Use of Immunofluorescence Tests for TdT in Human Hematologic Malignancy

  • Mary Sue Coleman
  • John J. Hutton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 145)


Terminal deoxynucleotidyl transferase (TdT) has been established as a valuable clinical tool in the differential diagnosis of acute leukemia. In pediatric disease, TdT is almost uniformly elevated in tumor cells for “T” cell and “Common” acute lymphoblastic leukemia (ALL) and in selected patients with “Pre B” ALL. In adult leukemias TdT is highly elevated in chronic myelogenous leukemia (CML in blast crisis)(30% of cases) and in undifferentiated leukemia (AUL) in acute phase (50% of cases). Patients with acute myeloid leukemia (AML) will occasionally exhibit high levels of TdT activity (10% of cases).1 Detection of TdT in leukemic cells is important clinically because it is indicative of responsiveness of the disease to chemotherapeutic protocols containing vincristine and prednisone.


Acute Myeloid Leukemia Acute Lymphoblastic Leukemia Chronic Myelogenous Leukemia Terminal Deoxynucleotidyl Transferase Blast Crisis 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Mary Sue Coleman
    • 1
    • 2
  • John J. Hutton
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of KentuckyLexingtonUSA
  2. 2.Department of MedicineUniversity of TexasSan AntonioUSA

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