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The Differential Effects of Distinct Cytolysis-Inhibiting Monoclonal Antibodies on Growth and on Cytolytic Activity of T Cell Clones

  • Anne-Marie Schmitt-Verhulst
  • Pierre Golstein
  • Michel Buferne
  • Michel Pierres
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 146)

Abstract

The analysis of the involvement of cell-surface structures of cytotoxic T lympocytes (CTL) in lytic interactions with target cells has been approached in recent years by the use of antibodies capable of inhibiting cytolysis. Among antibodies found to inhibit cytolysis via their binding to the effector cells, were allogeneic anti- Lyt2 antibodies (2–6) and a xenogeneic rat anti-mouse antiserum (7). Unlike these antibodies which blocked cytolysis in the absence of added complement, antibodies raised in syngeneic mice, directed at T cell blast-specific structures possibly involved in antigen recognition, required additional complement treatment to eliminate the CTL (8,9). More recently, a systematic search has been made for cytolysis-inhibiting monoclonal antibodies (mAb) secreted by hybridoma cells obtained after fusion of rat cells immunized with mouse T cell populations (1,10–14), This has resulted in the production of mAb directed at two types of cell surface structures: (a) the Lyt2 molecule (1,10,11) and its associated Lyt3 molecule (10,11), and (b) a glycoprotein composed of 180 and 95K polypeptides defined independently by mAb LFA-1 (12,13), mAb described by Fitch and colleagues (14), and by mAb H35-89.9 described by Pierres and colleagues (1).

Keywords

Cytolytic Activity 51Cr Release Allelic Form Mixed Lymphocyte Culture Reciprocal Dilution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Anne-Marie Schmitt-Verhulst
    • 1
  • Pierre Golstein
    • 1
  • Michel Buferne
    • 1
  • Michel Pierres
    • 1
  1. 1.Centre d’ImmunologieINSERM-CNRS de Marseille-LuminyMarseille Cedex 9France

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