20α-Hydroxysteroid Dehydrogenase (20αSDH) — A New Enzymatic Marker for Pre T and T Lymphocytes
The enzyme 20αSDH which reduces progesterone to 20α-hydroxyprogesterone is found in various lymphatic organs (1,2), The following evidence suggests that within the mouse lymphocyte population, 20αSDH is confined to T lymphocytes (1,2): 20αSDH activity is found in thymocytes, splenocytes and lymph node lymphocytes from all the normal mouse strains, but not in splenocytes from congenitally athymic or neonatally thymectomized mice; it is associated with spleen cells bearing the ѳ antigen; T-cell mitogens such as Con A and PHA, alloantigens (3), but not the B cell mitogen LPS, are able to induce in vitro an increase in 20αSDH activity (2). 20αSDH is found in thymocytes and T cells from all mouse strains. In the thymus, the 20αSDH activity is located within the hydrocortisone resistant, PNA negative, medullary cells (2,4,5).
KeywordsMarrow Cell Testosterone Replacement Therapy Medullary Cell Lymphatic Organ Cortical Thymocyte
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