Laboratory Analysis of Split Ejaculates

  • Georges Grignon
  • André Clavert


From the works of Mac Leod and Hotchkiss in 19421 and Lundquist in 1949,2 it is generally accepted that during ejaculation in man, the accessory sex glands always secrete in the following order: Cowper’s glands, prostate, seminal vesicles. Epididymal fluid which contains the spermatozoa is liberated after release of prostatic fluid and is usually found highly concentrated in the first fraction of split ejaculate. One can therefore obtain a high concentration of male gametes to be used in artificial insemination by collecting this first fraction and thus eliminate problems associated with laboratory manipulation of the entire sample.


Seminal Vesicle Spermatozoon Motility Artificial Insemination Male Gamete Human Semen 
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  1. 1.
    Mac Leod, J. and Hotchkiss, R.S, The distribution of spermatozoa and certain chemical constituents in the human ejaculate. J. Urol., 48:225, 1942.Google Scholar
  2. 2.
    Lundquist, F. Aspects of the biochemistry of human semen. Acta Physiol. Scand., 19, suppl. 66:7, 1949.Google Scholar
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    Harvey, C. The use of proportioned ejaculates in investigation the role of accessory secretion in human semen. Stud. Fert., 8:3, 1956.Google Scholar
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    Lindholmer, C. Survival of human spermatozoa in different fractions of split ejaculates. Fertil, Steril., 24:521, 1973.Google Scholar
  5. 5.
    Lindholmer, C. and Eliasson, R. The effects of albumine, magnesium and zinc on human sperm survival in different fraction of split ejaculates. Fertil. Steril., 25:425, 1974.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Georges Grignon
    • 1
  • André Clavert
    • 2
  1. 1.Laboratoire de Biologie MédicaleFaculté de Médecine BNancyFrance
  2. 2.Laboratoire d’EmbryologieFaculté de MédecineStrasbourgFrance

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