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Regulation and Function of Plasmid Encoded Virulence Determinants of Yersinia

  • H. Wolf-Watz
  • A. Forsberg
  • R. Rosqvist
  • I. Bölin
  • K. Erickson
  • L. Norlander
  • M. Rimpilainen
  • T. Bergman
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 51)

Abstract

The three virulent members of the genus Yersinia harbour related virulence plasmids with a molecular weight of about 60–70 kb (1). When exponential phase cultures of these organisms growing in a Ca2+ free medium are shifted from 26*C to 37*C, growth ceases over a period of about 2 generations (2, 3, 4). If however 2.5 mM Ca2+ is present in the medium growth continues normally. These bacteria are referred to as being Ca2+ dependent (CD). Plasmid cured strains, however, do not show this dependency on Ca2+ and are thus, Ca2+ independent (CI). Such bacteria are always avirulent. By transposon insertion mutagenesis a 20 kb region of the virulence plasmid has been identified which is involved in this low calcium response (Jcr). Such Cl-mutants do not require Ca2+ for prolonged growth at 37*C and they are not virulent. Although the plasmids of Y. enterocolitica, Y. pestis and Y. pseudotuberculosis have been subjected to rearrangements, the Ca2+ region, however, of the different plasmids is conserved (Fig.1) (1).

Keywords

Temperature Sensitive Temperature Sensitive Mutant Virulence Plasmid High Transcriptional Activity PstI Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • H. Wolf-Watz
    • 1
    • 2
  • A. Forsberg
    • 1
    • 2
  • R. Rosqvist
    • 1
    • 2
  • I. Bölin
    • 1
    • 2
  • K. Erickson
    • 1
    • 2
  • L. Norlander
    • 1
    • 2
  • M. Rimpilainen
    • 1
    • 2
  • T. Bergman
    • 1
    • 2
  1. 1.Department of MicrobiologyUniversity of UmeåUmeåSweden
  2. 2.Department of Cell and MicrobiologyUmeåSweden

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