Recombinant Genetic Approaches for Efficient Ethanol Production

  • H. W. Stokes
  • S. K. Picataggio
  • D. E. Eveleigh


Ethanol has potential use as an alternative liquid transportation fuel and as a chemical feedstock. The recent restrictions on the availability of oil focused attention on ethanol production through the microbial fermentation of biomass. The economic incentives to develop such fermentation processes are borderline. This paper discusses the manner in which recombinant genetic approaches may potentially relieve certain of the economic bottlenecks and put the fermentative production of ethanol on a firmer basis. It then reviews the genetics of bacteria and yeasts. The manner in which these processes may be used is illustrated with protocols for the development of new microbial strains that can utilize a range of less expensive substrates (cellulose and starch) and that are more tolerant of high ethanol concentrations.


Ethanol Production Pyruvate Decarboxylase High Ethanol Concentration Hybrid Plasmid Fuel Alcohol 


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Copyright information

© American Solar Energy Society, Inc. 1983

Authors and Affiliations

  • H. W. Stokes
    • 1
  • S. K. Picataggio
    • 1
  • D. E. Eveleigh
    • 1
  1. 1.Department of Biochemistry and Microbiology, Cook College, New Jersey Agricultural Experiment StationRutgers UniversityNew BrunswickUSA

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