The Effect of Urine and Other Inhibitors on the Growth and Aggregation of Calcium Oxalate Crystals in Vitro
Crystal growth and aggregation are generally regarded as important steps in the formation of renal stones, but no previous study has examined the effects of urine or its components on these two processes by measuring them as separate phenomena. Using a modification1 of the Coulter Counter technique2 it has been possible to measure crystal growth and aggregation independently even when they are occurring simultaneously. In this study we have quantified the effect of various inhibitors found in urine over a range of concentrations spanning those which would be anticipated in urine diluted to 1%. These results have then been compared with the mean level of inhibition of growth and of aggregation observed for 66 urine specimens (1% v/v) from normal males in an attempt to identify those urinary constituents most likely to be responsible for that inhibition.
KeywordsCrystal Growth Zeta Potential Chondroitin Sulphate Sodium Salt Calcium Oxalate
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- 1.R. L. Ryall and V. R. Marshall, in: “Urinary Calculi, Proceedingsof the International Urinary Stone Conference, 1979,” J. G. Brockis and B. Finlayson, eds., PSG Publication Co., Inc., Littleton, MA (1980) (in press).Google Scholar
- 3.R. L. Ryall and V. R. Marshall, in: “Proceedings Fourth Inter national Urolithiasis Research Symposium,” L. H. Smith, B. Finlayson and W. G. Robertson, eds. Plenum Press, New York (in press).Google Scholar
- 8.W. G. Robertson, F. Knowles, and M. Peacock, in: “Urolithiasis Research,” H. Fleisch, W. G. Robertson, L. H. Smith, and W. Vahlensieck, eds. Plenum Press, New York/London (1976).Google Scholar