Functional Relationships of Lymphocyte Membrane Structures Probed with Cytolysis and/or Proliferation-Inhibiting H35-27.9 and H35-89.9 Monoclonal Antibodies
The mechanism(s) of T lymphocyte “functions” such as cytolysis (1–4) or proliferation involve those cell surface structures that insure specific recognition and may involve other cell surface structures as well. Detection of these may be via the use of monoclonal antibodies (mAb) selected for their ability to inhibit lymphocyte functions. Indeed, anti-Lyt-2 mAb have been extensively studied as to their inhibitory effect on mouse T cell-mediated cytolysis, with repeated suggestions that Lyt-2 itself may be related to the T cell specific receptor (5–10). We have developed a range of xenogeneic rat anti-mouse mAb selected for their ability to inhibit T cell-mediated cycolysis (11,12). Three of them will be used in the present report: H35-17.2 mAb, which is most probably an anti- Lyt-2 mAb, as an experimental counterpoint to the two other mAb; H35-27.9 mAb, which differs from an anti-Lyt-2 mAb at least by the tissue distribution of the structures it recognizes; and H35-89.9 mAb, which immunoprecipitates from lymphoid cell surfaces two polypeptides of 180K and 94K molecular weight.
KeywordsSpleen Cell Cytolytic Activity Cell Surface Structure Target Cell Ratio Md26 Cell
Unable to display preview. Download preview PDF.
- 4.Berke, G. Interaction of cytotoxic T l3miphocytes and target cells. Progress in Allergy 27:69 (1979).Google Scholar
- 8.Fan, J., Ahmed, A., and B. Bonavida. Studies on the induction and expression of T cell-mediated immunity. X. Inhibition by Lyt-2, 3 antisera of cytotoxic T lymphocyte-mediated anti- gen-specific and non-specific cytoxicity: evidence for the blocking of the binding between T lymphocytes and target cells and not the post-binding cytolytic steps. J. Immunol. 125: 2444 (1980).PubMedGoogle Scholar
- 11.Pierres, M., C. Goridis, and P. Golstein. Inhibition of murine T cell-mediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two l3miphoid cell surface polypeptides of 94,000 and 180,000 molecular weight. Eur. J. Immunol., in press (1981).Google Scholar
- 12.Golstein, P., and M. Pierres. Itonoclonal antibodies as probes to study the mechansim of T cell-mediated cytolysis. Proc. 14th Leuc. Cult. Conf., in press (1981).Google Scholar
- 13.Kaufmann, Y., Berke, G., and Z. Eshhar. Functional cytotoxic T lymphocyte hybridomas. Transplantation Proc. 13:1171 (1981).Google Scholar
- 17.Davignon, D., Martz E., Reynolds, T., Kürzinger, K., and T.A. Springer. MDnoclonal antibody to a novel lymphocyte function- associated antigen (LFA-1). Mechanism of blockade of T lymph- ocyte-mediated killing and effects on other T and B lymphocyte functions. J. Immunol. 127:590 (1981).PubMedGoogle Scholar
- 20.Nabholz, M., Conzelmann, A., Acuto, O., North, M., Haas, W., Pohlit, H., Von Boemer, H., Hengartner, H., Mach, J.-P., Engers, H., and J.P. Johnson. Established murine cytolytic T cell lines as tools for a somatic cell genetic analysis of T cell functions. Immunological Rev. 51:125 (1980).CrossRefGoogle Scholar