Semen Freezing in 0.5 and 0.25 ml Straws

  • Jean-Claude Emperaire
  • Françoise Czyglik


It is striking to note that the evolution of improvements brought about in human semen freezing have followed, with a long delay, the results of veterinary research on animal breeding, mostly in the cattle. The use of liquid nitrogen, for instance, as a cooling agent for human semen, appeared only in 1963;1 at this time, the semen was still dispensed in glass ampules, although the plastic straw had been used in cattle breeding for at least ten years.2 Up to 1973, no work involved the so-called fine straw of 0.25 ml in volume which has been used with cattle since 1965.3


Artificial Insemination Conception Rate Motile Spermatozoon Human Semen Glass Ampule 
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  1. 1.
    Sherman, J.K. Improved methods of preservation of human spermatozoa by freezing and freeze-drying.Fertil. Steril., 14: 49, 1963.PubMedGoogle Scholar
  2. 2.
    Cassou, R. Congélation du sperme du taureau conditionné en paillettes de matière plastique.CR Soc. Biol., 144: 486, 1950.Google Scholar
  3. 3.
    Emperaire, J.C., Hurpy, R. and Riviere, J. Congelation comparée des spermes humain et bovin. Application à la mise au point d’une méthode de congélation du sperme humain en paillettes fines.J. Gyn. Obst. Biol. Repr., 20:707, 1973.Google Scholar
  4. 4.
    Jondet, R. Influence de la réduction du volume de la dose de sperme congelé et de sa teneur en spermatozoîdes sur la fécondation des vaches inséminées artificiellement.Proc. VI° Cong. Int. Reprod. Anim. Insem. Artif. Paris, 2:1061, 1968.Google Scholar
  5. 5.
    Jondet* R. Advances in semen preservation. In:Artificial breeding of farm animals, The Irish genesiology Society, Dublin, p. 87, 1967.Google Scholar
  6. 6.
    Behrman, S.J. and Ackerman, D.R. Freeze preservation of human semen.Amer. J. Qbstet. Gynecol. 103: 654, 1969.Google Scholar
  7. 7.
    Empereraire, J.C. and Riviere, J. La congélation du sperme humain normal.J. Gyn. Obst. Biol. Repr. 3: 215, 1974.Google Scholar
  8. 8.
    Czyglik, F. La conservation du sperme humain par congelation et ses applications.Gyn écologie27:147, 1976.Google Scholar
  9. 9.
    David, G. and Czyglik, F. Tolerance à la congélation du sperme humain en fonction de la qualité initiale du sperme.J. Gyn.Obst. Biol. Repr., 6:5–601, 1977.Google Scholar
  10. 10.
    Jondet, R. Fertilité et nombre minimal de spermatozoïdes en insemination artificielle bovine.CR Soc. Biol.. 163: 12–2678, 1969.Google Scholar
  11. 11.
    Meryman, H.T. The freezing process. In:Recent research in freezing and drying, Parkes A.S. and Smith, A. Ed., Blackwell Scientific Publications, Oxford, G.B., p. 320, 1960.Google Scholar
  12. 12.
    Lebech, P.E. Banques de sperme humain. In:F écondité et Sté-rilit é du Mâle, C. Thibault Ed., Masson et Co., Paris, France, p. 349, 1972.Google Scholar
  13. 13.
    Matheson, G.W., Carlborg, L. and Gemzell, C. Frozen human semen for artificial insemination.Amer. J. Qbstet. Gynecol., 106:495, 1969.Google Scholar
  14. 14.
    Sawada, Y. and Ackerman, D.R. Use of frozen semen. In:Progress in infertility. Behrman, S.J. and Kistner, R.W. Ed., Little Brown and Cº, Boston, p. 731, 1968.Google Scholar
  15. 15.
    Smith, K.D. and Steinberger, E. Survival of spermatozoa in a human sperm bank. Effects of long term storage in liquid nitrogen.JAMA223:7–774, 1973.Google Scholar
  16. 16.
    Ulstein, M. Fertility, motility and penetration in cervical mucus of freeze-preserved human spermatozoa.Acta Obstet.Gynec. Scand. 52:205, 1973.PubMedCrossRefGoogle Scholar
  17. 17.
    Cassou, R. Unpublished data.Google Scholar
  18. 18.
    Ansbacher, R. Artificial insemination with frozen spermatozoa.Fertil. Steril. 29:4–375, 1978.Google Scholar
  19. 19.
    Matthews, C.D., Broom, T. J., Crawshaw, K.M., Hopkins, R.E., Kerin, J.F.F. and Svigos, J.M. The influence of insemination timing and semen characteristics on the efficiency of a donor insemination program.Fertil. Steril., 29:375. 1978.Google Scholar
  20. 20.
    Schwartz, D., Mayaux, B.A., Martin-Boyce, A., Czyglik, F. and David, G. Donor insemination: Conception rate according to cycle day in a series of 821 cycles with a single insemination. Fertil. Steril., 31:226, 1979.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Jean-Claude Emperaire
    • 1
  • Françoise Czyglik
    • 2
  1. 1.Centre de Physiopathologie de la Reproduction et de la Sexualité HumaineHôpital Xavier ArnozanPessacFrance
  2. 2.Centre d’Etude et de Conservation du Sperme (CECOS)Paris-Bicêtre, Kremlin-BicêtreFrance

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