TdT-Positive and TdT-Negative Human Leukemic Cells: Specific Density and Morphology

  • M. Marini
  • G. P. Bagnara
  • G. Biagini
  • M. Baccarani
  • P. Rosito
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)


Peripheral blood cells from 9 E- and 6 E+ SIg- ALL patients and from 11 CML patients in TdT+ blastic crisis were subjected to discontinuous albumin gradient separation according to Dicke’s method, and TdT assayed in the six fractions recovered therefrom. The major results were: (i) in E- ALL and in CML-BC, TdT+ cells could be recovered either within a narrow range of specific densities or were spread over most of the gradient, which might suggest differences in cell maturation among the patients; (ii) in some instances, most leukemic blasts were found in TdT-negative or faintly positive fractions; (iii) in most, but not all, E- ALL and BC, the majority of TdT+ cells was found in low density fractions; (iv) all E+ ALL had high density TdT+ cells. Cell fractions of most patients were also examined at the electron microscope, and correlations between morphology and marker characterization were tentatively drawn.


Chronic Myeloid Leukemia Blast Cell Terminal Deoxynucleotidyl Transferase Chronic Myeloid Leukemia Patient Blast Crisis 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • M. Marini
    • 1
  • G. P. Bagnara
    • 1
  • G. Biagini
    • 2
  • M. Baccarani
    • 3
  • P. Rosito
    • 4
  1. 1.Istituto di Istologia ed Embriologia GeneraleBolognaItaly
  2. 2.Istituto di Microscopia Elettronica ClinicaBolognaItaly
  3. 3.Istituto di Ematologia “Lorenzo e Ariosto Seragnoli”Italy
  4. 4.Clinica Pediatrica Facoltà di MedicinaBolognaItaly

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