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An Experimental Model of the Krogh Tissue Cylinder: Two Dimensional Quantitation of the Oxygen Gradient

  • Bjørn Quistorff
  • Britton Chance
  • Axel Hunding
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 92)

Abstract

We have observed very steep regional redox ratio transitions In Vivo in various tissues (1, 2, 3, 4). The most obvious example beeing the transition across the borderline of an ischemic area as first described by Barlow and Chance for an artificially produced infarct in a rat heart (5). We believe that the steep redox state transition may reflect similarly steep tissue oxygen gradients (6) and in order to probe that we have devised a simple model in which the ischemic borderzone in terms of oxygen gradient can be reproduced. The model is essentially a Krogh Tissue Cylinder (7) in macro scale, approximately 1:200, where the oxygen concentration profile perpendicular to the capillary may be recorded photographically in two dimensions. The model allows for independent variation of capillary oxygen tension, tissue oxygen consumption rate and facilitation of oxygen diffusion.

Keywords

Luminescence Intensity Oxygen Consumption Rate Will Emit Oxygen Gradient High Oxygen Consumption 
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References

  1. 1.
    Quistorff, B. and Chance, B. In ‘Oxygen and Physiological Function’ (F.F. Jöbsis ed.). Professional Information Library, Dallas. In Press.Google Scholar
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    Quistorff, B. and Chance, B. Am. Physiol. Soc. Meet. Chicago 1977. Abstract 1358.Google Scholar
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    Quistorff, B., Barlow, C., and Chance, B. Biophys. Soc. Meet. New Orleans 1977. Abstract F-P0S-P5.Google Scholar
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    Barlow, C. and Chance, B. Science, 193 p. 909 (1976).PubMedCrossRefGoogle Scholar
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    Chance, B. Circulation Res. Suppl. I, 38 p. 69 (1976).Google Scholar
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    Krogh, A. J. Physiol. 52 p. 409 (1918–1919).PubMedGoogle Scholar
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    Sugano, T., Oshino, N. and Chance, B. Biochem. Biophys. Acta 347 p. 340 (1974).PubMedCrossRefGoogle Scholar
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    Oshino, N., Sugano, T., Oshino, R. and Chance, B. Biochem. Biophys. Acta 368 p. 298 (1974).PubMedCrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Bjørn Quistorff
    • 1
    • 2
  • Britton Chance
    • 1
  • Axel Hunding
    • 1
    • 3
  1. 1.Johnson Research FoundationUniversity of PennsylvaniaUSA
  2. 2.Department of Biochemistry APanum InstituteDenmark
  3. 3.Institute of Chemistry, Faculty of MedicineUniversity of CopenhagenDenmark

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