Dynamic Hypoxic Hypoxemia in Brain Tissue: Experimental and Theoretical Methodologies
An experimental system was assembled to study feline cerebral cortex cellular and extracellular pO2 response to rapid changes in carotid artery oxygen levels. The system has been described in prior articles in this series and elsewhere (Bogue and Dorson, 1973; Dorson and Bogue, 1973; Dorson and Bogue, 1976; Bogue, 1974). Changes in carotid artery oxygen level could be accomplished either by varying the ventilatory gas composition or by a carotid-jugular computer controlled exchange method. The latter technique involved cannulation of both internal carotid arteries and jugular veins. All other perfusion routes were supressed by compression. Relatively open flow was allowed in both directions with exchange between venous and arterial blood on an equal volume flow basis. This system resulted in the most rapid possible input change while maintaining close to normal physiological function. Many different types of changes were investigated, and this report will concentrate on oscillatory inputs caused by both the ventilation gas and blood exchange methods. No difference in response was noted up to the upper frequency limit of the ventilation method of 0.1 Hz while the exchange method was capable of 1.0 Hz oscillations.
KeywordsBrain Blood Flow Entire Test Period Oscillatory Waveform Interstitial Fluid Compartment Normalize Brain Blood
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