Gene Transfer for Enhanced Growth of Livestock

  • Vernon G. Pursel
  • Caird E. RexroadJr.
  • Douglas J. Rolt


During the past decade, a series of scientific advances have provided the opportunity for the modification of domestic animals in a way that has never before been possible—that is, the ability to insert individual genes or groups of genes into living cells and have them function in a predictable manner.


Transgenic Mouse Gene Transfer Fusion Gene Growth Hormone mRNA Excess Growth Hormone 
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  1. Bolt, D. J., V. G. Pursel, R. E. Hammer, R. J. Wall, R. D. Palmiter and R. L. Brinster. 1986. Plasma concentrations of human growth hormone and porcine growth hormine in transgenic pigs. J. Anim. Sci. 63 (Suppl. 1):220 (Abstr.).Google Scholar
  2. Bradford, G. E. and T. R. Fanula. 1984. Evidence for a major gene for rapid postweaning growth in mice. Genet. Res. (Camb.) 44:293.CrossRefGoogle Scholar
  3. Brem, G., B. Brenig, H. M. Goodman, R. C. Seiden, F. Graf, B. Kruff, K. Springman, J. Hondele, J. Meyer, E. -L. Winnaker and H. Krausslich. 1985. Production of transgenic mice, rabbits and pigs by microinjection into pronuclei. Z. Zuchthygiene 20:251.CrossRefGoogle Scholar
  4. Brinster, R. L., H. Y. Chen, M. E. Trumbauer, M. K. Yagle and R. D. Palmiter. 1985. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl. Acad. Sci. USA 82:4438.PubMedCrossRefGoogle Scholar
  5. Brinster, R. L., H. Y. Chen, M. E. Trumbauer, M. K. Yagle, A. W. Senear, R. Warren and R. D. Palmiter. 1981. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell 27:223.PubMedCrossRefGoogle Scholar
  6. Brinster, R. L. and R. D. Palmiter. 1986. Introduction of genes into the germ line of animals. Harvey Lect. 80:1.Google Scholar
  7. Brinster, R. L., K. A. Ritchie, R. E. Hammer, R. L. O’Brien, B. Arp and U. Storb. 1983. Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. Nature 306:332.PubMedCrossRefGoogle Scholar
  8. Fabricant, J. D., L. C. Nuti, B. S. Minhas, W. C. Baker, J. S. Capehart, P. Marrack, J. H. Chalmers, M. W. Bradbury and J. E. Womack. 1987. Gene Transfer in goats. Theriogenology 27:229 (Abstr.).CrossRefGoogle Scholar
  9. Gordon, J. W. and F. H. Ruddle. 1981. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science 214:1244.PubMedCrossRefGoogle Scholar
  10. Gordon, J. W., G. A. Scangos, D. J. Plotkin, J. A. Barbosa and F. H. Ruddle. 1980. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA 77:7380.PubMedCrossRefGoogle Scholar
  11. Hammer, R. E., R. L. Brinster and R. D. Palmiter. 1985a. Use of gene transfer to increase animal growth. Cold Spring Harbor Symp. Quant. Biol. 50:379.CrossRefGoogle Scholar
  12. Hammer, R. E., R. D. Palmiter and R. L. Brinster. 1984. Partial correction of murine hereditary growth disorder by germ-line incorporation of a new gene. Nature 311:65.PubMedCrossRefGoogle Scholar
  13. Hammer, R. E., V. G. Pursel, C. E. Rexroad, Jr., R. J. Wall, D. J. Bolt, K. M. Ebert, R. D. Palmiter and R. L. Brinster. 1985b. Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315:680.PubMedCrossRefGoogle Scholar
  14. Hanset, R. 1982. Major genes in animal production, examples and perspectives: cattle and pigs. Proc. 2nd World Congr. Genet. Appl. Livestock Prod. VI:439.Google Scholar
  15. Khillan, J. S., P. A. Overbeek and H. Westphal. 1985. Drosophila P element integration in mouse. Dev. Biol. 109:247.PubMedCrossRefGoogle Scholar
  16. McKnight, G., R. E. Hammer, E. A. Kuenzal and R. L. Brinster. 1983. Expression of the chicken transferrin gene in transgenic mice. Cell 34:335.PubMedCrossRefGoogle Scholar
  17. Mettus, R. V., R. M. Petters, B. H. Johnson and R. M. Shuman. 1986. In vitro development of porcine blastocysts injected with a retrovirus helper cell line. J. Anim. Sci. 63 (Suppl. 1):236(Abstr.).Google Scholar
  18. Morrello, D., G. Moore, A. M. Salmon, M. Yaniv and C. Babinet. 1986. Studies on the expression of an H-2K/human growth hormone fusion gene in giant transgenic mice. EMBO J. 5:1877.Google Scholar
  19. Nancarrow, C., J. Marshall, J. Murray, I. Hazelton and K. Ward. 1987. Production of a sheep transgenic with the ovine growth hormone gene. Theriogenology 27:263 (Abstr.)CrossRefGoogle Scholar
  20. O’Hare, K. 1985. The mechanism and control of P element transposition in Drosophila melanogaster. Trends Genet. 9:250.CrossRefGoogle Scholar
  21. Palmiter, R. D., R. L. Brinster, R. E. Hammer, M. E. Trumbauer, M. G. Rosenfeld, N. C. Birnberg and R. M. Evans. 1982a. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300:611.PubMedCrossRefGoogle Scholar
  22. Palmiter, R. D., H. Y. Chen and R. L. Brinster. 1982b. Differential regulation of metallothionein-thymidine kinase fusion genes in transgenic mice and their offspring. Cell 29:710.CrossRefGoogle Scholar
  23. Palmiter, R. D., G. Norstedt, R. E. Gelinas, R. E. Hammer and R. L. Brinster. 1983. Metallo-thionein-human growth hormone fusion genes stimulate growth of mice. Science 222:809.PubMedCrossRefGoogle Scholar
  24. Paulson, K. E., N. Deka, C. W. Schmid, R. Misra, C. W. Schindler, M. G. Rush, L. Kadyk and L. Leinwand. 1985. A transposon-like element in human DNA. Nature 316:359.PubMedCrossRefGoogle Scholar
  25. Pursel, V. G., R. E. Hammer, D. J. Bolt, R. J. Wall, R. D. Palmiter and R. L. Brinster. 1986. Transgenic swine transmit foreign gene to progeny. J. Anim. Sci. 63 (Suppl. 1):203 (Abstr.).Google Scholar
  26. Pursel, V. G., K. F. Miller, C. A. Pinkert, R. D. Palmiter and R. L. Brinster. 1987a. Development of 1-cell and 2-cell pig ova after microinjection of genes. J. Anim. Sei. 65 (Suppl. 1): 402 (Abstr.).Google Scholar
  27. Pursel, V. G., C. E. Rexroad, Jr., D. J. Bolt, K. F. Miller, R. J. Wall, R. E. Hammer, C. A. Pinkert, R. D. Palmiter and R. L. Brinster. 1987b. Progress on gene transfer in farm animals. Vet. Immunol. Immunopathol. 17:303.PubMedCrossRefGoogle Scholar
  28. Robertson, E. J., A. Bradley, M. Kuehn and M. Evans. 1986. Germ line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature 323:445.PubMedCrossRefGoogle Scholar
  29. Rubenstein, J. L. R., J. F. Nicolas and F. Jacob. 1986. Introduction of genes into preimplantationmouse embryos by use of a defective recombinant retrovirus. Proc. Natl. Acad. Sci. USA 83:366.PubMedCrossRefGoogle Scholar
  30. Sejrsen, K. 1986. Endocrine mechanisms underlying genetic variation in growth in ruminants. Proc. 3rd World Congr. Genet. Appl. Livestock Prod. XI:261.Google Scholar
  31. Seiden, R. F., M. J. Skoskiewicz, K. B. Howie, P. S. Russell and H. M. Goodman. 1987. Implantation of genetically engineered fibroblasts into mice: implications for gene therapy. Science 236:714.CrossRefGoogle Scholar
  32. Smith, C. and P. R. Bampton. 1977. Inheritance of reaction to halothane anesthesia in pigs. Genet. Res. (Camb.) 29:287.CrossRefGoogle Scholar
  33. Spradling, A. C. and G. M. Rubin. 1983. The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene. Cell. 34:47.PubMedCrossRefGoogle Scholar
  34. Swift, G. H., R. E. Hammer, R. J. MacDonald and R. L. Brinster. 1984. Tissue-specific expression of the rat pancreatic elastase 1 gene in transgenic mice. Cell 38:639.PubMedCrossRefGoogle Scholar
  35. Wall, R. J., V. G. Pursel, R. E. Hammer and R. L. Brinster. 1985. Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei. Biol. Reprod. 32:645.PubMedCrossRefGoogle Scholar
  36. Watanabe, S. and H. Temin. 1983. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol. Cell. Biol. 3:2241.PubMedGoogle Scholar
  37. Wilkie, T. M., R. L. Brinster and R. D. Palmiter. 1986. Germline and somatic mosaicism in transgenic mice. Dev. Biol. 118:9.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Vernon G. Pursel
    • 1
  • Caird E. RexroadJr.
  • Douglas J. Rolt
    • 1
  1. 1.Agricultural Research Service, Beltsville Agricultural Research Center, Reproduction LaboratoryUnited States Department of AgricultureBeltsvilleUSA

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