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Purification of Terminal Deoxynucleotidyl Transferase of 60,000 Dalton from Mammalian Thymus and Thymona by Immunoadsorbent Column and Comparison of Peptide Structures

  • Hiromu Nakamura
  • Kazushi Tanabe
  • Shonen Yoshida
  • Mutsushi Matsuyama
  • Toshiteru Morita
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)

Abstract

There are many discrepancies in the molecular weight of terminal deoxynucleotidyl transferase (TdT) reported, e.g. 32- and 79-kilodalton(k) forms1–7, and also in the enzyme structure, i.e. two-sub-unit form (the α and β subunits)1–3 or single polypeptide form4–7. However, it has not been established yet whether these differences reflect species- or tissue-specificities of the enzyme, or are due simply to the proteolytic degradation during purification process. In order to clarify this issue, we have developed a new, rapid purification method, immunoadsorbent column chromatography, which is suitable to purify TdT’s from limited amounts of tissues such as rodent thymus8. Furthermore, the enzyme proteins from various species of mammals were compared by two-dimensional peptide mapping.

Keywords

Tryptic Peptide Terminal Deoxynucleotidyl Transferase Enzyme Protein Single Polypeptide Normal Thymus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Hiromu Nakamura
    • 1
  • Kazushi Tanabe
    • 2
  • Shonen Yoshida
    • 3
  • Mutsushi Matsuyama
    • 4
  • Toshiteru Morita
    • 1
  1. 1.Department of Experimental RadiologyAichi Cancer Center Research InstituteChikusa-ku, Nagoya 464Japan
  2. 2.Department of BiochemistryAichi Cancer Center Research InstituteChikusa-ku, Nagoya 464Japan
  3. 3.Department of BiochemistryInstitute for Developmental Research Aichi Prefecture ColonyKasugai, Aichi 480-03Japan
  4. 4.Ultrastructure ResearchAichi Cancer Center Research InstituteChikusa-ku, Nagoya 464Japan

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