Myoglobin Saturation and Calculated PO2 in Single Cells of Resting Gracilis Muscles
Use of myoglobin (Mb) as an indicator of intracellular PO2 has been dormant for half a century, chiefly because of difficulty in differentiating Mb from hemoglobin (Hb) when both are illuminated. We recently devised a microspectrophotometer with which light can be collected exclusively from either Hb or Mb. Spatial resolution is 2–5 μ. Since freezing arrests chemical reaction, saturation measurements on a large cell population can be interpreted as though all the measurements had been made simultaneously. In this way the purely spatial uniformity of O2 delivery can be evaluated. Measurements can be made at several loci within one cell, at various loci in a cell cluster, or in cells selected at random from grossly different regions of the muscle. The method offers the further advantage that the contribution of local capillary recruitment to O2 delivery can be evaluated.
KeywordsGracilis Muscle Saturation Measurement Spatial Uniformity Isobestic Point Frost Formation
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