The Intracellular Heterogeneity of Oxygen Concentrations as Measured by Ultraviolet Television Microscopy of PBA Fluorescence Quenching by Oxygen
If oxygen moves through the cell along special channels (Longmuir, 1977) and the mean solubility coefficient for oxygen in liver cells is rather low as Zander (1976) has shown, then there should be considerable intracellular heterogeneity of oxygen concentration even in a non-respiring cell with uniform PO2 throughout. Since the Polarographie electrode measures PO2 (Longmuir and Allen, 1960), this heterogeneity cannot be detected by this method. However, the fluorescence of pyrenebutyric acid (PBA) is quenched in proportion to the concentration of oxygen in a volume of a few hundred Augstrom’s radius around each photofluor molecule (Longmuir and Knopp, 1976). Thus, this method, with a theoretical geometric resolution of 0.2 μm appears to have this capability.
KeywordsOxygen Concentration Oxygen Carrier Electrode Measure Local Solubility Solubility Coefficient
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