Nonradioactive DNA Hybridization: Application to Clinical Microbiology
While nucleic acid hybridization has been used to great benefit by molecular biologists, the application to clinical use has been limited. This is due to the requirement to label probe DNA with phosphorus-32 (32P). Since this isotope has a short half-life (14.1 days) and emits a high energy beta particle, 32P-labeled probes are short-lived and require special handling conditions and licensing. The recent development of nonradioactive DNA labeling procedures (1) presents the opportunity to apply the benefits of nucleic acid hybridization to routine clinical use. Diagnostic procedures based on this technology are being developed for clinical microbiology.
KeywordsGall Ampicillin Fluorescein Pyrimidine Thymidine
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- 10.Gillespie, D. and J. Bresser. mRNA Immunobilization in Nal: Quick-blots, Biotechniques, 1: 184, (1983).Google Scholar
- 11.Heffron. P., C. Rubens and S. Falkow. Transporttion of a plasroid DNA sequence which mediates ampicillin resistance: general description and epidemiologic considerations, in DNA Insertion Elements, Plasmids and Epsisomes, Bakhari, Shapiro and Adhya, eds. Cold Spring Harbor, New York (1977).Google Scholar
- 12.Inselburg, J. Isolation, mapping and examination of effects of TnA insertions in Col El plasmids, J. Bacterid. 129: 482, (1977).Google Scholar