Kinetics and Non-Equilibrium Ion-Exchange Dynamics
We demonstrated in the last chapter that the criteria from the equilibrium theory of sorption dynamics can only be satisfied experimentally for the chromatographic preparation of physiologically active substances (and then only approximately) given very slow solution flow rates (25–200 ml/cm2 · h). However, in these regimes and using beads 0.2–2 nm in diameter the zone boundaries are still significantly spread, even for steady-state zone and boundary migrations. The width of the zone and the extent of the boundary along the column are quite significant. A consequence of this is that the zones of closely related substances overlap.
KeywordsInternal Diffusion Elution Curve Solution Flow Rate Spherical Bead Crosslink Ratio
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