Hybrids between Myeloma Cells and Thymocytes — An Attempt to Approach the Biological Function of Terminal Transferase

  • Catherine Transy
  • Pierre Rouget
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)


Terminal deoxynucleotidyl transferase (TdT ; nucleoside triphosphate: DNA deoxynucleotidyl-transferase, E C is an enzyme able to catalyze the polymerisation of deoxynucleotides onto a primer without requiring a template (1). During the years following its discovery (2), many data on its enzymatic properties were described, most of them being extensively reviewed by Bollum (3, 4). The TdT cellular distribution among normal adult tissues appears to be restricted to the thymus and bone marrow (5, 6). The presence of TdT was also described in peripheral cells derived from acute lymphoblastic leukemias and from lymphomas; in these cases, the cells were characterized as precursors of T or B lymphocytes (7–11). More recently, TdT was detected in transient populations of peripheric cells in young rats and mice (12), The biological function of TdT remains unknown although its biochemical properties and its cellular distribution have suggested a possible role in the differentiation of lymphoid cells and in the generation of the diversity of immunoglobulins (4, 13).


Myeloma Cell Terminal Deoxynucleotidyl Transferase Terminal Transferase Hybridation Frequency Surface Immunoglobulin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    F.J. Bollum, Oligodeoxynucleotide-primed reactions catalysed by calf thymus polymerase, J. Biol. Chem. 237:1945 (1962).PubMedGoogle Scholar
  2. 2.
    F.J. Bollum, Claf thymus polymerase, J. Biol. Chem. 235:2399 (1960).PubMedGoogle Scholar
  3. 3.
    F.J. Bollum,Terminal deoxynucleodityl transferase, in: “The Enzymes”, 3rd ed., P.D. Boyer, Ed., Academic Press, New-York, vol. 10:145 (1974).Google Scholar
  4. 4.
    F.J. Bollum, Terminal deoxynucleotidyl transferase. Biological studies, 2:11: “Advances in Enzymology”, A. Meister, Ed., Wiley- Interscience, New-York, vol. 47:347 (1978).Google Scholar
  5. 5.
    L.M.S. Chang, Development of Terminal deoxynucleotidyl transferase activity in embryonic calf thymus gland, Biochem. Biophys. Res. Commun., 44: 124 (1971).PubMedCrossRefGoogle Scholar
  6. 6.
    K.E. Gregoire, I. Goldschneider, R.W. Barton, and F.J. Bollum, Proc. Natl. Acad. Sci. USA, 74:3993 (1977).PubMedCrossRefGoogle Scholar
  7. 7.
    M.S. Coleman, J.J. Hutton, P. De Simone and F.J. Bollum, Terminal deoxynucleotidyl transferase in human leukemia, Proc. Natl. Acad. Sci. USA, 71:4404 (1974).PubMedCrossRefGoogle Scholar
  8. 8.
    R.P. McCaffrey, T.A. Harrison, R. Parkman and D. Baltimore, Terminal deoxynucleotidyl transferase activity in human leukemia cells and in normal human thymocytes, New-England J. Med., 292: 775 (1975).CrossRefGoogle Scholar
  9. 9.
    A.E. Silverstone, H. Cantor, G. Goldstein and D. Baltimore, Terminal deoxynucleotidyl-Transferase is found in prothymocytes, J. Exp. Med., 144:543 (1976).PubMedCrossRefGoogle Scholar
  10. 10.
    J.C. Brouet, J.L. Preud’homme, C. Penit, F. Valensi, P. Rouget and M. Seligmann, Acute l)miphoblastic leukemia with Pre-B-cell characteristics. Blood, 54:269 (1979).PubMedGoogle Scholar
  11. 11.
    L. Boumsell, A. Bernard, H. Coppin, Y. Richard, C. Pënit, P. Rouget, J. Lemerle and J. Dausset, Human T-cell differentiation antigens and correlation of their expression with various markers of T-cell maturation, J. Immunol. 123:2063 (1979).PubMedGoogle Scholar
  12. 12.
    R. Sasaki, F.J. Bollimi and I. Goldschneider,Transient populations of Terminal transferase positive (TdT+) cells in juvenile rats and mices, J. Immunol. 125:2501 (1980).PubMedGoogle Scholar
  13. 13.
    D. Baltimore, Is terminal deoxynucleotidyl transferase a somatic mutagen in lymphocytes ? Nature 248:409 (1974).PubMedCrossRefGoogle Scholar
  14. 14.
    E.A. Kabat, The structural basis of antibody complementarity, in: Adv. Prot. Chem., C.B. Anfinsen, J.T. Edsall, F.M. Richards, Eds., Academic Press, New-York, vol. 32:1 (1978).Google Scholar
  15. 15.
    O. Bernard, N. Hozumi, and S. Tonegawa, Sequences of Mouse immunoglobulin light chain genes before and after somatic changes, Cell, 15:1133 (1978).PubMedCrossRefGoogle Scholar
  16. 16.
    H. Sakano, R. Maki, Y. Kurosawa, W. Roeder and S. Tonegawa, Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature, 286:676 (1980).PubMedCrossRefGoogle Scholar
  17. 17.
    V.G. Seidman, E.E. Max and P. Leder, A K-immunoglobulin geneis formed by site-specific recombination without further somatic mutation. Nature, 280:370 (1979).PubMedCrossRefGoogle Scholar
  18. 18.
    P. Early, H. Huang, M. Davis, K. Calame and L. Hood, An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH,D and JH, Cell, 19:981 (1980).PubMedCrossRefGoogle Scholar
  19. 19.
    R.P. Perry, D.E. Kelley, C. Coleclough and J.F. Kearney, Organization and expression of immunoglobulins genes in fetal liver hybridomas, Proc. Natl. Acad. Sci. USA, 78:247 (1981).PubMedCrossRefGoogle Scholar
  20. 20.
    N.M. Cough and O. Bernard, Sequences of the joining region genes for immunoglobulin heavy chain and their role in generation of antibody diversity, Proc. Natl. Acad. Sci. USA, 78:509 (1981).CrossRefGoogle Scholar
  21. 21.
    S. Tonegawa, A.M. Maxam, R. Tizard, O. Bernard and W. Gilbert, Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain, Proc. Natl. Acad. Sci. USA, 75: 1485 (1978).PubMedCrossRefGoogle Scholar
  22. 22.
    D.S. Secher, C. Milstein and K. Adetugbo, Somatic mutants and antibody diversity, Immunological Rev. 36:51 (1977).CrossRefGoogle Scholar
  23. 23.
    C. Milstein,K. Adetugbo, N.J. Cowan, G. Köhler, D.S. Secher and C.D. Wilde, Somatic cell genetics of antibody-secreting cells: studies of clonal diversification and analysis by cell fusion. Cold Spring Harbor Symp. Quant. Biol., 16:793 (1976).Google Scholar
  24. 24.
    K.S. Landreth, K. McCoy, J. Clagett, F.J. Bollum and C. Rosse, Deficiency in cells expressing terminal transferase in autoimmune (motheaten) mice. Nature, 290:409 (1981).PubMedCrossRefGoogle Scholar
  25. 25.
    T.T. Wu, E.A. Kabat and H. Bilofsky, Some sequence similarities among cloned mouse DNA segments that code for X and k kight chains of immunoglobulins, Proc. Natl. Acad. Sci. USA, 76: 4617 (1979).PubMedCrossRefGoogle Scholar
  26. 26.
    M. Potter, Antigen-binding myeloma proteins of mice, Adv. in Immunol., 25:141 (1977).CrossRefGoogle Scholar
  27. 27.
    B. Goud, P. Legrain, J.C. Antoine, S. Avrameas and G. Buttin. Cross-linking of surface receptors and endocytosis of antigens are not sufficient to suppress antibody production of two hybridoma cell lines, J. Receptor Research (in press).Google Scholar
  28. 28.
    G. Buttin, C. Le Guern, L. Phalente, E.C.C. Lin, L. Ijjedrano and P.A. Cazenave, Production of hybrid lines secreting monoclonal anti-idiotypic antibodies by cell fusion on membrane filters, Curr. Topics in Microbiol, and Immunol., 81:27 (1978).Google Scholar
  29. 29.
    D. Juy, P. Legrain, P.A. Cazenave and G. Buttin, A new rapid rosette-forming cell micromethod for the detection of antibody synthesizing hybridomas, J. Immunol. Methods, 30:269 (1979).PubMedCrossRefGoogle Scholar
  30. 30.
    P. Rouget and C. Penit, Terminal deoxynucleotidyl transferase during the development of chicken thymus, cell differentiation, 9:329 (1980).PubMedCrossRefGoogle Scholar
  31. 31.
    G. Köhler, Immunoglobulin chain loss in hybridoma lines, Proc. Natl. Acad. Sci. USA, 77: 2197 (1980).PubMedCrossRefGoogle Scholar
  32. 32.
    G. Köhler, T. Pearson and C. Milstein, Fusion of T and B cells. Somatic Cell Genetics, 3:303 (1977)PubMedCrossRefGoogle Scholar
  33. 33.
    G.M. Iverson, R.A. Goldsby and L.A. Herzenberg, Expression of Thy 1.2 antigen on hybrids of B. Cells and a T lymphoma, Curr. Topics in Microbiol, and Immunol. 81:192 (1978).Google Scholar
  34. 34.
    M. J. Taussig, A. Holliman and L. J. Wright, Hybridization between T and B lymphoma cell lines. Immunology, 39:57 (1980)PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Catherine Transy
    • 1
  • Pierre Rouget
    • 1
  1. 1.Institut de Recherche en Biologie MoléculaireC.N.R.S. et Universite Paris 7Paris Cedex 05France

Personalised recommendations