Murine Thymocyte Subpopulations: Two and Three Parameter Analysis with Various Markers
The thymus has two main histologically defined subdivisions, the cortex and the medulla. Many markers have been used which apparently distinguish two major lymphocyte subpopulations (in suspension) and these appear to correlate with the histological subdivision; i.e. medullary cells have one set of markers, cortical cells another set. In our initial studies of thymocyte subpopulations with some of the more commonly used markers (1), it became clear that not all reagents distinguished the same sub-populations. As a result we began an extensive review of thymocyte subpopulations using simultaneous analysis of 3 parameters (on the fluorescence activated cell sorter) in order to assess the amount of agreement between markers. We have used various combinations of monoclonal antibodies to Thy 1, Lyt 1, Lyt 2, TL3 and H-2K and fluorescent peanut agglutinin, paying particular attention to dividing cells, since the most strongly TdT positive cells are among this category. The data are too extensive to show here, but a brief summary and a specific example may be useful.
KeywordsCortical Cell Fluorescence Activate Cell Sorter Medullary Cell Fluorescence Activate Cell Sorter Analysis Terminal Transferase
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