Organ Absorbance and Fluorescence Spectrophotometry and its Application to Oxygen-Dependent Parameters

  • Helmut Sies
  • Helmut Schwab
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 92)


The application of absorbance--in contrast to reflectance--spectrophotometry to solid tissues such as the intact isolated perfused rat liver(Brauser, 1968) has opened possibilities of the detection of O2-dependent enzyme intermediates and cytochromes in the spectral region now extending from the near-infrared to the ultraviolet. Thus, by directing light of appropriate wavelengths to a lobe of perfused liver and measuring light transmitted through the tissue, considerable information has been accumulated in recent years by application of organ absorbance spectrophotometry(see below).


Perfuse Liver Perfuse Organ Pyridine Nucleotide Fluorescence Spectrophotometry Reduce Pyridine Nucleotide 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Helmut Sies
    • 1
  • Helmut Schwab
    • 1
  1. 1.Institut für Physiologische Chemie, Physikalische Biochemie und ZellbiologieUniversität MünchenGermany

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