Advertisement

Simultaneous Measurement of Glucose and O2 Consumption in Cell Suspensions with Minielectrodes

  • B. T. Storey
  • K. Olofsson
  • W. Crowe
  • L. Mela
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 92)

Abstract

Separation procedures which yield cell suspensions with homogeneous cell populations greatly facilitate the study of cell metabolism, but yield a limited amount of material. Multiple recording of metabolic parameters from minimum volumes is therefore useful. We have developed a method for the simultaneous recording of glucose and O2 concentrations with Polarographic electrodes in 0.26 ml suspensions of rabbit epididymal spermatozoa (RES). These cells are found “pre-separated” as a homogeneous population in the cauda epididymidis. They consume glucose and O2 and produce lactate (Murdoch and White, 1967) and so provide a useful system to test the method as well as a system in which to study the effect of perturbations on aerobic glycolysis.

Keywords

Simultaneous Recording Aerobic Glycolysis Electrode Chamber Homogeneous Cell Population Polyethylene Centrifuge Tube 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Clark, L.C. Jr. (1956) Trans. Am. Soc. Artificial Internal Organs 2, 41.Google Scholar
  2. Clark, L.C. Jr. and Clark, E.W. (1973) Adv. Exp. Med. Biol. 37A, 127.PubMedGoogle Scholar
  3. Murdoch, R.N. and White, I.G. (1967) J. Reprod. Fert. 14, 213.Google Scholar
  4. Storey, B.T. (1975) Biol. Reprod. 13, 1.PubMedCrossRefGoogle Scholar
  5. Storey, B.T. and Kayne, F.J. (1975) Fertil. Steril. 26, 1257.PubMedGoogle Scholar
  6. Storey, B.T. and Kayne, F.J. (1977) Biol. Reprod. 16, 549.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • B. T. Storey
    • 1
  • K. Olofsson
    • 1
  • W. Crowe
    • 1
  • L. Mela
    • 1
  1. 1.University of Pennsylvania School of MedicinePhiladelphiaUSA

Personalised recommendations