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Functional Relationships of Lymphocyte Membrane Structures Probed with Cytolysis and/or Proliferation-Inhibiting H35-27.9 and H35-89.9 Monoclonal Antibodies

  • Pierre Golstein
  • Michel Pierres
  • Anne-Marie Schmitt-Verhulst
  • Marie-Francoise Luciani
  • Michel Buferne
  • Zelig Eshhar
  • Yael Kaufmann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 146)

Abstract

The mechanism(s) of T lymphocyte “functions” such as cytolysis (1–4) or proliferation involve those cell surface structures that insure specific recognition and may involve other cell surface structures as well. Detection of these may be via the use of monoclonal antibodies (mAb) selected for their ability to inhibit lymphocyte functions. Indeed, anti-Lyt-2 mAb have been extensively studied as to their inhibitory effect on mouse T cell-mediated cytolysis, with repeated suggestions that Lyt-2 itself may be related to the T cell specific receptor (5–10). We have developed a range of xenogeneic rat anti-mouse mAb selected for their ability to inhibit T cell-mediated cycolysis (11,12). Three of them will be used in the present report: H35-17.2 mAb, which is most probably an anti- Lyt-2 mAb, as an experimental counterpoint to the two other mAb; H35-27.9 mAb, which differs from an anti-Lyt-2 mAb at least by the tissue distribution of the structures it recognizes; and H35-89.9 mAb, which immunoprecipitates from lymphoid cell surfaces two polypeptides of 180K and 94K molecular weight.

Keywords

Spleen Cell Cytolytic Activity Cell Surface Structure Target Cell Ratio Md26 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Pierre Golstein
    • 1
    • 2
  • Michel Pierres
    • 1
    • 2
  • Anne-Marie Schmitt-Verhulst
    • 1
    • 2
  • Marie-Francoise Luciani
    • 1
    • 2
  • Michel Buferne
    • 1
    • 2
  • Zelig Eshhar
    • 1
    • 2
  • Yael Kaufmann
    • 1
    • 2
  1. 1.Centre d’ImmunologieINSERM-CNRS de Marseille-LuminyMarseille Cedex 9France
  2. 2.Departments of Cell Biology and Chemical ImmunologyThe Weizmann Institute of SciencesRehovotIsrael

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