Effect of Different Urinary Constituents on Inhibiting or Accelerating Calcium Oxalate Crystallization
Sutor1 has described a method for assessing the effect of different compounds on the inhibition or acceleration of crystallization of calcium oxalate. This method was qualitative and did not give sufficiently reproducible values. The assay procedure was made quantitative by measuring calcium and considerably improved to give more reproducible values (details to be communicated elsewhere). Preliminary trials using acetate and phosphate buffers showed that the inhibitory effects of some compounds were dependent on the type of buffer used. Since acetate buffer is unphysiological it was felt that more useful and reliable information could be obtained if our improved method was applied using a phosphate buffer.
KeywordsUric Acid Aspartic Acid Glucuronic Acid Pyruvic Acid Calcium Oxalate
Unable to display preview. Download preview PDF.
- 4.L. H. Smith, W. C. Thomas, Jr., and C. D. Arnaud, in: “Urinary Calculi,” L. Cifuentes-Delatte, A. Rapado, and A. Hodgkinson, eds., Karger, Basel (1973).Google Scholar
- 5.B. E. C. Nordin, H. Barry, L. Bulusu, and R. Speed, in: “Urinary Calculi,” L. Cifuentes-Delatte, A. Rapado, and A. Hodgkinson, eds., Karger, Basel (1973).Google Scholar
- 8.R. N. Class and A. H. Smith, J. Biol. Chem. 151:363 (1943).Google Scholar
- 10.W. C. Thomas, M. H. Malagodi, and O. M. Rennert, in: “Proceedings Fourth International Urolithiasis Research Symposium,” L. H. Smith, B. Finlayson, and W. G. Robertson, eds., Plenum Press, New York (in press).Google Scholar