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The Intracellular Heterogeneity of Oxygen Concentrations as Measured by Ultraviolet Television Microscopy of PBA Fluorescence Quenching by Oxygen

  • I. S. Longmuir
  • J. A. Knopp
  • Tei-Pei Lee
  • D. Benson
  • A. Tang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 92)

Abstract

If oxygen moves through the cell along special channels (Longmuir, 1977) and the mean solubility coefficient for oxygen in liver cells is rather low as Zander (1976) has shown, then there should be considerable intracellular heterogeneity of oxygen concentration even in a non-respiring cell with uniform PO2 throughout. Since the Polarographie electrode measures PO2 (Longmuir and Allen, 1960), this heterogeneity cannot be detected by this method. However, the fluorescence of pyrenebutyric acid (PBA) is quenched in proportion to the concentration of oxygen in a volume of a few hundred Augstrom’s radius around each photofluor molecule (Longmuir and Knopp, 1976). Thus, this method, with a theoretical geometric resolution of 0.2 μm appears to have this capability.

Keywords

Oxygen Concentration Oxygen Carrier Electrode Measure Local Solubility Solubility Coefficient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Longmuir, I.S. (1977) Search for alternative cellular oxygen carriers. In ‘Oxygen and Physiological Function1 (Jöbsis, F.F. ed.), Professional Information Library, Dallas.Google Scholar
  2. Longmuir, I.S. and Allen, F. (1960) J. Polarographic Soc. 8, 63.Google Scholar
  3. Longmuir, I.S. and Knopp, J.A. (1976) J. Appl. Physiol. 41, 798.Google Scholar
  4. Zander, R. (1976) Proc. Second OTT.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • I. S. Longmuir
    • 1
  • J. A. Knopp
    • 1
  • Tei-Pei Lee
    • 1
  • D. Benson
    • 1
  • A. Tang
    • 1
  1. 1.Department of BiochemistryNorth Carolina State UniversityRaleighUSA

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