Decomposition of Exogenous 14C-Oxalate (14C-OX) to 14C-Carbon Dioxide (14CO2) In Vitro and in Animals
The question concerning bacterial decomposition of oxalate by intestinal microorganisms is unresolved. Varying amounts of oxalate were incubated with human feces. The formed CO2 was determined by liquid scintillation counting. Oxalate was found to be metabolized by bacteria from the human feces. However, the deterioration was very small (0.15%). In a second experiment rats and guinea pigs received 14C-Ox by gastric intubation. The exhalation14CO2 of measured. In guinea pigs the major pathway of metabolism of exogenous Ox was found to be respiratory exhalation (72%). In contrary, the rat excreted only 5% as CO2. Therefore, balance studies with Ox in guinea pigs are only valuable when following the CO2 exhalation.
KeywordsLiquid Scintillation Counting Balance Study Human Feces Oxalate Sodium Gastric Intubation
Unable to display preview. Download preview PDF.