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Studies of Terminal Deoxynucleotidyl Transferase in Normal and Neoplastic Human Cells

  • Francesco Campagnari
  • Libero Clerici
  • Emilio Bombardieri
  • Paolo Vezzoni
  • Giovanni Di Fronzo
  • Maria Luisa Villa
  • Gian Luigi Buraggi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)

Abstract

Terminal deoxynucleotidyl transferase, TdT, is an unusual DNA polymerase that does not require a template and catalyzes the random addition of deoxynucleotidyl units to the 3’-OH ends of single DNA chains and of oligodeoxynucleotides. The enzyme was first identified as a distinct entity by Krakow et al. (1962), but it was purified from calf thymus and extensively characterized in the laboratory of F.J. Bollum (Yoneda and Bollum, 1965; Kato et al., 1967; Chang and Bollum, 1971a). For several years TdT was considered almost exclusively as a convenient tool for synthesizing DNA-like molecules (Bollum, 1966; Ratliff et al., 1967; Chang and Bollum, 1971b) and also we made large use of it in our Euratom program of preparing tailored polydeoxynucleotide substrates for the enzymes of DNA repair (Campagnari et al., 1973).

Keywords

Acute Lymphoblastic Leukemia Chronic Lymphocytic Leukemia Terminal Deoxynucleotidyl Transferase Immunofluorescence Test Monospecific Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Francesco Campagnari
    • 1
  • Libero Clerici
    • 1
  • Emilio Bombardieri
    • 2
  • Paolo Vezzoni
    • 2
  • Giovanni Di Fronzo
    • 2
  • Maria Luisa Villa
    • 2
  • Gian Luigi Buraggi
    • 2
  1. 1.Laboratory of Biochemistry, Biology Group D.G. XIIC.E.C. Joint Research CentreIspra (Va)Italy
  2. 2.Istituto Nazionale per lo Studio e la Cura dei TumoriMilanoItaly

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