Myoglobin Saturation and Calculated PO2 in Single Cells of Resting Gracilis Muscles

  • Thomas E. J. Gayeski
  • Carl R. Honig
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 92)


Use of myoglobin (Mb) as an indicator of intracellular PO2 has been dormant for half a century, chiefly because of difficulty in differentiating Mb from hemoglobin (Hb) when both are illuminated. We recently devised a microspectrophotometer with which light can be collected exclusively from either Hb or Mb. Spatial resolution is 2–5 μ. Since freezing arrests chemical reaction, saturation measurements on a large cell population can be interpreted as though all the measurements had been made simultaneously. In this way the purely spatial uniformity of O2 delivery can be evaluated. Measurements can be made at several loci within one cell, at various loci in a cell cluster, or in cells selected at random from grossly different regions of the muscle. The method offers the further advantage that the contribution of local capillary recruitment to O2 delivery can be evaluated.


Gracilis Muscle Saturation Measurement Spatial Uniformity Isobestic Point Frost Formation 
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  1. Chance, B., Oshino, N., Sugano, T. and Mayevsky, A. (1973) Adv. Exper. Med. Biol. 37A, 277.Google Scholar
  2. Coburn, R.F. and Mayers, L.B. (1971) Am. J. Physiol. 220, 66.PubMedGoogle Scholar
  3. Coburn, R.F. (1977) Adv. Exper. Med. Biol. 78, 101.Google Scholar
  4. Gayeski, T.E.J. and Honig, C.R. Resp. Physiol., Submitted.Google Scholar
  5. Honig, C.R., Frierson, J.L. and Nelson, C.N. (1971) Am. J. Physiol. 220, 357.PubMedGoogle Scholar
  6. Jöbsis, F.F. (1977) Adv. Exper. Med. Biol. 78, 3.Google Scholar
  7. Millikan, G.A. (1937) Proc. Roy. Soc. B 123, 218.CrossRefGoogle Scholar
  8. Sparks, H.V. and Mohrman, D.E. (1977) Microvasc. Research 13, 181.CrossRefGoogle Scholar
  9. Whalen, W.J., Buerk, D. and Thuning, C.A. (1973) Am. J. Physiol. 224, 763.PubMedGoogle Scholar
  10. Whalen, W.J., Buerk, D., Thuning, C., Kanoy, Jr., B.E. and Duran, W.N. (1976) Adv. Exper. Med. Biol. 75, 639.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Thomas E. J. Gayeski
    • 1
  • Carl R. Honig
    • 1
  1. 1.Department of PhysiologyUniversity of Rochester School of Medicine & DentistryRochesterUSA

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