Abstract
Marine microbial ecology is currently one of the least developed areas of microbiological research. This situation has been, in part, due to the limited availability of methods for evaluating the in situ rates of metabolism and growth of naturally occurring microbial populations. In fact, it may be fair to state that our present understanding of the integrated functioning of marine ecosystems is methods limited. A period of rapid advance in our understanding of microbiological oceanographic processes following the successful development and application of each new experimental approach, is evidence of this limitation.
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References
Brock, T. D. 1967. Bacterial growth rates in the sea: Direct analysis by thymidine autoradiography. Science 155: 81–83.
Eppley, R. W. 1981. Relations between nutrient assimilation and growth in phytoplankton with a brief review of estimates of growth rate in the ocean, pp. 251–263. In: TS. Piatt [ed.]. Physiological bases of phytoplankton ecology. Can. Bull. Fish. Aquat. Sci. 210: 346 p.
Eppley, R. W., R. W. Holmes, and J. D. H. Strickland. 1967. Sinking rates of marine phytoplankton measured with a fluorometer. J. Exp. Mar. Biol. Ecol. 1: 191–208.
Fellows, D., D. M. Karl, and G. Knauer. 1981. Vertical distribution, production and sedimentation of adenosine triphosphate in the upper 1550 meters of the Northeast Pacific Ocean. Deep-Sea Res. 28A: 921–936.
Ferguson, R. L., and P. Rublee. 1976. Contribution of bacteria to standing crop of coastal plankton. Limnol. Oceanogr. 21: 141–145.
Fuhrman, J. A., and F. Azam. 1980. Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl. Environ. Microbiol. 39: 1085–1095.
Fuhrman, J. A., and F, Azam. 1982. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: evaluation and field results. Mar. Biol. 66: 109–120.
Robbie, J. E., R. J. Daley, and S. Jasper. 1977. Use of Nuclepore filters for counting bcteria by fluorescence microscopy. Appl. Environ. Microbiol. 33: 1225–1228.
Hochstadt, J. 1974. The role of the membrane in the utilization of nucleic acid precursors. CRC Crit. Rev. Biochem. 2: 259–310.
Holm-Hansen, O. 1969, Algae: Amounts of DNA and organic carbon in single cells. Science 163: 87–88.
Holm-Hansen, O., W. H. Sutcliffe Jr., and J. Sharp. 1968. Measurement of deoxyribonucleic acid in the ocean and its ecological significance. Limnol. Oceanogr. 13: 507–514.
Karl, D. M. 1979. Measurement of microbial activity and growth in the ocean by rates of stable ribonucleic acid synthesis. Appl. Environ. Microbiol. 38: 850–860.
Karl, D. M. 1980. Cellular nucleotide measurements and applications in microbial ecology. Microbiol. Rev. 44: 739–796.
Karl, D. M. 1981. Simultaneous rates of ribonucleic acid and deoxyribonucleic acid syntheses for estimating growth and cell division of aquatic microbial communities. Appl. Environ. Microbiol. 42: 802–810.
Karl, D. M. 1982. Selected nucleic acid precursors in studies of aquatic microbial ecology. Appl. Environ. Microbiol. 44: 891–902.
Karl, D. M., and G. A. Knauer. 1983. Vertical distribution, transport and exchange of carbon in the Northeast Pacific Ocean: Evidence for multiple zones of bacterial activity. Deep-Sea Res. In press.
Karl, D. M., C. D. Winn, and D. C. L. Wong. 1981a. RNA synthesis as a measure of microbial growth in aquatic environments. I. Evaluation, verification and optimization of methods. Mar. Biol. 64: 1–12.
Karl, D. M., C. D. Winn, and D. C. L. Wong. 1981b. RNA synthesis as a measure of microbial growth in aquatic environments. II. Field applications. Mar. Biol. 64: 13–21.
Kjeldgaard, N. O. 1967. Regulation of nucleic acid and protein synthesis in bacteria. Adv. Microb. Physiol. 1: 39–95.
Maaloe, O., and N. O. Kjeldgaard. 1966. Control of Macromolecular Synthesis. W. A. Benjamin Inc., New York.
Mandalstam, J., and K. McQuillen. 1976. Biochemistry of Bacterial Growth. John Wiley and Sons, New York.
Meyer-Reil, L.-A. 1978. Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl. Environ. Microbiol. 36: 506–512.
Moriarty, D. J. W., and P. C. Pollard. 1981. DNA snythesis as a measure of bacteria productivity in seagrass sediments. Mar. Ecol. Prog. Ser. 5: 151–156.
Moriarty, D. J. W., and P. C. Pollard. 1982. Diel variations in bacteria productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA. Mar. Biol. 72: 165–173.
Nierlich, D. P. 1978. Regulation of bacterial growth, RNA and protein samthesis. Ann. Rev. Microbiol. 32: 393–432.
Sutcliffe, W. J., Jr., R. W. Sheldon, and A. Prakash. 1970. Certain aspects of production and standing stock of particulate matter in the surface waters of the Northwest Atlantic Ocean. J. Fish. Res. Board Can. 27: 1917–1926.
Thelander, L., and P. Reichard. 1979. Reduction of ribonucleotides. Ann. Rev. Biochem. 48: 133–158.
Tobin, R. S., and D. H. J. Anthony. 1978. Tritiated thymidine incorporation as a measure of microbial activity in lake sediments. Limnol. Oceanogr. 23: 161–165.
Winn, C. D., and D. M. Karl. 1983. Microbial productivity and growth rate estimates in the tropical North Pacific Ocean. Biol. Oceanogr. in press.
Yamazaki, H., and K. Leung. 1981. Determination of the total rates of synthesis and degradation of RNA in bacterial cultures. Can. J. Microbiol. 27: 168–174.
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Karl, D.M., Winn, C.D. (1984). Adenine Metabolism and Nucleic Acid Synthesis: Applications to Microbiological Oceanography. In: Hobbie, J.E., Williams, P.J.l. (eds) Heterotrophic Activity in the Sea. NATO Conference Series, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9010-7_9
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DOI: https://doi.org/10.1007/978-1-4684-9010-7_9
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