Organ Absorbance and Fluorescence Spectrophotometry and its Application to Oxygen-Dependent Parameters
The application of absorbance--in contrast to reflectance--spectrophotometry to solid tissues such as the intact isolated perfused rat liver(Brauser, 1968) has opened possibilities of the detection of O2-dependent enzyme intermediates and cytochromes in the spectral region now extending from the near-infrared to the ultraviolet. Thus, by directing light of appropriate wavelengths to a lobe of perfused liver and measuring light transmitted through the tissue, considerable information has been accumulated in recent years by application of organ absorbance spectrophotometry(see below).
KeywordsPerfuse Liver Perfuse Organ Pyridine Nucleotide Fluorescence Spectrophotometry Reduce Pyridine Nucleotide
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