Morphometric Variability and Aberrations in Nematode Identification

  • Derek J. F. Brown
  • Brian Boag
Part of the Nato ASI Series book series (NSSA, volume 7)


Taxonomy, a term proposed by de Candolle (1813) for the theory of plant classification, is derived from the Greek words for “arrangement” and “law”, whereas systematics was evolved from the latinized Greek word “systema” and stems from its application to the system of classification by the early naturalists such as Linnaeus (Systema naturae, 1735). Today, the terms taxonomy and systematics are used interchangeably in nematology, often being misused simply to describe the process of determining specimen identity.


Nematode Species Morphometric Data Body Width Average Percentage Difference Scottish Crop Research Institute 
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  1. Allen, M.W. & Sher, S.A., 1967. Taxonomie problems concerning the phyto- parasitic nematodes. Ann. Rev. Phytopath., 5: 247–264.CrossRefGoogle Scholar
  2. Arpin, P., Jagers op Akkerhuis, G. & Pongé, J.-F., 1988. Morphometric variability in Clarkus papillatus (Bastian, 1865) Jairajpuri, 1970 in relation to humus type and season. Revue Nématol., 11: 149–158.Google Scholar
  3. Azmi, M.I. & Jairajpuri, M.S., 1978. Morphometric and allometric variations in the adults and juveniles of Helicotylenchus indicus Siddiqi, 1962. Indian J. Nematol., 6 (1976): 13–22.Google Scholar
  4. Bajaj, H.K. & Jairajpuri, M.S., 1977. Variability within Xiphinema insigne populations from India. Nematologica, 23: 33–46.CrossRefGoogle Scholar
  5. Barron, A.L.E., 1965. Using the microscope. London, Chapman & Hall, 257 p.Google Scholar
  6. Bird, G.W., Mai W.F., 1965. Morphometric and allometric variations of Trichodorus christiei. Nematologica, 13: 617–632.CrossRefGoogle Scholar
  7. Boag, B., 1981. The use of a digitising tablet to measure nematodes. Syst. Parasitol., 2: 145–147.CrossRefGoogle Scholar
  8. Boag, B., 1982. Effect of different concentrations of formalin and TAF on the fixation of Longidorus elongatus. Nematol. medit., 10: 115–118.Google Scholar
  9. Brown, D.J.F., 1981. The effect of operator and measuring system error on the morphometrical variability of one nematode specimen. Revue Nématol., 4: 169–171.Google Scholar
  10. Brown, D.J.F., 1983. The distribution of Longidoroidea (Nematoda) in Europe and variation in the morphology, biology and the virus transmission of Xiphinema diversicaudatum (Micoletzky, 1927) Thorne, 1939. Ph.D. Thesis. Open University, Milton Keynes, England 303 p.Google Scholar
  11. Brown, D.J.F., 1985. The effect, after four years, of a change in biotope on the morphometries of populations of Xiphinema diversicaudatum (Nematoda: Dorylaimoidea). Nematol. medit., 13: 7–13.Google Scholar
  12. Brown, D.J.F., 1986. Reproduction and inter-breeding within and between populations of Xiphinema divers icaudatum (Nematoda: Dorylaimoidea). Nematol. medit., 14: 73–82.Google Scholar
  13. Brown, D.J.F. & Boag, B., 1986. A study of the distribution and morphometrical variability between populations ofLongidorus vineacola Sturhan & Weischer, 1964 (Nematoda: Longidoridae). Revue Nématol., 9: 291.Google Scholar
  14. Brown, D.J.F., Luc, M. & Saka, V.W., 1983. Two new species of Xiphinema Cobb, 1913 (Nematoda: Dorylaimida) from Malawi, East Africa. Bull. Mus, natu. Hist, nat., Paris, 4e ser., 5 (A, 2): 521–529.Google Scholar
  15. Brown, D.J.F. & Topham, P.B., 1984. A comparison of reported variation in the morphometries of Xiphinema diversicaudatum (Nematoda: Dorylaimida) and the effects of some methods of preparing specimens for examination by optical microscopy. Nematol. medit., 12: 169–186.Google Scholar
  16. Brown, D.J.F. & Topham, P.B., 1985. Morphometric variability between populations of Xiphinema diversicaudatum (Nematoda: Dorylaimoidea). Revue Nématol., 8: 15–26.Google Scholar
  17. Candolle, A.P. de, 1813.Théorie élémentaire de la Botanique. Paris, viii + 500 + 27 pp.Google Scholar
  18. Courtney, W.D., Polley, D. & Miller, V.L., 1955. TAF, an improved fixative in nematode technique. PI. Dis. Reptr. 39, 570–571.Google Scholar
  19. Curran, J. & Hominick, W.M., 1981. Effect of mounting methods on taxonomie characters of adult male mermithids (Nematoda: Mermithidae). Nematologica, 26: 455–466.CrossRefGoogle Scholar
  20. Davide, R.G., 1980. Influence of different crops on the dimensions of Meloidogyne arenaria isolated from fig. Proc. helminth. Soc. Wash., 47: 80–84.Google Scholar
  21. De Grisse, A.T. & Loof, P.A.A., 1970. Intraspecific variation in some Criconematidae (Nematoda). Meded. Fac. LandbWetens. Gent, 35: 41–63.Google Scholar
  22. Debray, F. & Maupas, E., 1896. LeTylenchus devastatrix Kühn et la maladie vermiculaire des fèves en Algérie. L’Algérie agricole, 1–55.Google Scholar
  23. Ebsary, B.A., Potter, J.W. & Allen, W.A., 1984. Redescription and distribution of Xiphinema rivesi Dalmasso, 1969 and Xiphinema americanum Cobb, 1913 in Canada with a description ofXiphinema occiduum n. sp. (Nematoda: Longidoridae). Can. J. Zool., 62, 1696–1702.CrossRefGoogle Scholar
  24. Erbenova, M., 1975. [Ectoparasitic eelworms of the genus Xiphinema Cobb in the orchards of the CSR]. Sbomik UVTI-Zahradnictvi (Praha), 2: 79–86.Google Scholar
  25. Esser, R.P., 1974. Two permanent mounting methods compared after six years. Proc. Helminth, soc. Wash., 41: 10–13.Google Scholar
  26. Evans, K. & Franco, J., 1977. Morphological variation in some populations of potato cyst-nematodes from Europe and South America. Nematologica, 23: 417–430.CrossRefGoogle Scholar
  27. Fagerholm, H.P., 1979. Nematode length and preservatives, with a method for determining the length of live specimens. J. Parasitol., 65: 334–335.PubMedCrossRefGoogle Scholar
  28. Fisher, J.M., 1965. Studies in Paratylenchus nanus. I. Effects of variation in environment on several morphometric characters of adults. Nematologica, 11: 269–279.CrossRefGoogle Scholar
  29. Fortuner, R., 1979. Morphometrical variability in Helicotylenchus Steiner, 1945. I. The progeny of a single female. Revue Nématol., 2: 197–202.Google Scholar
  30. Fortuner, R., 1984. Statistics in taxonomie descriptions. Nematologica, 30: 187–192.CrossRefGoogle Scholar
  31. Fortuner, R., 1988. Variabitité et Identification des espèces chez les nématodes du genre Helicotylenchus. Paris, France, ORSTOM, Collection études et thèses, v + 232 p.Google Scholar
  32. Fortuner, R., Maggenti, A.R. & Whittaker, L.M., 1984. Morphometrical variability in Helicotylenchus Steiner, 1945. 4. Study of field populations of H, pseudorobustus and related species. Revue Nématol., 7: 121–135.Google Scholar
  33. Fortuner, R., Memy, G. & Rolax, C., 1981. Morphometrical variability in Helicotylenchus Steiner, 1945. 3. Observations on African populations of Helicotylenchus dihystera and considerations on related species. Revue Hématol., 4: 235–260.Google Scholar
  34. Fortuner, R. & Quénéhervé, P., 1980. Morphometrical variability in Helicotylenchus Steiner, 1945. 2. Influence of the host on H, dihystera (Cobb, 1893) Sher, 1961. levue Nématol., 3: 291–296.Google Scholar
  35. Franklin, M.T., 1970. Morphological variability and the species concept. Zesz. Probl. Postep, Nauk Roln., 92: 497–503.Google Scholar
  36. Frederick, J.J. & Tarjan, A.C., 1974. Morphological variation inXiphinema krugi Lordello, 1955. Proc, Soil Crop Sei, Soc, Florida, 34: 181–185.Google Scholar
  37. Frederick, J.J. & Tarjan, A.C., 1978. Variability in measurements made of same nematode specimen by various observers or by one observer on different days. Nematologica, 24: 476–478.CrossRefGoogle Scholar
  38. Goodey, J.B., 1952. The influence of the host on the dimensions of the plant-parasitic nematode Ditylenchus destructor, Ann, appl. Biol., 39: 468–474.CrossRefGoogle Scholar
  39. Goodey, J.B., 1959. Data to be considered, observed and, where possible, reported upon when presenting descriptions of new species. Nematologica, 4: 211–216.CrossRefGoogle Scholar
  40. Goodey, J.B., Peacock, F.C. & Pitcher, R.S., 1960. A redescription of Xiphinema diversicaudatum (Micoletzky, 1923 & 1927) Thorne, 1939 and observations on its larval stages. Nematologica, 5: 127–135.CrossRefGoogle Scholar
  41. Heyns, J., 1974a. The genus Xiphinema in South Africa. I. X. americanum- group (Nematoda: Dorylaimida). Phytophylactica, 6: 157–164.Google Scholar
  42. Heyns, J., 1974). The genus Xiphinema in South Africa. II. X. elongatum- group (Nematoda: Dorylaimida). Phytophylactica, 6: 249–260.Google Scholar
  43. Heyns, J., 1983. Problems of species delimitation in the genus Xiphinema with special reference to monosexual species. In: A.R. Stone, H.M. Piatt and L.F. Khalil (Eds), Concepts in Nematode Systematics, Systematics Association Special Volume No. 22. London and New York, Academic Press, 163–174.Google Scholar
  44. Hooper, D.J., 1969. Some problems in the systematics of soil nematodes. In: J.G. Sheals (ed.).The Soil Ecosystem, London, Systematics Association Publication No. 8: 131–142.Google Scholar
  45. Hooper, D.J., 1982. Observations on fixing, processing and mounting of nematodes. Nematologica, 28 (1981): 152.Google Scholar
  46. Hrzic, A., 1978. Prispevek k Poznavanju nematofaune virogradniske zemlji Slovenije(Xiphinema spp.). Zast. Bilja, 29: 387–396.Google Scholar
  47. Huxley, J.S. (Ed.) (1940). The New Systematics, Oxford, Clarendon Press, 583 p.Google Scholar
  48. Jairajpuri, M.S., 1969. Studies on Mononchida of India. I. The genera Hadronchus, lotonchus and Miconchus and a revised classification of Mononchida, New Order. Nematologica, 15: 557–581.CrossRefGoogle Scholar
  49. Khan, E., 1982. Inagreius gloriosus gen. n. sp n. and descriptions of three new species of Xiphinema Cobb, 1913 along with report on X. radicicola T. Goodey,1936 and X. elongatum Schuurmans Stekhoven & Teunissen, 1938 (Nematoda: Longidoroidea) from India. Indian J, Nematol., 11 (1981): 189–204.Google Scholar
  50. Kozlowska, J. & Seinhorst, J.W., 1979. Longidorus elongatus and closely related species in the Netherlands and Lower Saxony (Germany) with the description of two new species L. cylindricaudatus and L. intermedins (Nematoda: Dorylaimida). Nematologica, 25: 42–53.CrossRefGoogle Scholar
  51. Lamberti, F. & Bleve-Zacheo, T., 1979. Studies on Xiphinema americanum sensu lato with descriptions of fifteen new species (Nematoda: Longidoridae). Nematol. medit, 7: 51–106.Google Scholar
  52. Lamberti, F. & Golden, A.M., 1984. Redescription of Xiphinema americanum Cobb, 1913 with comments on its morphometric variations. J.Nematol., 16: 204–206.PubMedGoogle Scholar
  53. Lamberti, F. & Sher, S.A., 1969. A comparison of preparation techniques in taxonomie studies of Longidorus africanus Merny. J. Nematol., 1: 193–200.PubMedGoogle Scholar
  54. Lima, M.B., 1965. Studies on species of the genus Xiphinema and other nematodes. Ph.D. Thesis. University of London, 165 p.Google Scholar
  55. Little, M.D., 1966. Comparative morphology of six species of Strongyloides (Nematoda) and redefinition of the genus. J.Parasitol., 52: 69–84.PubMedCrossRefGoogle Scholar
  56. Loof, P.A.A. & Maas, P.W. Th., 1972. The genus Xiphinema (Dorylaimida) in Surinam. Nematologica, 18: 92–119.CrossRefGoogle Scholar
  57. Luc, M., Maggenti, A.R., Fortuner, R., Raski, D.J. & Geraert, E., 1987. A reappraisal of Tylenchina (Nemata). 1. For a new approach to the taxonomy of Tylenchina. Revue Nématol., 10: 127–134.Google Scholar
  58. Luc, M., Loof, P.A.A. & Brown, D.J.F., 1984. On the systematics of eleven Xiphinema species (Nematoda: Longidoridae) described from India. Revue Nématol., 7: 399–405.Google Scholar
  59. Luc, M. & Southey, J.F., 1980. Study of biometrical variability in Xiphinema insigne Loos, 1949, and X. elongatum Schuurmans Stekhoven & Teunissen, 1938; description of X. savanicola n. sp. (Nematoda: Longidoridae) and comments on thelytokous species. Revue Nématol., 3: 243–269.Google Scholar
  60. Maggenti, A.R., 1983. Nematode higher classification as influenced by species and family concepts. In: A.R. Stone, H.M. Piatt and L.F. Khalil (Eds), Concepts in Nematode Systematics, Systematics Association Special Volume No. 22. London and New York, Academic Press: 25–40.Google Scholar
  61. Maggenti, A.R. & Viglierchio, D.R., 1965. Preparation of nematodes for microscope study - perfusion by vapor phase in killing and fixing. Hilgardia, 36: 435–463.Google Scholar
  62. Martelli, G.P., Cohn, E. & Dalmasso, A., 1966. A redescription ofXiphinema italiae Meyl, 1953 and its relationship to Xiphinema arenarium Luc et Dalmasso, 1963 andXiphinema conurum Siddiqi, 1964. Hematological 12: 183–194.Google Scholar
  63. Martelli, G.P. & Lamberti, F., 1967. Le specie di Xiphinema Cobb, 1913 trovate in Italia e commenti sulla presenza di Xiphinema americanum Cobb. (Nematoda: Dorylaimoidea). Phytopath. Medit., 6: 65–85.Google Scholar
  64. Mayr, E., Linsley, E.G. & Usinger, R.L., 1953. Methods and principles of systematic zoology. New York and London, McGraw-Hill, 336 p.Google Scholar
  65. Micoletzky, H., 1927. 3. Neue und seltene freilebende Nematoden aus den Wolgagebeit (Kama). Zool. Anz., 73: 113–123.Google Scholar
  66. Noble, B., 1964. Numerical methods: 1. Interaction, programming and algebraic equations. Oliver & Boyd, Edinburgh, 156 p.Google Scholar
  67. Premvati, F, 1958. Studies on Strongyloides of primates. 1. Morphology and life history of Strongyloides fulleorni von Lis tow, 105. Can. J. Zool., 36: 65–77.CrossRefGoogle Scholar
  68. Rohde, R.A. & Jenkins, W.R., 1957. Effect of temperature on the life cycle of stubby-root nematodes. Phytopathology, 47: 29.Google Scholar
  69. Saxena, P.K., Chhabra, M.K. & Joshi, R., 1973. Xiphinema neoamericanum sp.n. (Nematoda: Longidoridae) from India. Zool. Anz., 191: 130–132.Google Scholar
  70. Scott, J.A., 1929. The length of specimens of the dog hookworm after various methods of fixation. J.Parasitol., 16: 54–55.CrossRefGoogle Scholar
  71. Sharma, R.K. & Saxena, V., 1981. Two new species of the genus Xiphinema Cobb, 1913 (Dorylaimida: Nematoda). Ind. J. Parasitol., 5: 95–99.Google Scholar
  72. Snedecor, G.W. & Cochran, W.G., 1980. Statistical Methods (7th edition). Iowa St. Univ. Press, Ames, xvi + 507 p.Google Scholar
  73. Soleim, O., 1976. A comparison of Thynnascaris adunca populations in two different cod populations. Norw. J. Zool., 24: 319–323.Google Scholar
  74. Stone, A.R., 1971. Effect of processing on measurements of Heterodera rostochiensis larvae. Nematologica, 17: 167–171.CrossRefGoogle Scholar
  75. Sturhan, D., 1963. Der pflanzenparasitische Nematode Longidorus maximus, seine Biologie und Ökologie, mit Untersuchungen an L. elongatus und Xiphinema diversicaudatum. Z. Angew. Zool., 50: 129–193.Google Scholar
  76. Szczygiel, A., 1974. Plant parasitic nematodes associated with strawberry plantations in Poland. Zesz. Probl. Postep. Nauk Roln., 154: 1–132.Google Scholar
  77. Tarjan, A.C., 1964. Two new American dagger nematodes (Xiphinema: Dorylaimidae) associated with citrus, with comments on the variability of X. bakeri Williams, 1961. Proc. helminth. Sac. Wash., 31: 65–76.Google Scholar
  78. Tarjan, A.C., 1969. Variation within the Xiphinema americanum group (Nematoda: Longidoridae). Nematologica, 15: 241–252.CrossRefGoogle Scholar
  79. Tarjan, A.C. & Frederick, J.J., 1978. Intraspecific morphological variation among populations of Pratylenchus brachyurus and P. coffeae. J. Nematol., 10: 152–160.Google Scholar
  80. Tarte, R. & Mai, W.F., 1976. Morphological variation in Pratylenchus penetrans. J. Nematol., 18: 185–195.Google Scholar
  81. Teploukhova, T.N., 1974. [Some data on the nematode vectors of phyto- pathogenic viruses in the USSR.] Zashch. Rast., 41: 40–45.Google Scholar
  82. Terlidou, M.C., 1967. [Nematodes attacking grapevines and their control.] Vine Inst. Lykovryssi. Kiphissia Greece, 106 p.Google Scholar
  83. Weischer, B., 1964. International Symposium on Xiphinema. hematology News, 6: 1–6.Google Scholar
  84. Woitowicz, M.R., Golden, A.M., Forer, L.B. & Stouffer, R.F., 1982. Morphological comparison between Xiphinema rivesi Dalmasso and X. americanum populations from the Eastern United States. J.Nematol., 14: 511–516.Google Scholar
  85. Yeates, G.W., 1972. Constancy of specific soil nematode dimensions with depth and time. Nematologica, 18: 418.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Derek J. F. Brown
    • 1
  • Brian Boag
    • 1
  1. 1.Zoology DepartmentScottish Crop Research InstituteInvergowrie, DundeeScotland, UK

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