Advertisement

Studies on the Mechanism of Lectin-Dependent T Cell-Mediated Cytolysis: Use of Lens Culinaris Hemagglutinin A to Define the Role of Lectin

  • William R. Green
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 146)

Abstract

Alloimmune cytotoxic T cell populations demonstrate exquisite immunologic specificity, killing only target cells which display alloantigens related to those used for immunization (1,2). It was noted some time ago, however, that in the presence of the plant lectins concanavalin A (Con A) and phythohemagglutinin such specificity is not maintained; rather a variety of target cells, including those syngeneic to the effector cell source, are lysed (3–5).

Keywords

Target Cell Effector Cell None None Specific Lysis Cell Mitogen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Brunner, K.T., and J.C. Cerottini. Cell-mediated cytotoxicity, allograft rejection and tumor immunity. Adv. Immunol. 18:67 (1974)PubMedCrossRefGoogle Scholar
  2. 2.
    Wagner, H.S., S.W. Harris, and M. Feldman. Cell-mediated immune response vitro. II. The role of thymus and thymus- derived lymphocytes. Cell. Immunol. 4:39. (1972)PubMedCrossRefGoogle Scholar
  3. 3.
    Moller, E. Contact-induced cytotoxicity by lymphoid cells containing foreign isoantigens. Science 147:873. (1964)CrossRefGoogle Scholar
  4. 4.
    Forman J., and G. Moller. Generation of cytotoxic lymphocytes in mixed lymphocyte reactions. I. Specificity of the effector cells. J. Exp. Med. 138:672. (1973)PubMedCrossRefGoogle Scholar
  5. 5.
    Bevan M.H., and M. Cohn. Cytotoxic effects of antigen and mitogen-induced T cells on various targets. J. Immunol. 114:559. (1975)PubMedGoogle Scholar
  6. 6.
    Küppers, R.C., and C.S. Henney. Evidence for direct linkage between antigen recognition and lytic expression in effector T cells. J. Exp. Med. 143:684. (1976)PubMedCrossRefGoogle Scholar
  7. 7.
    Küppers, R.C., and C.S. Henney. Studies on the mechanism of lymphocyte-mediated cytolysis. IX. Relationships between antigen recognition and lytic expression in killer T cells. J. Immunol. 118:71. (1977)PubMedGoogle Scholar
  8. 8.
    Fishelson, Z., and G. Berke. T lymphocyte-mediated cytolysis: Dissociation of the binding and lytic mechanisms of the effector cell. J. Immunol. 120:1121. (1978)PubMedGoogle Scholar
  9. 9.
    Greeen, W.R., Z.K. Ballas, and C.S. Henney. Studies on the mechanism of lymphocyte-medited cytolysis. XI. The role of lectin in lectin-dependent cell-mediated cytotoxicity. J. Immunol. 121:1566. (1978)Google Scholar
  10. 10.
    Parker, W.L., and E. Martz. Lectin-induced nonlethal adhesions between cytolytic T Drmphocytes and antigenically unrecognizable tiimor cells and nonspecific “triggering” of cytolysis. J. Immunol. 125:25. (1980)Google Scholar
  11. 11.
    Gately, M.K., and E. Martz. Comparative studies on the mechanisms of nonspecific. Con A-dependent cytolysis and specific T cell-mediated cytolysis. J. Immunol. 119:1711. (1977)PubMedGoogle Scholar
  12. 12.
    Berke G., V. Hu, E. McVey, and W.R. Clark. T lymphocyte- mediated cytolysis. I. A common mechanism for target recognition in specific and lectin-dependent cytolysis. J. Immunol. 127:776. (1981)PubMedGoogle Scholar
  13. 13.
    Berke, G., E. McVey, V. Hu, and W.R. Clark. T lymphocyte- mediated cytolysis. II. Role of target cell histocompatibility antigens in recognition and lysis. J. Immunol. 127:782. (1981)PubMedGoogle Scholar
  14. 14.
    Bonavida, B., and T.P. Bradley. Studies on the induction and expression of T cell-mediated immunity. V. Lectin-induced nonspecific cell-mediated cytotoxicity by alloimmune lymphocytes. Transplantation 21:94. (1976)PubMedCrossRefGoogle Scholar
  15. 15.
    Rubens, R.P., and C.S. Henney. Studies on the mechanism of lymphocyte-mediated cytolysis. VIII. The use of Con A to delineate a distinctive killer T cell subpopulation. J. Immunol. 118:180. (1977)PubMedGoogle Scholar
  16. 16.
    Kohier, G., I. Lefkovits, B. Elliott, and A. Coutinho. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7:758. (1977)CrossRefGoogle Scholar
  17. 17.
    Nabholz, M., M. Cianfriglia, O. Acuto, A. Conzelman, W. Haas, H.V. Böhmer, H.R. MacDonald, and J.P. Johnson. Cytolytically active murine T cell hybrids. Nature 287:437. (1980)PubMedCrossRefGoogle Scholar
  18. 18.
    Kaufmann, Y., G. Berke, and Z. Eshhar. Cytotoxic T Ijmiphocyte hybridomas that mediate specific tumor-cell lysis Jha vitro. Proc. Natl. Acad. Sci. 78:2502. (1981)PubMedCrossRefGoogle Scholar
  19. 19.
    Gensheimer G.G., and J.R. Neefe. Cell-mediated lympholysis: A receptor-associated lytic mechanism. Cell. Immunol. 36:54. (1978)PubMedCrossRefGoogle Scholar
  20. 20.
    Todd, R. Functional characterization of membrane components of cytotoxic peritoneal exudate T lympocytes. II. Trypsin sensitivity of the killer cell receptor. Transplantation 20:314. (1975)PubMedCrossRefGoogle Scholar
  21. 21.
    Küppers, R.C., Z.K. Ballas, W.R. Green, and C.S. Henney. Quantitative appraisal of H-2 products in T cell-mediated lysis by allogeneic and syngeneic effector cells. J. Immunol. 127:500. (1981)PubMedGoogle Scholar
  22. 22.
    Ballas, Z.K, W.R. Green, and C.S. Henney. Studies on the mechanism of T cell-mediated lysis. XIII. Lectin-dependent T cell-mediated cytotoxicity is supported by Con A-coupled Sepharose beads. Cell. Immunol. 59:411. (1981)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • William R. Green
    • 1
  1. 1.Program in Basic ImmunologyFred Hutchinson Cancer Research CenterSeattleUSA

Personalised recommendations