The Phyletic Interpretation of Macromolecular Sequence Information: Sample Cases

  • Walter M. Fitch
Part of the NATO Advanced Study Institutes Series book series (NSSB, volume 14)


Methods were provided in the preceding section for the formulation of phylogenies, the reconstruction of ancestral sequences, and the counting of evolutionary changes in the context of the phylogeny, all based upon macromolecular sequences such as DNA and protein. The results of such an analysis are not facts in the sense that every detail must be a correct representation of the way it really happened. Those results are, however, chosen so that more of those details are expected to be correct than alternative formulations.


Nucleotide Substitution Codon Position Parsimonious Tree Amino Acid Replacement Ancestral Sequence 


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  1. Beintema, J.J., Gaastra, W., Lenstra, J.A., Welling, G.W. and Fitch, W.M., 1977, The Molecular Evolution of Pancreatic Ribonuclease, submitted for publication.Google Scholar
  2. Fitch, W.M., 1967, Evidence Suggesting a Non-Random Character to Nucleotide Replacements in Naturally Occurring Mutations, J. Mol. Biol., 26, 499–507.Google Scholar
  3. Fitch, W.M., 1970, Distinguishing Homologous From Analogous Proteins, Syst. Zool., 19, 99–113.Google Scholar
  4. Fitch, W.M., 1973, Is the Fixation of Observable Mutations Distributed Randomly Among the Three Nucleotide Positions of the Codon?, J. Mol. Evol., 2, 123–136.Google Scholar
  5. Fitch, W.M., 1974, A Comparison Between Evolutionary Substitutions and Variants in Human Hemoglobins, Annals N.Y. Acad. Sci., 241, 439–448.Google Scholar
  6. Fitch, W.M., 1976a, The Molecular Evolution of Cytochrome c in Eukaryotes, J. Mol. Evol., 8, 13–40.Google Scholar
  7. Fitch, W.M., 1976b, An Evaluation of Molecular Evolutionary Clocks, in Molecular Study of Biological Evolution, F.J. Ayala, ed., Sinauer Associates, Sunderland, Mass., 160–178.Google Scholar
  8. Fitch, W.M., 1977, On the Problem of Discovering the Most Parsimonious Tree, Amer. Natur., in press.Google Scholar
  9. Fitch, W.M. and Langley, C.H., 1976a, Protein Evolution and the Molecular Clock, Fed. Proc., 35, 2092–2097.Google Scholar
  10. Fitch, W.M. and Langley, C.H., 1976b, Evolutionary Rates in Proteins, Neutral Mutations and the Molecular Clock, in Progress in Molecular Anthropology, M. Goodman and R.E. Tashian,Plenum Press, N.Y., in press.Google Scholar
  11. Fitch, W.M. and Margoliash, E., 1967, A Method for Estimating the Number of Invariant Amino Acid Coding Positions in a Gene Using Cytochrome c as a Model Case, Biochem. Gen., 1, 65–71.Google Scholar
  12. Fitch, W.M. and Markowitz, E., 1970, An Improved Method for Determining Codon Variability in a Gene and Its Application to the Rate of Fixations of Mutations in Evolution, Bioch. Gen., 4, 579–593.Google Scholar
  13. Fitch, W.M. and Yasunobu, K.T., 1975, Phylogenies from Amino Acid Sequences Aligned with Gaps, J. Mol. Evol., 5, 1–24.Google Scholar
  14. Forget, B.G., Marotta, C.A., Weisman, S.M., Verma, I.M., McCaffrey, R.P. and Baltimore, D., 1974, Nucleotide Sequences of Human Globin Messenger RNA, Ann. N.Y. Acad. Sci., 241, 290–309.Google Scholar
  15. Grunstein, M., Schedl, P. and Kedes, L., 1976, Sequence Analysis and Evolution of Sea Urchin (Lytechinus pictus and Strongylocentrotus purpuratus) Histone H4 Messenger RNAs, J. Mol. Biol., 104, 351.Google Scholar
  16. Kimura, M., 1968, Evolutionary Rate at the Molecular Level, Nature, 217, 624.PubMedCrossRefGoogle Scholar
  17. Langley, C.H. and Fitch, W.M., 1973, The Constancy of Evolution: A Statistical Analysis of the a and ß Hemoglobins, Cytochrome c and Fibrinopeptide A, in Genetic Structure of Populations, Newton E. Morton, ed., Univ. Press of Hawaii, Honolulu, 246–262.Google Scholar
  18. Langley, C.H. and Fitch, W.M., 1974, An Examination of the Constancy of the Rate of Molecular Evolution, J. Mol. Evol. 3, 161–177.Google Scholar
  19. Markowitz, E., 1970, Estimation and Testing Goodness-of-Fit for Some Models of Codon Fixation Variability, Bioch. Gen., 4, 595–601.Google Scholar
  20. Salser, W., Bowen, S., Browne, D., El Adli, F., Federoff, N., Fry, K., Heindell, H., Paddock, G., Poon, R., Wallace, B. and Witcome, P., 1975, Investigation of the Organization of Mammalian Chromosomes at the DNA Sequence Level, Fed. Proc., 35, 23–35.Google Scholar
  21. Sneath, P.H.A., Sackin, M.J. and Ambler, R.P., 1975, Detecting Evolutionary Incompatibilities from Protein Sequences, Syst. Zool., 24, 311–332.Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Walter M. Fitch
    • 1
  1. 1.Dept. of Physiological ChemistryUniv. of WisconsinMadisonUSA

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