Nonradioactive DNA Hybridization: Application to Clinical Microbiology

  • Norman Kelker
  • Majorie Pollice
  • Huey-Lang Yang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 224)

Abstract

While nucleic acid hybridization has been used to great benefit by molecular biologists, the application to clinical use has been limited. This is due to the requirement to label probe DNA with phosphorus-32 (32P). Since this isotope has a short half-life (14.1 days) and emits a high energy beta particle, 32P-labeled probes are short-lived and require special handling conditions and licensing. The recent development of nonradioactive DNA labeling procedures (1) presents the opportunity to apply the benefits of nucleic acid hybridization to routine clinical use. Diagnostic procedures based on this technology are being developed for clinical microbiology.

Keywords

Gall Ampicillin Fluorescein Pyrimidine Thymidine 

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Norman Kelker
    • 1
  • Majorie Pollice
    • 1
  • Huey-Lang Yang
    • 1
  1. 1.Enzo Biochem, Inc.New YorkUSA

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