A Clone Specific Monoclonal Antibody which Inhibits T Cell-Mediated Cytolysis
Functionally defined cloned T lymphocytes represent a homogeneous population of cells uniquely suited for the analysis of cell surface molecules involved in specific allorecognition, T cell clones expressing either cytolytic or amplifier functions have been derived in this laboratory from unidirectional secondary C57BL/6 anti-DBA/2 mixed lymphocyte culture (MLC) and have been maintained in long term culture (1). One cytolytic clone, designated L3, has been found to express cytolytic activity for the H-2d gene product (2). The L3 cell line has been used in the present study to derive and characterize a monoclonal antibody which reacts specifically with that cell line, and which is capable of inhibiting antigen specific cytolysis in the absence of complement.
KeywordsLytic Activity Blocking Activity Cytolytic Activity Mixed Lymphocyte Culture P8l5 Cell
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