Bilirubin in Afferent and Efferent Lymph in the Sheep

  • J. W. Quin
  • A. D. Shannon
  • F. C. Courtice

Abstract

Efferent (postnodal) lymph in the conscious sheep is characterised by a distinctive yellow colour which contrasts sharply with the almost colourless appearance of afferent (prenodal) lymph. The yellow colour of efferent lymph was analysed qualitatively and quantitatively by biochemical methods and was shown to be due to bilirubin (1). Qualitative analysis demonstrating this association included a positive indirect Van den Bergh reaction and a similar Rf value to bilirubin standards on thin-layer chromatography. Quantitative analysis of the bilirubin content of both plasma and lymph was carried out using a modification of the alkaline diazo-coupling method described by Brodersen and Jacobsen (2).

Keywords

Albumin Bilirubin 

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References

  1. 1.
    Quin, J.W., A.D. Shannon,: High concentration of bilirubin in post-nodal lymph associated with red blood cell catabolism in lymph nodes of the sheep. Lymphology (in press).Google Scholar
  2. 2.
    Brodersen, R., J. Jacobsen,; Separation and determination of bile pigments. Meth. biochem. Analysis 17 (1969) 31–54.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • J. W. Quin
    • 1
  • A. D. Shannon
    • 1
  • F. C. Courtice
    • 1
  1. 1.John Curtin School of Medical ResearchAustralian National UniversityCanberraAustralia

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