Application of the Optode to Measurements of Surface Po2 And Pco2 of the Isolated Guinea-Pig Heart
A few microns thick indicator layer is separated from the medium to be measured by a gas-permeable membrane which prevents possible toxic influences and spectral distortions by interactions of the indicator molecules with molecules of the substance.
The indicator film is tightly held between a quartz window (on the excitation side) and the membrane so that no changes in the indicator concentration can occur.
The optode is located in front of the biological object so that the filter effect of the tissue is avoided.
KeywordsExcitation Spectrum Biological Object Quartz Window Fluorescence Excitation Spectrum Indicator Concentration
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