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Ontogeny of Terminal Deoxynucleotidyl Transferase Containing Lymphocytes in Rats and Mice

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Terminal Transferase in Immunobiology and Leukemia

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 145))

Abstract

In the classic publication by L. Chang (1), the ontogenetic appearance of terminal deoxynucleotidyl transferase (TdT) was documented in the calf thymus by enzymatic analysis. TdT activity was also found in the thymus of pig, rat, rabbit, and chicken. Subsequently, TdT has been identified in high concentrations in the thymus and, in much lower concentrations, in the bone marrow of mice and human beings (2–4). Recently, minor populations of TdT-bearing cells have been demonstrated in bone marrow, spleen, liver, lung and blood of rats and mice by a sensitive and highly specific immunofluorescence assay (5–8). This technique has not only permitted direct quantification of TdT+ cells, but it has facilitated the study of their morphology, antigenic properties and anatomical distribution.

Supported in part by Grand AI-14743 from the National Institute of Allergy and Infectious Diseases, USPHS, and by Grant CH-111 from the American Cancer Society.

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References

  1. Chang, L. M. S. Development of terminal deoxynucleotidyl trans ferase activity in embryonic calf thymus gland. Biochem. Biophys. Res. Commun. 44: 124 (1971).

    Article  PubMed  CAS  Google Scholar 

  2. Coleman, M.S., Hutton, J.J., DeSimone, P., and Bollum, F. J. Terminal deoxynucleotidyl transferase in human leukemia. Proc. Nat. Acad. Sci. USA. 71: 4404 (1974).

    Article  PubMed  CAS  Google Scholar 

  3. Kung, P.C., Silverstone, A.E., McCaffrey, R.P. and Baltimore, D. Murine terminal deoxynucleotidyl transferase: Cellular distribution and response to cortisone. J. Exp. Med. 141: 855 (1975).

    PubMed  CAS  Google Scholar 

  4. Barr, R.D., Sarin, P.S., and Perry, S.M. Terminal transferase in human bone-marrow lymphocytes. Lancet i: 508 (1976).

    Article  Google Scholar 

  5. Goldschneider, I., Gregoire, K., Barton, R.W. and Bollum, F.J. Demonstration of terminal deoxynucleotidyl transferase in rat thymocytes by immunofluorence. Proc. Nat. Acad. Sci. USA. 74: 734 (1977).

    Article  PubMed  CAS  Google Scholar 

  6. Gregoire, K.E., Goldschneider, I., Barton, R.W. and Bollum, F.J. Intracellular distribution of terminal deoxynucleotidyl transferase (TdT) in rat bone marrow and thymus. Proc. Nat. Acad. Sci. USA. 74: 3993 (1977).

    Article  CAS  Google Scholar 

  7. Gregoié7e, K.E., Goldschneider, I., Barton, R.W. and Bollum, F.J. Ontogeny of terminal deoxynucleotidyl-transferase-positive cells in lymphohemopoietic tissues of rat and mouse. Immunol. 123: 1347 (1979).

    Google Scholar 

  8. Sasaki, R., Bollum, F.J., and Goldschneider, I Transient populations of terminal transferase-positive (TdT) cells in iuvenile rats and mice. Immunol. 125: 2501 (1980).

    CAS  Google Scholar 

  9. Bollum, F.J. and Goldschneider, I. Terminal deoxynucleotidyl transferase and lymphocyte differentiation. In Membranes, Receptors, and the Immune Response (Kohier, H. and Cohen, E.P., Eds.): Alan R. Liss, Inc., New York. P. 189 (1980).

    Google Scholar 

  10. Goldschneider, I. and Barton, R.W. Development and differentiation of lymphocytes. J. The Cell Surface In Animal Embryogenesis and Development (G. Poste and G.L. Nlcolson, Eds.): Elsevier, Amsterdam. P. 599 (1976).

    Google Scholar 

  11. Cantor, H. and Welssman, I. Development and function of subpopu- latlons of thymocytes and T lymphocytes. Prog. Allergy 20: 1 (1976).

    Article  PubMed  CAS  Google Scholar 

  12. Goldschneider, I., Mandel, T., and Bollum, F.J. Cyclical and reciprocal generation of terminal deoxynucleotldyl transferase positive and negative cells In explanted fetal mouse thymuses. (In preparation).

    Google Scholar 

  13. Barton, R., Goldschneider, I. and Bollum, F.J. The distribution of terminal deoxynucleotldyl transferase (TdT) among subsets of thymocytes in the rat. Immunol. 116: 462 (1976).

    CAS  Google Scholar 

  14. Rothenberg, E. Expression of differentiation antigens in subpopulations of mouse thymocytes: Regulation at the level of de novo synthesis. Cell 20: 1 (1980).

    Article  PubMed  CAS  Google Scholar 

  15. Goldschneider, I., Shortman, K., McPhee, D., Linthicum, S., Mitchell, G., Battye, F., and Bollum, F.J. Identification of subsets of proliferating low Thy 1 cells in thymus cortex and medulla (submitted for publication).

    Google Scholar 

  16. Muller, W.E., Zahn, R.K., Geurtsen, W., and Munsch, N. Agedependent alterations of DNA synthesis. Terminal deoxynucleotldyl transferase and DNA polymerase activities in bone marrow subpopulations from mice. Mech. Aging Develop. 13: 119 (1980).

    Article  CAS  Google Scholar 

  17. Pahwa, R.N., Modak, M.J., McMorrow, T., Pahwa, S., Fernandes, G., and Good, R.A. Terminal deoxynucleotldyl transferase (TdT) enzyme in the thymus and bone marrow. I. Age-associated decline of TdT in humans and mice. Cell. Immunol. 58: 39 (1981).

    Article  PubMed  CAS  Google Scholar 

  18. Goldschneider, I., Metcalf, D., Battye, F., Mandel, T. and Bollum, F.J. Analysis of rat hemopoietic cells on the fluorescence- activated cell sorter. II. Isolation of terminal deoxynucleotldyl transferase-positive cells. Exp. Med. 152: 438 (1980).

    Article  CAS  Google Scholar 

  19. Goldschneider, I., Ahmed, A., Bollum, F.J., Goldstein, A.L. Induction of terminal deoxynucleotldyl transferase and Lyt antigens with thymosin: Identification of multiple subsets of prothymocytes in mouse bone marrow and spleen. Proc. Nat. Acad. Sci. USA 78: 2469 (1980).

    Article  Google Scholar 

  20. Pazmino, N.H., Ihle, J.N., and Goldstein, A.L. Induction in vivo and vitro of terminal deoxynucleotldyl transferase by thymosin in bone marrow cells from athymic mice. Exp. Med. 147: 708 (1978).

    Article  CAS  Google Scholar 

  21. Pazmino, N.H., Ihle, J.N., McEwan, R.N., and Goldstein, A.L. Control of differentiation of thymocyte precursors in the bone marrow by thymic hormones. Cance r Treatment Rep. 62: 1749 (1978).

    CAS  Google Scholar 

  22. Hutton, J.J. and Bollum, F.J. Terminal deoxynucleotldyl trans ferase is present in athymic nude mice. Nucleic Acids Res. 4: 457 (1977).

    Article  PubMed  CAS  Google Scholar 

  23. Goldschneider, I. and Bollum, F.J, TdT-positive cells in blood of neonatal rats and mice are derived from cortical thymocytes. 4th Int. Congr. Immunol. Abst. No. 3.1.06 (1980).

    Google Scholar 

  24. Goldschneider, I. and Bollum, F.J. Antigenic profiles of termi nal deoxynucleotidyl transferase positive (TdT) cells in blood and lymphohemopoietic tissues of juvenile rats and mice, (submitted for publication).

    Google Scholar 

  25. Mason, D.W., Brideau, R.J., McMaster, W.R., Webb, M., White, R.A. and Williams, A.F. Monoclonal antibodies that define T- lymphocyte subsets in the rat. Monoclonal Antibodies (Kennett, R. H., McKearn, T. J. and Bechtol, K. B., Eds), Plenum Press, New York, P. 251 (1980).

    Google Scholar 

  26. Hayashi, J., Goldschneider, I. and Bollum, F.J. In vitro culture of terminal deoxynucleotidyl transferase-positive rat bone marrow cells. Fed. Proc. 39: 1133 (1980).

    Google Scholar 

  27. Goldschneider, I. Effects of biological response modifiers on the growth and differentiation of terminal deoxynucleotidyl transferase containing lymphocytes. (in press, these Proceedings).

    Google Scholar 

  28. Silverstone, A., Rosenberg, N., Baltimore, et al. Correlating terminal deoxynucleotidyl transferase and cell-surface markers in the pathway of lymphocyte ontogeny, Differentiation of normal and neoplastic hematopoietic cells (B. Clarkson et al., eds.). Cold Spring Harbor Laboratory, N.Y. P. 433 (1978).

    Google Scholar 

  29. Williams, A.F., Barclay, A.N., Letarte-Muirhead, M. and Morris, R.J. Rat Thy-1 antigens from thymus and brain: Their tissue distribution, purification and chemical composition. Cold Spring Harbor j BR Quant. Biol. XLI: 51 (1976).

    Google Scholar 

  30. Ritter, M.A., Gordon, L.K. and Goldschneider, I. Distribution and identity of Thy-1 bearing cells during ontogeny in rat hemopoietic and lymphoid tissues. Immunol 121: 2463 (1978).

    CAS  Google Scholar 

  31. Goldschneider, I., Metcalf, D., Battye, F. and Mandel, T. Anal ysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells. J. Exp.Med. 152: 419 (1980).

    Article  PubMed  CAS  Google Scholar 

  32. Landreth, K. S., Rosse, C., and Clagett, J. Evidence for a lineage relationship between cytoplasmic µ+ /surface µ- and surface y B lymphocytes in adult murine bone marrow. Fed. Proc. 39: 806 (1980).

    Google Scholar 

  33. Janossy, G., Bollum, F.J., Bradstock, K.F., McMichael, A., Rapson, N., and Greaves, M. F. Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia. J. Immunol 123: 1525 (1979).

    PubMed  CAS  Google Scholar 

  34. Abramson, S., Miller, R.G., and Phillips, R.A. The identifi cation in adult bone marrow of pluripotent and restricted stem cells of the myeloid and lymphoid systems. J. Exp. Med. 145: 1567 (1977).

    Article  PubMed  CAS  Google Scholar 

  35. Woan, M., McGregor, D.D., and Goldschneider, I. T-cell-mediated cytotoxicity induced by Listeria monocytogenes. III. Phenotypic characteristics of mediator T cells. (Submitted for publication),

    Google Scholar 

  36. Parkman, R., Gelfand, E.W., Rosen, F.S., Sanderson, A., and Hirshhorn, R. Severe combined immunodeficiency and adenosine deaminase. N. E. J. 292: 714 (1975).

    Article  CAS  Google Scholar 

  37. Stoop, J.W., Zegers, B.J.M., Hendricks, G.F.M., et al. Purine nucleoside Phosphorylase deficiency associated with selective cellular immunodeficiency. N. Eng. Med. 296: 651 (1977).

    Article  CAS  Google Scholar 

  38. Barton, R.W., Martiniuk, F., Hirschhorn, R., and Goldschneider, I. The distribution of adenosine deaminase among lymphocyte populations in the rat. Immunol. 122: 216 (1979).

    CAS  Google Scholar 

  39. Barton, R.W. and Goldschneider, I. Nucleotide-metabolizing enzymes and lymphocyte differentiation. Molec. Cell Biochem. 28: 135 (1979).

    Article  PubMed  CAS  Google Scholar 

  40. Barton, R.W., Martinuik, F., Hirschhorn, R., and Goldschneider, I. Inverse relationship between adenosine deaminase and purine nucleoside Phosphorylase in rat lymphocyte populations. Cell. Immunol 49: 208 (1980).

    Article  PubMed  CAS  Google Scholar 

  41. Greiner, D., Barton, R.W. and Goldschneider, I. Phenotypic characteristics of rat leukemic cell lines. Cancer Res, (in press).

    Google Scholar 

  42. Hoffbrand, A.V. Function of terminal deoxynucleotidyl transferase. (in press, these Proceedings).

    Google Scholar 

  43. Edwards, N.L., Magilavy, D.B., Cassidy, J.T., and Fox, I.H. Lymphocyte Ecto-5’-nucleotidase deficiency in agammaglobulinemia. Science 201: 628 (1978).

    Article  PubMed  CAS  Google Scholar 

  44. Barton, R.W. and Goldschneider, I. 5’-Nucleotidase activity in subpopulations of rat lymphocytes. Immunol 121: 2329 (1978).

    CAS  Google Scholar 

  45. Wortman, R.L., Mitchell, B.S., Edwards, N.L., and Fox, I.H. Biochemical basis for differentiatial deoxyadenosine toxicity to T and B lymphoblasts: Role for 5’-nucleotidase. Proc. Nat. Acad. Sei. USA 76: 2434 (1979).

    Article  Google Scholar 

  46. Dosch, H.M., Mansour, A., Cohon, A., Shore, A. and Gelfand, E.W. Inhibition of suppressor T-cell development following deoxyguanosine administration. Nature 285: 494 (1980).

    Article  PubMed  CAS  Google Scholar 

  47. Barton, R.W. and Goldschneider, I. Evidence for the cellular origin of Gross virus-induced leukemia in the rat: Description of a unique LDH isozyme sub-band in leukemia lymphoid cells and lymphohemopoietic precursor cells. Immunol. 125: 2299 (1980).

    CAS  Google Scholar 

  48. Schräder, J., Goldschneider, I., Bollum, F.J., and Schräder, S. In vitro studies of lymphocyte differentiation. II. Generation of terminal deoxynucleotidyl transferase positive cells in long term culture of mouse bone marrow. Immunol122: 2337 (1979).

    Google Scholar 

  49. Whittum, J.A., Goldschneider, I., Zurier, R.B. Effects of prostaglandin E on terminal deoxynucleotidyl transferasepositive T cell progenitors in NZB/W F1 and Balb/c mice. Fed. Proc. 39: 1130 (1980).

    Google Scholar 

  50. Greiner, D.L., Goldschneider, I., Barton, R.W. and Lubaroff, D.M. A quantitative assay system for thymocyte regeneration in the rat. Transplant. Proc. 13: 1457 (1981).

    PubMed  CAS  Google Scholar 

  51. Fathman, G.G., Small, M., Herzenberg, L.A., and Weissman, I.L. Thymus cell maturation. II. Differentiation of three “mature” subclasses in vivo. Cell Immunol. 15: 109 (1975).

    Article  PubMed  CAS  Google Scholar 

  52. Shortman, K. The pathway of T-cell development within the thymus. Prog. Immunol. 3: 197 (1977).

    Google Scholar 

  53. Burnet, F.M. The Clonal Selection Theory of Acquired Immunity. Cambridge Univ. Press, London (1959).

    Google Scholar 

  54. Jerne, N.K. The somatic generation of immune recognition. Eur. J. Immunol. 1: 1 (1971).

    Article  PubMed  CAS  Google Scholar 

  55. Chanana, A.D., Cronkite E.P., Joel, D.D., Williams, R.M., and Waksman, B. H. Migration of thymic lymphocytes: immunofluorescence and HTdR labeling studies. Adv. Exp. Med. Biol. 12: 113 (1971).

    Article  Google Scholar 

  56. Goldschneider, I. Antigenic relationship between bone marrow lymphocytes, cortical thymocytes and a subpopulation of peripheral T cells in the rat. Description of a bone marrow lymphocyte antigen. Cell Immunol. 24: 289 (1976).

    Article  PubMed  CAS  Google Scholar 

  57. Weissman, I.L., Papaioannov, V.E., and Gardner, R.L. Normal and neoplastic maturation of T lineage lymphocytes. Cold Spring Harbor Symp. Quant. Biol. 41: 9 (1977).

    Article  PubMed  Google Scholar 

  58. Durkin, H.G., Carboni, J.M. and Waksman, B.H. Antigen-induced increase in migration of large cortical thymocyte (regulatory cells) to the marginal zone and red pulp of the spleen. Immunol. 121: 1075 (1978).

    CAS  Google Scholar 

  59. Potworowski, E.F. and Nairn, R.C. Origin and fate of a thymocyte- specific antigen. Immunol. 13: 597.

    Google Scholar 

  60. Mosier, D.E. and Johnson, B.M. Ontogeny of mouse lymphocyte function. II. Development of the ability to produce antibody is modulated by T lymphocytes. J. Exp.Med. 141: 216 (1974).

    Article  Google Scholar 

  61. Droege, W. and Zucker, R. Lymphocyte subpopulations in the thymus. Transplant. Rev. 25: 3 (1975).

    PubMed  CAS  Google Scholar 

  62. Colley, D.G., Malakian, A. and Waksman, B.H. Cellular differen tiation in the thymus. II. Thymus-specific antigens in rat thymus and peripheral lymphoid cells. Immunol 104: 585 (1970).

    Google Scholar 

  63. Weissman, I.L. Thymus cell migration. Exp. Med. 126: 291 (1967).

    Article  CAS  Google Scholar 

  64. Piguiet, P.F., Irle, C., and Vassalli, P. “Early post-thymic” T cells: Peripheral T cells with the characteristics of immature thymocytes. 4th Int. Cong. Immunol. Abstr. 3: 3.1.23 (1980).

    Google Scholar 

  65. Mosier, D.E., Mathieson, B.S., Campbell, P.S. Ly phenotype and mechanism of action of mouse neonatal suppressor T cells. Exp. Med. 146: 59 (1977).

    Article  CAS  Google Scholar 

  66. Gershon, R.K., Kondo, K., and Lance, E.M. Immuno-regulatory role of spleen localizing thymocytes. J. Immunol. 112: 546 (1974).

    PubMed  CAS  Google Scholar 

  67. Polch, H. and Waksman, B.H. The splenic suppressor cell. I. Activity of thymus-dependent adherent cells: Changes with age and stress, i Immunol. 113: 127 (1974).

    Google Scholar 

  68. Small, M., Lasser-Weiss, M., and Daniel, V. Release of immature cells from the thymus during solid tumor growth: Identification by assay of TdT activity. J. Immunol. 123: 259 (1979).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, University of Connecticut Health Center, 06032, Farmington, Connecticut, USA

    Irving Goldschneider

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Editors and Affiliations

  1. CNR Institute of Biochemical and Evolutionary Genetics, Pavia, Italy

    Umberto Bertazzoni (CEC Official) (CEC Official)

  2. Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA

    F. J. Bollum

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Goldschneider, I. (1982). Ontogeny of Terminal Deoxynucleotidyl Transferase Containing Lymphocytes in Rats and Mice. In: Bertazzoni, U., Bollum, F.J. (eds) Terminal Transferase in Immunobiology and Leukemia. Advances in Experimental Medicine and Biology, vol 145. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8929-3_12

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  • DOI: https://doi.org/10.1007/978-1-4684-8929-3_12

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