Staining of PO2 Measuring Points Demonstrated for the Rat Brain Cortex
The local PO2 distribution in the brain cortex is dependent upon the capillary arrangement and local blood flow, as well as the cellular distribution and rate of oxygen consumption within the tissue. As each part of the cortex is characterized by a specific cellular and capillary pattern, many authors have predicted that the local PO2 will vary accordingly (Thews, 196o; Metzger, 1976; among others). Because of technical complications however, differences in the PO2 of the various cortical layers have not yet been investigated systematically. If PO2 microelectrodes are placed according to stereotaxic coordinates large errors are to be expected. The results already published use very different criteria, such as race, sex and age as well as the histological technique used for the preparation of the tissue. In order to overcome these difficulties it is necessary to be able to localize the PO2 microelelectrode tip by combining Polarographic and histological techniques.
KeywordsAlcian Blue Local Blood Flow Histological Technique Capillary Volume Extracellular Action Potential
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