Abstract
The neuroendocrine status of Long-Evans female rats was evaluated at several key stages of reproductive senescence. Young (4–8 mo), middle-aged (10–14 mo) and old (24–30 mo) animals were studied according to reproductive state. The reproductive states studied were (1) regularly cycling, (2) constant estrus and (3) pseudopregnant, as determined by vaginal smear cytology. Neuroendocrine parameters at the levels of the hypothalamus, pituitary and steroid-producing organs were compared between each group. DA3, E and NE concentrations in the median eminence of the hypothalamus were determined by a highly sensitive radioenzymatic assay. LRF content in the median eminence was measured by radioimmunoassay. Circulating levels of LH, FSH, PRL and six steroids were determined. Changes in hormone and neurotransmitter concentrations were demonstrated in association with the various stages of reproductive senescence and with age advancement. These changes involved the hypothalamic, pituitary and steroid systems. NE content in the median eminence, FSH in serum and circulating androstenedione were all significantly increased in middle-aged, cyclic rats prior to the onset of senescent anovulation. DA concentration in 24 mo. old constant estrous rats (30.7+7.7 pg/yg, N=6) and in 30 mo. old pseudo-pregnant rats (27.5+7.1 pg/]ig, N=6) was significantly reduced compared to young (6 mo. old), cyclic controls on proestrous (55.0+4.7 pg/yg, N=12). This DA reduction was associated with a 3-fold increase in circulating prolactin. The results are discussed in terms of a regulatory cascade model of female reproductive senescence (Finch, 1976).
Rockefeller Foundation Research Fellow in Reproductive Endocrinology.
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Abbreviations
- NE:
-
norepinephrine
- E:
-
epinephrine
- DA:
-
dopamine
- LRF:
-
gonadotropin releasing factor
- PRL:
-
prolactin
- PIE:
-
prolactin inhibiting factor
- E2 :
-
estradiol
- E1 :
-
estrone
- P:
-
progesterone
- 20α-OH-P:
-
hydroxyprogesterone
- T:
-
testosterone
- A:
-
androstenedione
- HCG:
-
human choronic gonadotropin
References
Anderson, D.C., Hopper, B.R., Lasley, B.L. and Yen, S.S.C. (1976). A simple method for the assay of eight steroids in small volumes of plasma. Steroids 28, 179–196.
Anton-Tay, F., Anton, S.M. and Wurtman, R.J. (1970). Mechanism of changes in brain norepinephrine metabolism after ovariectomy. Neuro endocrinology6, 265–273.
Anton-Tay, F. and Wurtman, R.J. (1971). Brain monoamines and endocrine function. In: Frontiers in Neuro endocrinology pp. 45, eds. L. Martini and W.F. Ganong. Oxford U. Press, New York.
Aschheim, P. (1961). La pseudogestation á répétition chez les rattes séniles. C. r. Séanc. Soc. Biol.253, 1988–1990.
Aschheim, M.P. (1965). La reactivation de l’ovaire des rates séniles en oestrus permanent au moyen d’hormones gonadotropes ou de la mise a 1’obscurité. C. r. Séanc. Soc. Biol.260, 5627–5630.
Barraclough, C.A. and Sawyer, C.H. (1957). Blockade of the release of pituitary ovulating hormone in the rat by chloropromazine and reserpine: possible mechanisms of action. Endocrinology61, 341–351.
Ben-Jonathan, N. , Oliver, C, Weiner, H.J., Mical, R.S. and Porter, J.C. (1977). Dopamine in hypophysial portal plasma of the rat during the estrous cycle and throughout pregnancy. Endocrinology100, 452–458.
Ben-Jonathan, N. and Porter, J.C. (1976). A sensitive radioenzyma-tic assay for dopamine, norepinephrine and epinephrine in plasma and tissue. Endocrinology98, 1497–1507.
Birge, C.A., Jacobs, L.S., Hammer, C.T. and Daughaday, W.H. (1970). Catecholamine inhibition of prolactin secretion by isolated rat adenohypophyses. Endocrinology86, 120–130.
Blake, C.A. (1976). Effects of intravenous infusion of catecholamines on rat plasma luteinizing hormone and prolactin concentrations. Endocrinology98, 99–104.
Block, E. (1952). Quantitative morphological investigations of the follicular system in women. Acta anat.14, 108–123.
Chan, S.W.C and Leathern, J.H. (1977). Aging and ovarian steroidogenesis in the rat. J. Geront.32, 395–401.
Chiocchio, S.R., Negro-Vilar, A. and Tramezzani, J.H. (1976). Acute changes in norepinephrine content in the median eminence induced by orchidectomy or testosterone replacement. Endocrinology99, 629–635.
Clemens, J.A., Amenomori, Y., Jenkins, T. and Meites, J. (1969). Effects of hypothalamic stimulation, hormones, and drugs on ovarian function in old female rats. Proc. Soc. exp. Biol. Med.132, 561–563.
Clemens, J.A. and Bennett, D.R. (1977). Do aging changes in the preoptic area contribute to loss of cyclic endocrine function? J. Geront.32, 19–24.
Clemens, J.A. and Meites, J. (1971). Neuroendocrine status of old constant-estrous rats. Neuroendocrinology7, 249–256.
Coble, Y.D., Kohler, P.O., Gargille, C.M. and Ross, G.T. (1969). Production rates and metabolic clearance rates of human follicle-stimulating hormone in premenopausal and post-menopau-sal women. J. clin. Invest. 48, 359–363.
Crumeyrolle-Arias, M., Scheib, D. and Aschheim, O. (1976). Light and electron microscopy of the ovarian interstitial tissue in the senile rat: normal aspect and response to HCG of “Deficiency Cells” and “Epithelial Cords”. Gerontology22, 185–204.
Cuello, A.C., Hiley, R. and Iverson, L.L. (1973). Use of catechol-O-methyltransferase for the enzyme radiochemical assay of dopamine. J. Neurochem.21, 1337–1340.
Daane, T.A. and Parlow, A.F. (1971). Periovulatory patterns of rat serum follicle stimulating hormone and luteinizing hormone during the normal estrous cycle: Effects of pentobarbital. Endocrinology88, 653–663.
Dickerman, S., Kledzik, G., Gelato, M., Chen, H.J. and Meites, J. (1974). Effects of haloperidol on serum and pituitary prolactin, LH, FSH, and hypothalamic PIF and LRF. Neuroendo-crinology15, 10–20.
Ferin, M., Dyrenfurth, I., Cowchock, S., Warren, M. and Van de Weile, R.L. (1974a). Active immunization to 17β-Estradiol and its effects upon the reproductive cycle of the Rhesus monkey. Endocrinology94, 165–116.
Ferin, M., Tempone, A., Zimmering, P.E. and Van de Wiele, R.L. (1969). Effect of antibodies to 17β-Estradiol and progesterone on the estrous cycle of the rat. Endocrinology85, 1070-
Ferin, M., Warren, M., Dyrenfurth, I., Van de Wiele, R.L. and White, W.F. (1974a). Response of rhesus monkeys to LRH [LH-releasing hormone] throughout the ovarian cycle. J. clin. Endocr. Metab.38, 321–337.
Fernstrom, J.D. and Wurtman, R.J. (1977). Brain monoamines and reproductive function. In: International Review of Physiology, Reproductive Physiology II pp. 23–55 , ed. R.O. Greep, vol 13. University Park Press, Baltimore.
Finch, C.E. (1973). Catecholamine metabolism in the brains of aging male mice. Brain Res.52, 261–276.
Finch, C.E. (1976). The regulation of physiological changes during mammalian aging. Q Rev. Biol51, 49–83,
Huang, H.H., Marshall, S. and Meites, J. (1976). Induction of estrous cycles in old non-cyclic rats by progesterone, ACTH, ether stress on L-DOPA. Neuroendocrinology20, 21–34.
Huang, H.H. and Meites, J. (1975). Reproductive capacity of aging female rats. Neuro endocrinology17, 289–295.
Ingram, D.K. (1959). The vaginal smear of senile laboratory rats. J. Endocr.19, 182–188.
Judd, H.L., Judd, G.E., Lucas, W.E. and Yen, S.S.C. (1974). Endocrine function of the post-menopausal ovary: Concentration of androgens and estrogens in ovarian and peripheral vein blood. J. clin. Endoor. Metab.39, 1020–1024.
Judd, H.L., Lucas, W.E. and Yen, S.S.C. (1976). Serum 17$-estradiol and estrone levels in postmenopausal women with and without endometrial cancer. J. clin. Endoor. Metab.43, 272–278.
Judd, H.L. and Yen, S.S.C. (1973). Serum androstenedione and testosterone levels during the menstrual cycle. J. clin. Endoor. Metab.36, 475–481.
Kalra, P.S., Kalra, S.P., Krulich, L., Fawcett, C.P. and McCann, S.M. (1972). Involvement of norepinephrine in transmission of the stimulatory influence of progesterone on gonadotropin release. Endoorinology90, 1168–1176.
Kobayashi, R.M., Lu, K.H., Moore, R.Y. and Yen, S.S.C. (1978). Regional distribution of hypothalamic luteinizing hormone-releasing hormone in proestrous rats: Effects of ovariectomy and estrogen replacement. Endoorinology102, 98–105.
König, J.F.R. and Klippel, R.A.(1963). The Rat Brain, Krieger Publishing Co., New York.
Lichtensteiger, W., Felix, D., Lienhart, R. and Hefti, F. (1976). A quantitative correlation between single unit activity and fluorescence intensity of dopamine neurones in zona compacta of substantia nigra, as demonstrated under the influence of nicotine and physostigmine. Brain Res.117, 85–103.
Linnoila, M. and Cooper, R.L. (1975). Reinstatement of vaginal cycles in aged non-cycling female rats. Gerontologist15, 30.
Lofstrom, A. (1977). Catecholamine turnover alterations in discrete areas of the median eminence of the 4- and 5-day cyclic rat. Brain Res. 120, 113–131.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265–275.
Lu, K.H., Amenomori, Y., Chen, C.L. and Meites, J. (1970). Effects of central acting drugs on serum and pituitary prolactin levels in rats. Endoorinology 87, 661–615.
MacLeod, R.M. (1969). Influence of norepinephrine and catecholamine depleting agents on the synthesis and release of prolactin and growth hormone. Endoorinology85, 916–923.
Mandl, A.M. and Shelton, M. (1959). A quantitative study of oocytes in young and old nulliparous laboratory rats. J. Endoor.18, 444–450.
Meites, J., Nicoll, C.S. and Talwalker, P.K. (1963). The central nervous system and the secretion and release of prolactin. In: Advanoes in Neuroendoorinology, pp. 238–277. Ed. A.V. Nalbanov. University of Illinois Press, Urbana, 111.
Meyerson, B.J. and Sawyer, C.H. (1967). Monoamines and ovulation in the rat. Acta pharmao. tox suppl4, 18–19.
Monroe, S.E., Parlow, A.F. and Midgley, A.R. (1968). Radioimmunoassay for rat luteinizing hormone. Endoorinology83, 1004-
Neill, J.D. and Smith, M.S. (1974). Pituitary-ovarian interrelationships in the rat. In: Current Topics in ExperimentalEndocrinologys pp.73–106.Eds. James and Martini. Academic Press, New York.
Nett, T.M., Akbar, A.M., Niswender, G.D., Hedlund, M.T. and White, W.R. (1973). A radioimmunoassay for gonadotropin-releasing hormone (Gn-RH) in serum. J. clin. Endocr. Metab.36, 880–885.
Niswender, G.D., Chen, C.L., Midgley, A.R., Meites, J. and Ellis, S. (1969). Radioimmunoassay for rat prolactin. Proc. Soc. exp. Biol.130, 793–797.
Niswender, G.D., Midgley, A.R., Monroe, S.E. and Reichert, L.E. (1968). Radioimmunoassay for rat luteinizing hormone with antiovine LH serum and ovine LH-131I. Proc. Soc. exp. Biol.128, 807–811.
Novak, E.R. (1970). Ovulation after fifty. Ob. Gyn.36, 903–910.
Palkovits, M. (1973). Isolated removal of hypothalamic or other brain nuclei of the rat. Brain Res.59, 449–450.
Palkovits, M., Brownstein, M., Saavedra, J. and Axelrod, J. (1974). Norepinephrine and dopamine content of hypothalamic nuclei of the rat. Brain Res.77, 137–149.
Passon, P.G. and Peuler, J.D. (1973). A simplified radiometric assay for plasma norepinephrine and epinephrine. Analyt. Biochem.51, 618–631.
Peng, M.T. and Huang, H.H. (1972). Aging of hypothalamic-pituitary- ovarian function in the rat. Pert. Steril.23, 535–542.
Quadri, S.K., Kledzik, G.S. and Meites, J. (1973). Reinitiation of estrous cycles in old constant-estrous rats by central acting drugs. Neuro endocrinology11, 248–255.
Rubinstein, L. and Sawyer, C.H. (1970). Role of catecholamines in stimulating the release of pituitary ovulating hormone(s) in rats. Endocrinology86, 988–995.
Sawyer, C.H., Markee, J.E. and Hollinshead, W.H. (1947). Inhibition of ovulation in the rabbit by the adrenergic-blocking agent dibenamine. Endocrinology 41, 395–402.
Selmanoff, M.K., Pramik-Holdaway, M.J. and Weiner, R.I. (1976). Concentrations of dopamine and norepinephrine in discrete hypothalamic nuclei during the rat estrous cycle. Endocrinology99, 326–330.
Schally, A.V., Arimura, A., Takahara, J., Redding, T.W. and Dupont, A. (1974). Inhibition of prolactin release in vitro and invivo by catecholamines. Fedn. Proc.33, 237.
Shaar, C.J. and Clemens, J.A. (1974). The role of catecholamines in the release of anterior pituitary prolactin in vitro.Endocrinology95, 1202–1212.
Shaar, C.J., Euker, J.S., Riegle, G.D. and Meites, J. (1975). Effects of castration and gonadal steroids on serum luteinizing hormone and prolactin in old and young rats. J. Endocr.66, 45–51.
Sherman, B.M. and Korenman, S.G. (1975). Hormonal characteristics of the human menstrual cycle throughout reproductive life. J. clin. Invest.55, 699–706.
Smith, M.S., Freeman, M.E. and Neill, J.D. (1975). The control of progesterone secretion during the estrous cycle and early pseudopregnancy in the rat: Prolactin, gonadotropin and steroid levels associated with rescue of the corpus luteum of pseudopregnancy. Endocrinology96, 219–226.
Takahara, J., Arimura, A. and Schally, A.V. (1974). Suppression of prolactin release by a purified porcine PIF preparation and catecholamines infused into a rat hypophysial portal vessel. Endocrinology95, 462–465.
Talbert, G.B. (1968). Effect of maternal age on reproductive capacity. Am. J. Obstet. Gynec.102, 451–477.
Treolar, A.E., Boynton, R.E., Behn, B.G. and Brown, B.W. (1967). Variation of the human menstrual cycle through reproductive life. Int. J. Pert.12, 77–126.
Tsai, C.C. and Yen, S.S.C. (1971a). Acute effects of intravenous infusion of 17$-estradiol on gonadotropin release in pre-and post-menopausal women. J. clin. Endocr. Metab.32, 766–771.
Tsai, C.C. and Yen, S.S.C. (1971b). The effect of ethinyl estradiol administration during early follicular phase of the cycle on the gonadotropin levels and ovarian function. J. clin. Endocr. Metab.33, 917–923.
Waldi, D. (1962). Quantitative Bestimmung von Adrenalinlösungen mit Hilfe der dünnschichtchromatographischen Vergleichsmethode. Mitt. dtsch. Vharmaz. Ges.32, 125–128.
Wirz-Justice, A. (1975). Possible circadian and seasonal rhythmi-city in an in vitro model: monoamine uptake in rat brain slices. Experientia30, 1240–1241.
Wurtman, R.J., Anton-Tay, F. and Anton, S. (1969). On the use of synthesis inhibitors to estimate brain norepinephrine synthesis in gonadectomized rats. Life Sci.8, 1015–1022.
Yen, S.S.C. (1977). Regulation of the hypothalamic-pituitary-ovarian axis in women. J. Reprod. Fert.51, 181–191.
Yen, S.S.C., Tsai, C.C., Naftolin, F., Vandenberg, G. and Ajabor, L. (1972). Pulsatile patterns of gonadotropin release in subjects with and without ovarian function. J. clin. Endodr. Metab.34, 671–675.
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Wilkes, M.M., Lu, K.H., Fulton, S.L., Yen, S.S.C. (1978). Hypothalamic-Pituitary-Ovarian Interactions During Reproductive Senescence in the Rat. In: Finch, C.E., Potter, D.E., Kenny, A.D. (eds) Parkinson’s Disease—II. Advances in Experimental Medicine and Biology, vol 113. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8893-7_8
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