Summary
Type-I cells (from rabbit embryos) in primary cell culture were studied using the patch-clamp technique on the cell-attached configuration. Cells exhibited resting potentials of about -40 mV under normoxia. In mot cases (76%), they were gradually depolarized to 0 mV by hypoxia (PO2: 3.7 kPa). Since the input resistance of type-I cells was large (about 4 gigohm) at their resting potential, small conductance changes at this level were expected to cause such a depolarizing effect. Indeed, a single K+ channel could be recorded, which was active at -50 mV with a conductance of 137 pS and inward rectification. Its open-probability was reversibly decreased during hypoxia.
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© 1990 Plenum Press, New York
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Delpiano, M.A., Hescheler, J. (1990). Does an Inward-Rectifying K Channel Regulate Type-I Cell Response to Hypoxia?. In: Acker, H., Trzebski, A., O’Regan, R.G. (eds) Chemoreceptors and Chemoreceptor Reflexes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8938-5_2
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DOI: https://doi.org/10.1007/978-1-4684-8938-5_2
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