Role of Cell Motility in the Activity of Cytolytic T Lymphocytes
The distinction between specific recognition and target cell (TC) lysis has been accepted as a useful concept in the analysis of cytolytic T lymphocyte (CTL)-mediated cytotoxicity. Recently, this process has been resolved by different methods into three steps: binding; “lethal hit”; and TC lysis; the first two of which require direct CTL-TC contact (1,2). As detailed in recent reviews (3–7), CTL-TC conjugates can be isolated physically before the lethal hit step (2,8–10), or binding and lethal hit can be separated on the basis of different cations requirements (11,12), and different susceptibilities to inhibition by low temperature (15®C) and drugs (3). These different sensitivities to temperature, extracellular cations and pharmacological compounds could mean that qualitatively different intracellular activities and/or membrane-associated phenomena operate in the binding and lethal hit steps.
KeywordsConjugate Formation Specific Target Cell Binding Step Binding Area Cell Mediate Cytolysis
Unable to display preview. Download preview PDF.
- 3.Cerottini J.-C., and K.T. Brunner. Mechanisms of T and K cell- mediated cytolysis. In F. Loor and G.E. Roselants, B and T cells in immune recognition, p. 319, Wiley and Sons, Chichester, England (1977).Google Scholar
- 9.Sanderson, C.S. The mechanisms of T cell mediated cytotoxicity. II. fforphological studies on cell death by time-lapse microcinematography. Proc. Roy. Soc. Lond., Ser. B 192:241 (1976).Google Scholar
- 22.Matter, A., and P. Vassalli. Ultrastructure and fuctional study of cytotoxic T lymphocytes. G. Raspe, Advances in the biosciences 12, p. 57, Pergamon Press, Vieweg (1973).Google Scholar
- 23.Chang, T.W., and H.N. Eisen. Lymphomas with cytotoxic activity. nature 280:406 (1979).Google Scholar
- 26.Cerottini, J.-C., and K.T. Brunner. Reversible inhibition of lymphocyte-mediated cytotoxicity by cytochalasin B. Nature (New Biol.) 237:272 (1972).Google Scholar
- 31.Grimm, E.A., and B. Bonavida. Studies on the induction and expression of T-cell mediated immunity. VI. Heterogeneity of lytic efficiency exhibited by isolated cytotoxic T lymphocytes prepared from hghly enriched populations of effector-target conjugates. J. Immunol. 119:1041 (1977).PubMedGoogle Scholar
- 33.Ryser, J.-E., Rungger-Brandle, E., Chaponnier, C., Gabbiani, G., and P. Vassalli. The area of attachment of cytotoxic T lymphocytes to their target cells shows high motility and polarization of actin, but not myosin. J. Immunol, (in press) (1982)Google Scholar
- 39.Seeman, P. Ultrastructure of membrane lesions in immune lysis, osmotic lysis and drug-induced lysis. Fed. Proc. 33:216 (1974).Google Scholar