The Ultrastructure of Rat Renal Tubules in Experimental Calcium Oxalate Nephrolithiasis
Formation of calcium oxalate crystals in the rat kidney was induced by a lithogenic challenge made by intraperitoneal injection of various amounts of sodium oxalate utilizing 0.22M sodium oxalate solution in 0.9% saline. The rat kidneys were fixed by retrograde aortic perfusion with a glutaraldehyde-paraformaldehyde mixture at different time intervals following the lithogenic challenge. The kidneys were sliced into small pieces and processed for transmission and scanning electron microscopy. Normal rat kidneys were fixed and used as controls. Two major problems were encountered in the ultrastructural study of renal tubules containing calcium oxalate crystals. First, the small number of crystals in the renal tissue of animals injected with the lesser doses of sodium oxalate made systemic study difficult. Second, crystals could not be infiltrated with plastic and caused sectioning problems for transmission electron microscopy. This problem was solved by treating the crystals with hydrochloric acid or EDTA, which resulted in their partial digestion facilitating plastic infiltration.
KeywordsProximal Tubule Calcium Oxalate Calcium Oxalate Crystal Tubular Lumen Sodium Oxalate
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