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Purification and Characterization of Multiple Forms of Terminal Transferase from Human Leukemic Cells

  • Martin R. DeibelJr.
  • Mary Sue Coleman
  • John J. Hutton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)

Abstract

Terminal deoxynucleotidyl transferase catalyzes the polymerization of deoxynucleoside triphosphates onto the 3’-OH terminus of a single stranded oligo(≥3 residues) or polydeoxynucleotide initiator. This non-template directed DNA polymerase was first detected in calf thymus tissue. Terminal transferase carries out, in the presence of divalent cation (Mg+2), a linear condensation polymerization reaction which is dependent solely on the concentration of 3’-OH groups and deoxynucleotide monomer. As a biochemical reagent, terminal transferase has enjoyed widespread popularity in the synthesis of defined oligomers and polymers, in recombinant DNA technology and in sequence analysis of oligodeoxynucleotides (reviewed by Bollum, 1981).1 The enzyme was purified to apparent homogeneity from calf thymus tissue by Chang and Bollum2 and was found to have an average native molecular weight of 32,000 and subunit molecular weights in SDS of 24,000 (β) and 8000 (α).

Keywords

Acute Lymphoblastic Leukemia Chronic Myelogenous Leukemia Blast Crisis Terminal Transferase Human Thymus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Martin R. DeibelJr.
    • 1
    • 2
  • Mary Sue Coleman
    • 1
    • 2
  • John J. Hutton
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of KentuckyLexingtonUSA
  2. 2.Department of MedicineUniversity of TexasSan AntonioUSA

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