Adenine Metabolism and Nucleic Acid Synthesis: Applications to Microbiological Oceanography

  • David M. Karl
  • Christopher D. Winn
Part of the NATO Conference Series book series (NATOCS, volume 15)


Marine microbial ecology is currently one of the least developed areas of microbiological research. This situation has been, in part, due to the limited availability of methods for evaluating the in situ rates of metabolism and growth of naturally occurring microbial populations. In fact, it may be fair to state that our present understanding of the integrated functioning of marine ecosystems is methods limited. A period of rapid advance in our understanding of microbiological oceanographic processes following the successful development and application of each new experimental approach, is evidence of this limitation.


Salvage Pathway Nucleic Acid Synthesis Northeast Pacific Ocean Nucleic Acid Precursor Cellular Nucleotide 
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  1. Brock, T. D. 1967. Bacterial growth rates in the sea: Direct analysis by thymidine autoradiography. Science 155: 81–83.ADSCrossRefGoogle Scholar
  2. Eppley, R. W. 1981. Relations between nutrient assimilation and growth in phytoplankton with a brief review of estimates of growth rate in the ocean, pp. 251–263. In: TS. Piatt [ed.]. Physiological bases of phytoplankton ecology. Can. Bull. Fish. Aquat. Sci. 210: 346 p.Google Scholar
  3. Eppley, R. W., R. W. Holmes, and J. D. H. Strickland. 1967. Sinking rates of marine phytoplankton measured with a fluorometer. J. Exp. Mar. Biol. Ecol. 1: 191–208.CrossRefGoogle Scholar
  4. Fellows, D., D. M. Karl, and G. Knauer. 1981. Vertical distribution, production and sedimentation of adenosine triphosphate in the upper 1550 meters of the Northeast Pacific Ocean. Deep-Sea Res. 28A: 921–936.CrossRefGoogle Scholar
  5. Ferguson, R. L., and P. Rublee. 1976. Contribution of bacteria to standing crop of coastal plankton. Limnol. Oceanogr. 21: 141–145.CrossRefGoogle Scholar
  6. Fuhrman, J. A., and F. Azam. 1980. Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl. Environ. Microbiol. 39: 1085–1095.Google Scholar
  7. Fuhrman, J. A., and F, Azam. 1982. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: evaluation and field results. Mar. Biol. 66: 109–120.CrossRefGoogle Scholar
  8. Robbie, J. E., R. J. Daley, and S. Jasper. 1977. Use of Nuclepore filters for counting bcteria by fluorescence microscopy. Appl. Environ. Microbiol. 33: 1225–1228.Google Scholar
  9. Hochstadt, J. 1974. The role of the membrane in the utilization of nucleic acid precursors. CRC Crit. Rev. Biochem. 2: 259–310.CrossRefGoogle Scholar
  10. Holm-Hansen, O. 1969, Algae: Amounts of DNA and organic carbon in single cells. Science 163: 87–88.ADSCrossRefGoogle Scholar
  11. Holm-Hansen, O., W. H. Sutcliffe Jr., and J. Sharp. 1968. Measurement of deoxyribonucleic acid in the ocean and its ecological significance. Limnol. Oceanogr. 13: 507–514.CrossRefGoogle Scholar
  12. Karl, D. M. 1979. Measurement of microbial activity and growth in the ocean by rates of stable ribonucleic acid synthesis. Appl. Environ. Microbiol. 38: 850–860.Google Scholar
  13. Karl, D. M. 1980. Cellular nucleotide measurements and applications in microbial ecology. Microbiol. Rev. 44: 739–796.Google Scholar
  14. Karl, D. M. 1981. Simultaneous rates of ribonucleic acid and deoxyribonucleic acid syntheses for estimating growth and cell division of aquatic microbial communities. Appl. Environ. Microbiol. 42: 802–810.Google Scholar
  15. Karl, D. M. 1982. Selected nucleic acid precursors in studies of aquatic microbial ecology. Appl. Environ. Microbiol. 44: 891–902.Google Scholar
  16. Karl, D. M., and G. A. Knauer. 1983. Vertical distribution, transport and exchange of carbon in the Northeast Pacific Ocean: Evidence for multiple zones of bacterial activity. Deep-Sea Res. In press.Google Scholar
  17. Karl, D. M., C. D. Winn, and D. C. L. Wong. 1981a. RNA synthesis as a measure of microbial growth in aquatic environments. I. Evaluation, verification and optimization of methods. Mar. Biol. 64: 1–12.CrossRefGoogle Scholar
  18. Karl, D. M., C. D. Winn, and D. C. L. Wong. 1981b. RNA synthesis as a measure of microbial growth in aquatic environments. II. Field applications. Mar. Biol. 64: 13–21.CrossRefGoogle Scholar
  19. Kjeldgaard, N. O. 1967. Regulation of nucleic acid and protein synthesis in bacteria. Adv. Microb. Physiol. 1: 39–95.CrossRefGoogle Scholar
  20. Maaloe, O., and N. O. Kjeldgaard. 1966. Control of Macromolecular Synthesis. W. A. Benjamin Inc., New York.Google Scholar
  21. Mandalstam, J., and K. McQuillen. 1976. Biochemistry of Bacterial Growth. John Wiley and Sons, New York.Google Scholar
  22. Meyer-Reil, L.-A. 1978. Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl. Environ. Microbiol. 36: 506–512.Google Scholar
  23. Moriarty, D. J. W., and P. C. Pollard. 1981. DNA snythesis as a measure of bacteria productivity in seagrass sediments. Mar. Ecol. Prog. Ser. 5: 151–156.CrossRefGoogle Scholar
  24. Moriarty, D. J. W., and P. C. Pollard. 1982. Diel variations in bacteria productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA. Mar. Biol. 72: 165–173.CrossRefGoogle Scholar
  25. Nierlich, D. P. 1978. Regulation of bacterial growth, RNA and protein samthesis. Ann. Rev. Microbiol. 32: 393–432.CrossRefGoogle Scholar
  26. Sutcliffe, W. J., Jr., R. W. Sheldon, and A. Prakash. 1970. Certain aspects of production and standing stock of particulate matter in the surface waters of the Northwest Atlantic Ocean. J. Fish. Res. Board Can. 27: 1917–1926.CrossRefGoogle Scholar
  27. Thelander, L., and P. Reichard. 1979. Reduction of ribonucleotides. Ann. Rev. Biochem. 48: 133–158.CrossRefGoogle Scholar
  28. Tobin, R. S., and D. H. J. Anthony. 1978. Tritiated thymidine incorporation as a measure of microbial activity in lake sediments. Limnol. Oceanogr. 23: 161–165.CrossRefGoogle Scholar
  29. Winn, C. D., and D. M. Karl. 1983. Microbial productivity and growth rate estimates in the tropical North Pacific Ocean. Biol. Oceanogr. in press.Google Scholar
  30. Yamazaki, H., and K. Leung. 1981. Determination of the total rates of synthesis and degradation of RNA in bacterial cultures. Can. J. Microbiol. 27: 168–174.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • David M. Karl
    • 1
  • Christopher D. Winn
    • 1
  1. 1.Department of OceanographyUniversity of HawaiiHonoluluUSA

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