible mechanisms of emphysema in smokers. In vitro suppression of serum elastase-inhibitory capacity by fresh cigarette smoke and its prevention by antioxidants, Am. Rev. Respir. Pis., 118:617.Google Scholar
- Cocuzzi, E., and Chung, S., 1985, Characterization of particulate-bound transglutaminase in rat lung, Fed. Proc., 44:1218.Google Scholar
- Guerin, M., 1980, A safe cigarette?, in “The Banbury Report”, Cold Spring Harbor Reports, New York.Google Scholar
- Murtaugh, M., and Davies, P., 1984, Transglutaminase expression and fibronectin cross-linking on the cell surface of peritoneal macrophages, J. Cell Biol., 99:326a.Google Scholar
- Roth, W., Chung, S., and Janoff, A., 1986, Inactivation of alveolar macrophage transglutaminase by oxidants in cigarette smoke, J. Leuk. Biol., 39:629.Google Scholar
- Roth, W., Fleit, H., Chung, S., and Janoff, A., 1987, Characterization of two distinct transglutaminases of murine bone marrow-derived macrophages: Effects of exposure of viable cells to cigarette smoke on enzyme activity, J. Leuk. Biol., 42(1) (in press).Google Scholar