Hybrids between Myeloma Cells and Thymocytes — An Attempt to Approach the Biological Function of Terminal Transferase
Terminal deoxynucleotidyl transferase (TdT ; nucleoside triphosphate: DNA deoxynucleotidyl-transferase, E C 126.96.36.199) is an enzyme able to catalyze the polymerisation of deoxynucleotides onto a primer without requiring a template (1). During the years following its discovery (2), many data on its enzymatic properties were described, most of them being extensively reviewed by Bollum (3, 4). The TdT cellular distribution among normal adult tissues appears to be restricted to the thymus and bone marrow (5, 6). The presence of TdT was also described in peripheral cells derived from acute lymphoblastic leukemias and from lymphomas; in these cases, the cells were characterized as precursors of T or B lymphocytes (7–11). More recently, TdT was detected in transient populations of peripheric cells in young rats and mice (12), The biological function of TdT remains unknown although its biochemical properties and its cellular distribution have suggested a possible role in the differentiation of lymphoid cells and in the generation of the diversity of immunoglobulins (4, 13).
KeywordsMyeloma Cell Terminal Deoxynucleotidyl Transferase Terminal Transferase Hybridation Frequency Surface Immunoglobulin
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