Skip to main content

Recombinant Genetic Approaches for Efficient Ethanol Production

  • Chapter
Advances in Solar Energy

Abstract

Ethanol has potential use as an alternative liquid transportation fuel and as a chemical feedstock. The recent restrictions on the availability of oil focused attention on ethanol production through the microbial fermentation of biomass. The economic incentives to develop such fermentation processes are borderline. This paper discusses the manner in which recombinant genetic approaches may potentially relieve certain of the economic bottlenecks and put the fermentative production of ethanol on a firmer basis. It then reviews the genetics of bacteria and yeasts. The manner in which these processes may be used is illustrated with protocols for the development of new microbial strains that can utilize a range of less expensive substrates (cellulose and starch) and that are more tolerant of high ethanol concentrations.

Harold (Hatch) W. Stokes is currently a Research Associate at the University of Connecticut. Born and educated in Australia, he received his Ph.D. from Monash University in Microbial Genetics (1980). He explored the genetics of Zymomonas while a Research Associate at Cook College, Rutgers University.

Stephen Picataggio obtained his B.S. degree (1975) from Wagner College and then worked at Best Foods, N.J. He subsequently enrolled as a Doctoral Candidate in the Graduate Microbiology Program at Rutgers University.

Douglas E. Eveleigh is a Professor of Microbiology at Rutgers University. Trained in Britain he has worked in Canada and the U.S. on various topics in applied microbiology.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. A H. Rose. 1977. In Economic microbiology: Alcoholic beverages. Vol. 1. Edited by A. H. Rose. New York: Academic Press, pp. 1–44.

    Google Scholar 

  2. J. Bulock. 1978. In Microbial technology: Current state, future prospects. Twenty-ninth Symposium of the Society for General Microbiology, U.K. Edited by A. T. Bull, D. C. Eliwood and C. Ratledge. Cambridge: Cambridge University Press, pp. 309–325.

    Google Scholar 

  3. N. Kosaric; D. C. M. Ng; I. Russell; and G. S. Stewart. 1980. Adv. Appl. Microbiol. 26:147–227.

    Google Scholar 

  4. D. M. Munnecke. 1981. In Bio mass conversion processes for energy and fuels. Edited by S. S. Sofer and O. R. Zaborsky. New York: Plenum, pp. 339–355.

    Google Scholar 

  5. R. C. Righelato. 1980. Phil. Trans. R. Soc. Lond. B 290:303–312.

    Google Scholar 

  6. K. Venkatasubramanian and C. R. Keim. 1981. Ann. New York Acad. Sei. 369:187–204.

    Google Scholar 

  7. National Alcohol Fuels Commission. 1981. Fuel alcohol, an alternative for the 1980s. Final report GPO. Washington, D.C.

    Google Scholar 

  8. R. S. Chambers; R. A. Herendeen; J. J. Joyce; and P. S. Penner. 1979. Science 206:789–795.

    Google Scholar 

  9. D. Brandt. 1981. In Biomass conversion processes for energy and fuels. Edited by S. S. Sofer and O. R. Zaborsky. New York: Plenum, pp. 357–373.

    Google Scholar 

  10. H. W. Doelle. 1915. Bacterial metabolism. 2ded. New York: Academic Press.

    Google Scholar 

  11. G. Gottschalk. 1978. Bacterial metabolism. 1st ed. New York: Springer-Verlag.

    Google Scholar 

  12. A. Margaritis and C. R. Wilke. 1978.Biotech. Bioeng. 20:709.

    Google Scholar 

  13. K. J. Lee; M. Lefebvre; D. E. Tribe; and P. L. Rogers. 1980. Biotech. Letters 2:487–492.

    Google Scholar 

  14. R. Maleszka; I. A. Veliky; and H. Schneider. 1981. Biotech. Letters 3:415–420.

    Google Scholar 

  15. I. G. Prince and J. P. Barford. 1982.Biotech. Letters. 4:263–268.

    Google Scholar 

  16. M. L. Skotnicki; K. J. Lee; D. E. Tribe; and P. L. Rogers. 1982. “Genetic engineering of microorganisms for chemicals.” Basic Life Sciences 19:271–290.

    Google Scholar 

  17. Alcon Biotechnology Ltd. 1982. Portsmouth, U.K. Brochure.

    Google Scholar 

  18. T. K. Ghose and R. D. Tyagi. 1979.Biotech. Bioeng. 21:1387–1400.

    Google Scholar 

  19. P. L. Rogers; K. J. Lee; M. L. Skotnicki; and D. E. Tribe. 1981. In Advances in biotechnology. Vol. 2. Edited by M. Moo-Young and C. W. Robinson. Toronto: Pergamon Press, pp. 189–194.

    Google Scholar 

  20. M. Wada; J. Kato; and I. Chibata. 1981. Europ. J. Appl. Microbiol. Biotechnol. 11:67–71.

    Google Scholar 

  21. Y-Y. Linko; H. Jalanka; and P. Linko. 1981. Biotech. Letters 3:263–268.

    Google Scholar 

  22. W. Grote; K. J. Lee; and P. L. Rogers. 1980. Biotech. Letters 2:481–486.

    Google Scholar 

  23. E. J. Arcuri; R. M. Worden; and S. E. Shumate II. 1980. Biotech. Letters 2:499–504.

    Google Scholar 

  24. A. Margaritis; P. K. Bajpai; and J. B. Wallace. 1981. Biotech. Letters 3:613–618.

    Google Scholar 

  25. G. Amin and H. Verachten. 1982. Europ. J. Appl. Microbiol. Biotechnol. 14:59–63.

    Google Scholar 

  26. A. Ramalingham and R. K. Finn. 1977.Biotechnol. Bioeng. 19:583–589.

    Google Scholar 

  27. B. Maiorell and C. R. Wilke. 1980.Biotechnol. Bioeng. 22:1749–1751.

    Google Scholar 

  28. J. H. Lee; J. C. Woodward; R. J. Pagan; and P. L. Rogers. 1981. Biotech. Letters 3:177–182.

    Google Scholar 

  29. M. Bacila and J. Horii. 1979. TIBS. 4:59–61.

    Google Scholar 

  30. G. Moulin; H. Boze; and P. Galzy. 1982. J. Ferment. Technol. 60:25–29.

    Google Scholar 

  31. H. W. Doelle. 1982. Europ. J. Appl. Microbiol. Biotechnol. 14:241–246.

    Google Scholar 

  32. S. Cromie and H. W. DoeUe. 1982.Europ. J. Appl. Microbiol. Biotechnol. 14:69–73.

    Google Scholar 

  33. S. S. Dills; A. Apperson; M. R. Schmidt; and M. H. Daier, Jr. 1980. Microbiol. Revs. 44:385–418.

    Google Scholar 

  34. A. H. Romano; J. D. Trifone; and M. Brustolon. 1979. J. Bacteriol. 139:9–97.

    Google Scholar 

  35. H. W. Doelle. 1972.Biochim. Biophys. Acta. 258:404.

    Google Scholar 

  36. D. I. C. Wang; I. Biocic; H. S. Fang; and J. D. Wang. 1979. Proceedings of the Third Annual Biomass Energy Systems Conference. Springfield. Va.: National Technical Information Service, and personal communication.

    Google Scholar 

  37. J. G. Zeikus and T. K. Ng. 1982. Ann. Reports Ferment. Processes 5:263–289.

    Google Scholar 

  38. H. Schneider; P. Y. Wang; Y. K. Chan; and R. Maleska. 1981. Biotech. Letters 3:89–92.

    Google Scholar 

  39. P. J. Slininger; R. J. Bothast; J. E. Van Cauwen- berg; and C. P. Kurzman. 1982.Biotech. Bioeng. 24:371–384.

    Google Scholar 

  40. T. W. Jeffries, 1981. Biotech. Letters 3:213–218.

    Google Scholar 

  41. C. S. Gong; L. D. McCracken; and G. T. Tsao. 1981. Biotech. Letters 3:24–50.

    Google Scholar 

  42. R. Maleszka; P. Y. Yang; and H. Schneider. 1982. Can. J. Biochem. 60:144–151.

    Google Scholar 

  43. R. W. Armentrout and R. D. Brown. 1981. Appl Environment. Microbiol. 41:1355–1362.

    Google Scholar 

  44. D. J. Whittle; D. G. Kilbum; R. A. J. Warren; and R. C. Miller, Jr. 1982. Gene 17:139–145.

    Google Scholar 

  45. H.J.J. Van Vuuren and L. Meyer, 1982. Biotech. Letters 4:253–256.

    Google Scholar 

  46. K. Holland. 1980. Unpublished observations.

    Google Scholar 

  47. S. W. Brown and S. G. Oliver. 1982. Biotech. Letters 4:269-274.

    Google Scholar 

  48. L. G. Ljundahl; F. Bryant; L. Carreira; T. Saki; and J. Weigel. 1981. Trends in the biology offermentation for fuels and chemicals. Edited by A. Hollander. New York: Plenum Press.

    Google Scholar 

  49. S. Aiba; M. Shoda; and M. Nagatani. 1968. Biotech. Bioeng. 10:845–864.

    Google Scholar 

  50. S. L. Chen. 1981. Biotech. Bioeng. 23:1827–1836.

    Google Scholar 

  51. S. Hayashida; D. D. Feng; and M. Hongo. 1974. Agric. Biol. Chem. 38:2001–2006.

    Google Scholar 

  52. J. Burrell. 1981. Unpublished observations.

    Google Scholar 

  53. T. W. Nagodawithana and K. H. Steinkraus. 1916. Appl. Microbiol. 31:158–162.

    Google Scholar 

  54. J. M. Navarro and G. Durand. 1978. Ann. Microbiol. Inst. Pasteur. 129B:215–225.

    Google Scholar 

  55. D. S. Thomas and A. H. Rose. Arch. Mikrobiol. 1979. 122:49–55.

    Google Scholar 

  56. G. K. Hoppe and G. S. Hansford, 1982. Biotech. Letters 4:39–44.

    Google Scholar 

  57. S. W. Brown; S. G. Oliver; D. E. F. Harrison; and R. C. Righelato. 1981. Europ. J. Appl. Microbiol. Biotechnol. 11:151–155.

    Google Scholar 

  58. L. O. Ingram. 1982. Seminar.

    Google Scholar 

  59. M. Ciriacy. 1975. Mutation Res. 29:315–326.

    Google Scholar 

  60. C. Wills; P. Kratofil; D. Londo; and T. Martin. 1981. Arch. Biochem. Biophys. 210:775–785.

    Google Scholar 

  61. V. M. Williamson; E. T. Young; and M. Ciriacy. 1981. Cell 23:605–614.

    Google Scholar 

  62. O. T. Avery; C. M. McLeod; and M. McCarty. 1944. J. Exp. Med. 79:137–158.

    Google Scholar 

  63. P. E. Young and J. Spizizen. 1961. J. Bact. 81:823–829.

    Google Scholar 

  64. S. N. Cohen; A. C. Y. Chang; and L. Hsu. 1972. Proc. Nat. Acad. Sei. (USA) 69:2210–2214.

    Google Scholar 

  65. M. J. Sinclair and A. F. Morgan. 1978. Aust. J. Biol. Sei. 31:769–688.

    Google Scholar 

  66. M. Susskind and D. Botstein. 1978.Microbiol. Rev. 42:385.

    Google Scholar 

  67. N. D. Zinder and J. Lederberg. 1952. J. Bact. 64:619.

    Google Scholar 

  68. M. L. Morse; E. M. Lederberg; and J. Lederberg. 1956.Genetics 41:142.

    Google Scholar 

  69. B. W. Holloway. 1969. Bact. Rev. 33:419–443.

    Google Scholar 

  70. E. M. Lederberg and S. N. Cohen. 1974. J. Bact. 119:1072.

    Google Scholar 

  71. J. Swings and J. DeLey. 1911. Bact. Rev. 41:1–46.

    Google Scholar 

  72. H. W. Stokes and E. L. Dally. 1982. Unpublished observation.

    Google Scholar 

  73. F. Jacob and E. L. Wollman. 1961. Sexuality and the Genetics of Bacteria. New York: Academic Press.

    Google Scholar 

  74. P. M. Chandler and V. Krishnapillai. 1974. Genet. Res. 23:239–250.

    Google Scholar 

  75. D. Haas and B. W. Holloway. 1976.Molec. Gen. Genet. 144:243–251.

    Google Scholar 

  76. B. W. Holloway. 1979. Plasmid 2:1–19.

    Google Scholar 

  77. M. L. Skotnicki; D. E. Tribe: and P. L. Rogers. 1980. Appl. Env. Microbiol. 40:7–12.

    Google Scholar 

  78. P. Starlinger. 1980. Plasmid 3:241–259.

    Google Scholar 

  79. N. Kleckner; J. Roth; and D. Botstein. 1977. J. Mol. Biol. 116:125–159.

    Google Scholar 

  80. S. Harayama; T. Masataka; and T. Lino. 1981. Mol. Gen. Genet. 184:52–55.

    Google Scholar 

  81. E. L. Dally. 1982. M. S. Thesis. Rutgers University.

    Google Scholar 

  82. S. Baumberg; G. Cornelius; M. Panagiotakopoulos; and M. Roberts. 1980. J. Gen. Microbiol. 119:257–262.

    Google Scholar 

  83. M. Sato; B. J. Staskamicz; N. J. Panopoulos; S. Peters; and M. Honma. 1981. Plasmid 6:325–331.

    Google Scholar 

  84. R. L. Sinsheimer. Ann. Rev. Biochem. 1977. 46: 415–438.

    Google Scholar 

  85. H. V. Bernard and D. R. Helinski. 1980. Genetic engineering. Vol 2. New York: Plenum Press, pp. 133–167.

    Google Scholar 

  86. G. Ditta; S. Stanfield; D. Corbin; and D. R. Helinski. 1980. Proc. Natl. Acad. Sei. (USA) 77:7347–7351.

    Google Scholar 

  87. E. L. Dally; H. W. Stokes; and D. E. Eveleigh. 1982. Biotech. Letters 4:91–96.

    Google Scholar 

  88. H. W. Stokes and E. L. Dally. 1982. Unpublished observations.

    Google Scholar 

  89. H. W. Stokes; E. L. Dally; R. L. Wüliams; B. S. Montenecourt; and D. E. Eveleigh. 1980. Chemistry in energy production. A.C.S. Symp. pp. 115–121.

    Google Scholar 

  90. L. E. Bryan and S. Kwan. 1981. J. Antimicrob. Chemother. 8 Suppl. D:l-8.

    Google Scholar 

  91. M. Bagdasarian; R. Lurz; B. Ruckert; F. C. H. Franklin; M. M. Bagdasarian; J. Frey; and K. N. Timmis. 1981. Gene 16:237–247.

    Google Scholar 

  92. T. D. Petes. 1980.Ann. Rev. Biochem. 49:845–876.

    Google Scholar 

  93. L. H. Hartwell. 1914. Bacteriol. Rev. 38:164–198.

    Google Scholar 

  94. M. Guerineau. 1979. In Viruses and plasmids in fungi, Edited P. A. Lemke. New York: Marcel Dekker, pp. 155–181.

    Google Scholar 

  95. The molecular biology of the yeast Saccharo- myces. 1981. J. N. Strathern; E. W. Jones; J. R. Broach, eds. Cold Spring Harbor Laboratory.

    Google Scholar 

  96. J. Lodder. 1970. In The yeasts: A taxonomie study. New York: Interscience.

    Google Scholar 

  97. J. Herskowitz and J. Oshima. 1981. In The molecular biology of the yeast Saccharomyces. J. Strathem; E. W. Jones; J. R. Broach, eds. Cold Spring Harbor Laboratory, pp. 181–210.

    Google Scholar 

  98. E. Bücking-Throm; W. Duntze; L. H. Hartwell; and J. R. Manney. 1973. Expl. Cell Res. 76:99–110.

    Google Scholar 

  99. E. Bücking-Throm and W. Duntze. 1970. J. Bacteriol. 104:1388–1390.

    Google Scholar 

  100. R. K. Mortimer and D. Schild. 1981. In The molecular biology of the Yeast Saccharomyces. J. Strathem; E. W. Jones; J. R. Broach, eds. Cold Spring Harbor Laboratory, pp. 11–26.

    Google Scholar 

  101. R. K. Mortimer and D. Schild. Microbiol. Rev. 1980. 44:519–537.

    Google Scholar 

  102. J. N. Strathem; C. S. Newton; J. Herskowitz; and J. B. Hicks. 1979. Cell 18:309–315.

    Google Scholar 

  103. R. Bigilas; J. Keesey; and T. R. Ferik. 1977. JCN-UCLA Symp. 8:179–189.

    Google Scholar 

  104. D. H. Clayton; T. A. Howard; and P. A. Martin. 1972. Amer. Soc. Brew. Chem. Proc. 30:78–81.

    Google Scholar 

  105. R. S. Tubb. 1979.J. Inst. Brew. 85:286–289.

    Google Scholar 

  106. P. van Solnigen and J. B. van der Platt. 1977. J. Bacteriol. 130:946–947.

    Google Scholar 

  107. J. Russell and G. G. Stewart. 1979. J. Inst. Brew. 85:95–98.

    Google Scholar 

  108. R. B. Wickner. 1981. In The molecular biology of the yeast Saccharomyces. J. N. Strathem; E. W. Jones; J. R. Broach, eds. Cold Spring Harbor Laboratory, pp. 415–444.

    Google Scholar 

  109. B. Dujon. In The molecular biology of the yeast Saccharomyces. 1981. J. N. Strathem; E. W. Jones; J. R. Broach, eds. Cold Spring Harbor Laboratory, pp. 505–635.

    Google Scholar 

  110. J. H. Sinclair; B. J. Stephens; P. Sanghavi; and M. Rabinowitz. 1967. Science 156:1234–1237.

    Google Scholar 

  111. C. P. Hollenberg; P. Borst; and E. F. J. van Bmggen. 1970. Biochem. Biophys. Acta. 209:1–15.

    Google Scholar 

  112. G. D. Clark-Walker and G. G. Miklos. 1974. Eur. J. Biochem. 41:359–365.

    Google Scholar 

  113. J. R. Broach. 1982.Cell 28:203–204.

    Google Scholar 

  114. S. C. Falco; Y. Li; J. R. Broach; and D. Botstein. 1977. All. 29:573–584.

    Google Scholar 

  115. D. M. Livingston. 1977.Genetics 86:73–84.

    Google Scholar 

  116. G. G. Stewart; J. Russell; C. J. Panchal. 1980. Abstr. VI in Int. Symp. on Yeasts. London (Ontario), Canada, pp. 212–221.

    Google Scholar 

  117. A. Hinnen; J. B. Hicks; and G. R. Fink. 1978. Proc. Natl. Acad. Sei. (USA) 75:1929–1933.

    Google Scholar 

  118. J. D. Beggs. 1978.Nature 275:104–109.

    Google Scholar 

  119. K. Stmhl; D. J. Stinchcomb; S. Scherer; and R. W. Davis. 1979. Proc. Nat. Acad. Sei. (USA) 76:1035–1039.

    Google Scholar 

  120. L. Clark and J. Carbon. 1980. Nature 287:504–509.

    Google Scholar 

  121. D. Morris; J. Noti; F. K. Osbome; and A. Szalay. 1981. DNA 1:27–35.

    Google Scholar 

  122. D. Morris. Personal Communication.

    Google Scholar 

  123. C. Segen; P. J. Farabraugh; A. Hinnen; J. M. Walsh; G. R. Fink. 1979. In Genetic engineering principles and methods. Vol 1. J. K. Setlow and A. Hollander, eds. New York: Plenum Press, pp. 117–132.

    Google Scholar 

  124. D. Botstein; S. C. Falco; S. E. Stewart; M. Bren- nan; S. Scherer; D. T. Stinchcomb; K. Stmhl; and R. W. Davis. 1979.Gene 8:17–24.

    Google Scholar 

  125. F. Bolivar; R. Rodriquez; P. J. Greene; M. C. Betlack; H. Heyneker; J. H. Cresa; S. Falfon; and H. W. Boyer. 1977. Gene 2:95–113.

    Google Scholar 

  126. M. L. Bach; F. Lacrante; and D. Botstein. 1979. Proc. Natl. Acad. Sei. (USA) 76:386–390.

    Google Scholar 

  127. Methods in enzymology. Vol. 68. 1979. Edited by R. Wu. New York: Academic Press.

    Google Scholar 

  128. G. Hohn. In Methods in enzymology. Vol. 68. 1979. Edited by R. Wu. New York: Academic Press, pp. 299–308.

    Google Scholar 

  129. J. Collins. In Methods in enzymology. Vol. 68. 1979. Edited by R. Wu. New York: Academic Press, pp. 309L-325.

    Google Scholar 

  130. M. Gmnstein and D. S. Hogness. 1975. Proc. Natl. Acad. Sei. (USA) 72:3961–3965.

    Google Scholar 

  131. R. A. Kramer; J. R. Cameron; and R. W. Davis. 1976.Cell 8:227–232.

    Google Scholar 

  132. J. D. Petes; J. R. Broach; P. Wensink; L. M. Hereford; G. R. Fink; and D. Botstein. 1978. Gene 4:37–49.

    Google Scholar 

  133. M. V. Olson; B. D. Hall; J. R. Cameron; and R. W. Davis. 1979. J. Molec. Biol. 127:285–295.

    Google Scholar 

  134. D. L. Montgomery; B. D. Hall; S. Gillam; and M. S. Smith. 1978. Cell 14:673–680.

    Google Scholar 

  135. K. Stmhl and R. W. Davis. 1977. Proc. Natl. Acad. Sei. (USA) 74:5255–5259.

    Google Scholar 

  136. B. Ratzkin and J. Carbon. 1977. Proc. Natl. Acad. Sei. (USA) 74:487–491.

    Google Scholar 

  137. D. Vapnek. 1977. Proc. Natl. Acad. Sei. (USA) 74:3508–3512.

    Google Scholar 

  138. K. Stmhl; J. R. Cameron; and R. W. Davis. 1976. Proc. Natl. Acad. Sei. (USA) 74:1471–1475.

    Google Scholar 

  139. L. Clarke and J. Carbon. 1978. J. Mot. Biol. 120: 517–532.

    Google Scholar 

  140. R. C. Dickson and J. S. Markin. 1978.Cell 15: 123–130.

    Google Scholar 

  141. M. V. Olson. 1918. In Genetic engineering principles and methods. Vol. 3. J. K. Setlow and A. Hollander, eds. New York: Academic Press, pp. 57–88.

    Google Scholar 

  142. A. Hinnen; P. J. Farabraugh; C. Ilgen; and G. R. Fink. 1979. In Eukaryotic gene regulation. Vol. 14. R. Axel; T. Maniatis; M. Fox, eds. New York: Academic Press, pp. 43–51.

    Google Scholar 

  143. K. A. Nasmyth and S. S. Reed. 1980. Proc. Nad. Acad. Sei. (USA) 77:2119–2123.

    Google Scholar 

  144. K. A. Nasmyth and K. Thatchell. 1980.Cell 19: 753–764.

    Google Scholar 

  145. V. M. Williamson; J. Bennetzen; E. J. Young; K. Nasmyth; and B. D. Hall. 1980.Nature 283:214–216.

    Google Scholar 

  146. R. W. Davis; G. R. Fink; and D. Botstein. 1981. Ann. Rev. Biochem. 50:112–158.

    Google Scholar 

  147. J. J. Pantkier; P. Foumier; H. Heslot; and A. Ramback. 1980. Curr. Genetics 2:109–113.

    Google Scholar 

  148. A. Jiminez and J. Davies. 1980. Nature 280: 869–871.

    Google Scholar 

  149. J. D. Beggs; J. van den Berg; A. van Ooyen; and C. Weissman. 1980. Nature 283:835–840.

    Google Scholar 

  150. R. C. Dickson. 1980.Gene 10:347–356.

    Google Scholar 

  151. O. Mercereau-Puijalen; F. Lacroute; and P. Kou- rilsky. 1980.Gene 11:163–167.

    Google Scholar 

  152. R. A. Hitzeman; F. E. Hagie; H. L. Levine; D. V. Goeddel; G. Ammerer; and B. D. Hall. 1981. Nature 293:717–722.

    Google Scholar 

  153. C. C. Emeis. 1971. Amer. Soc. Brew. Chem. Prac. 29:58–62.

    Google Scholar 

  154. H. Tamaki. 1978. Molecular Gen. Genet. 164: 205–209.

    Google Scholar 

  155. F. K. Zimmerman; N. A. Khan; and N. R. Eaton. 1973. Molec. Gen. Genet. 123:29–41.

    Google Scholar 

  156. D.B. Mowshowitz. 1979. J. Bact. 137:1200–1207.

    Google Scholar 

  157. G. G. Stewart. Personal Communication.

    Google Scholar 

  158. G. B. Calleja; S. L. Rick; A. Nasim; C. V. Lusena; C. C. Champagne; I. A. Veliky; and F. Morannelli. 1982. In The XIII Proceedings of the International Congress of Microbiology. Boston, Mass.; August 8–13, 1982.

    Google Scholar 

  159. K. Yamane and B. Maruo. 1980. In Molecular breeding and genetics of applied microorganisms. Edited by K. Sakaguchi and M. Okanish, Tokyo, Japan: Kodansha-Academic Press, pp. 117–123.

    Google Scholar 

  160. J. A. Bassham. 1975. Biotech. Bioeng. Symposium. No. 5. Sept. 19, 1975.

    Google Scholar 

  161. C. S. Gong and G. T. Tsao. 1979. Ann. Repisms. Ferment. Processes 3:111–140.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1983 American Solar Energy Society, Inc.

About this chapter

Cite this chapter

Stokes, H.W., Picataggio, S.K., Eveleigh, D.E. (1983). Recombinant Genetic Approaches for Efficient Ethanol Production. In: Böer, K.W., Duffie, J.A. (eds) Advances in Solar Energy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8992-7_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-8992-7_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8994-1

  • Online ISBN: 978-1-4684-8992-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics